The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-Glyaction and Radical Scavenging Activities of Ligustri Fructus by Extraction Method

추출 방법에 따른 여정실의 최종당화산물 생성 저해 및 라디칼 소거 활성

  • Jeong, Yun Hee (Department of Food Science and Biotechnology, Daegu University) ;
  • Kim, Seo Yoon (Department of Food Science and Biotechnology, Daegu University) ;
  • Jeong, Gyeong Han (Department of Food Science and Biotechnology, Daegu University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
  • 정윤희 (대구대학교 식품공학과) ;
  • 김서윤 (대구대학교 식품공학과) ;
  • 정경한 (대구대학교 식품공학과) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2018.06.07
  • Accepted : 2018.07.25
  • Published : 2018.07.30
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Abstract

Objectives : Ligustri Fructus has been used since ancient times as a medicinal usages in folk medicines against antitumor purpose. Many biological active constituents have been identified from this biomass such as several terpenoids and lignans. In current study, the properties of antioxidant and anti-diabetic complications using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)($ABTS^+$) radicals scavenging, and advanced glycation end products (AGEs) inhibition assays were evaluated by different extraction methods of Ligustri Fructus. Methods : In present continuing research for development of bioactive natural ingredients, antioxidant and AGEs formation inhibitory capacities of Ligustri Fructus extracts using different organic solvents were prepared and the biological potentials were investigated using in vitro bioassays. Antioxidant properties were evaluated employing radical scavenging assays using DPPH and $ABTS^+$ radicals. In addition, the anti-diabetic complications effects of Ligustri Fructus extracts were tested via AGEs formation inhibitory assay. The total phenolic contents were determined using a spectrophotometric method. Results : All the tested extracts exhibited dose-dependent radical scavenging and AGEs formation inhibitory activities. Among the tested samples, hot water extract of Ligustri Fructus was showed the most potent activity with $IC_{50}$ value of $494.8{\pm}6.7{\mu}g/m{\ell}$ against DPPH radical scavenging assay. Also, $ABTS^+$ radical scavenging activity of hot water extract was higher than those of other extracts. In addition, AGEs formation inhibitory effects of each extacts and total phenolic contents were evaluated. Conclusions : These results suggested that Ligustri Fructus can be considered as a new effective source of natural antioxidant and anti-diabetic complications resources.

Keywords

References

  1. Ahmed N. Advanced glycation endproducts-role in pathology of diabetic complications. Diavetes Res Clin Pr. 2005 ; 67(1) : 3-21. https://doi.org/10.1016/j.diabres.2004.09.004
  2. Nakamura J, Kasuya Y, Hamada Y, Nakashima E, Naruse K, Yasuda Y, Hotta N. Glucose-induced hyperproliferation of cultured rat aortic smooth muscle cells through polyol pathway hyperactivity. Diabetologia. 2001 ; 44(4) : 480-487. https://doi.org/10.1007/s001250051646
  3. Huebschmann AG, Regensteiner JG, Vlassara H, Reusch JE. Diabetes and advanced glycoxidation end products. Diabetes Care. 2006 ; 29(6) : 1420-1432. https://doi.org/10.2337/dc05-2096
  4. Vlassara H. Advanced glycation end-products and atherosclerosis. Ann Med. 1996 ; 28(5) : 419-426. https://doi.org/10.3109/07853899608999102
  5. Matsuda H, Wang T, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of protein glycation and radical scavenging activities. Bioorg Med Chem. 2003 ; 11(24) : 5317-5323. https://doi.org/10.1016/j.bmc.2003.09.045
  6. Edelstein D, Brownlee M. Mechanistic studies of advanced glycosylation end product inhibition by aminoguanidine. Diabetes. 1992 ; 41(1) : 26-29. https://doi.org/10.2337/diab.41.1.26
  7. Ceriello A. New insights on oxidative stress and diabetic complications may lead to a “causal” antioxidant therapy. Diabetes care, 2003 ; 26(5) : 1589-1596. https://doi.org/10.2337/diacare.26.5.1589
  8. Stitt A, Gardiner TA, Anderson NL, Canning P, Frizzell N, Duffy N, Thorpe SR. The AGE inhibitor pyridoxamine inhibits development of retinopathy in experimental diabetes. Diabetes. 2002 ; 51(9) : 2826-2832. https://doi.org/10.2337/diabetes.51.9.2826
  9. Jeong GH, Park EK, Kim TH. New anti-glycative flavonoids from Cirsium setidens with potent radical scavenging activities. Phytochem Lett. 2018 ; 26 : 115-119. https://doi.org/10.1016/j.phytol.2018.05.029
  10. Jang DS, Kim JM, Lee YM, Kim YS, Kim JH, Kim JS. Puerariafuran, a new inhibitor of advanced glycation end products (AGEs) isolated from the roots of Pueraria lobata. Chem Pharm Bull. 2006 ; 54(9) : 1315-1317. https://doi.org/10.1248/cpb.54.1315
  11. Lee YS, Kang YH, Jung JY, Lee S, Ohuchi K, Shin KH, Lim SS. Protein glycation inhibitors from the fruiting body of Phellinus linteus. Biol Pharm Bull. 2008 ; 31(10) : 1968-1972. https://doi.org/10.1248/bpb.31.1968
  12. Zhang Y, Lai WP, Leung PC, Wu CF, Yao XS, Wong MS. Effects of Fructus Ligustri Lucidi extract on bone turnover and calcium balance in ovariectomized rats. Biol Pharm Bull. 2006 ; 29(2) : 291-296. https://doi.org/10.1248/bpb.29.291
  13. Ngo QMT, Lee HS, Nguyen VT, Kim JA, Woo MH, Min BS. Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation. Phytochemistry. 2017 ; 141 : 147-155. https://doi.org/10.1016/j.phytochem.2017.06.001
  14. Zhang DF, Huang W, Huang JQ, Zhang RL, Liao ZQ. Study on proliferation inhibition and antiinvasion and apoptotic induction of oleanolic acid in human lung cancer cell line. Cancer Prev Res. 2003 ; 30 : 180-182.
  15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965 ; 16(3) : 144-158.
  16. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958 ; 181(4617) : 1199-1200. https://doi.org/10.1038/1811199a0
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS+ radical cation decolorization assay. Free Radic Biol Med. 1999 ; 26(9) : 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Vinson JA, Howard TB. Inhibition of protein glycation and advanced glycation end products by ascorbic acid and other vitamins and nutrients. J. Nutr. Biochem. 1996 ; 7(12) : 659-663. https://doi.org/10.1016/S0955-2863(96)00128-3
  19. Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial byproducts: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006 ; 99(1) : 191-203. https://doi.org/10.1016/j.foodchem.2005.07.042
  20. Bravo L. Polyphenols: chemistry, dietary sources, metablism, and nutritional significance. Nutr Rev. 1998 ; 56(11) : 317-333. https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
  21. Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry. 2000 ; 55(6) : 481-504. https://doi.org/10.1016/S0031-9422(00)00235-1