The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1

  • Meng, Qingfeng (Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University) ;
  • Gupta, Ravi (Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University) ;
  • Kwon, Soon Jae (Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University) ;
  • Wang, Yiming (Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research) ;
  • Agrawal, Ganesh Kumar (Research Laboratory for Biotechnology and Biochemistry (RLABB)) ;
  • Rakwal, Randeep (Research Laboratory for Biotechnology and Biochemistry (RLABB)) ;
  • Park, Sang-Ryeol (Gene Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University)
  • Received : 2018.01.23
  • Accepted : 2018.05.14
  • Published : 2018.08.01
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Abstract

Rice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes (P < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice.

Keywords

References

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