대한본초학회지 (The Korea Journal of Herbology)

  • 제33권4호
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  • Pages.59-67
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  • 2018
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

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TNF-α로 유도된 혈관내피세포의 혈관염증에 미치는 오적산(五積散)의 억제 효과

Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

  • 한병혁 (원광대학교 한의과대학 생리학교실) ;
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 김혜윰 (원광대학교 한의과대학 생리학교실) ;
  • 안유미 (원광대학교 한의과대학 생리학교실) ;
  • 홍미현 (원광대학교 한의과대학 생리학교실) ;
  • 손찬옥 (원광대학교 한의과대학 생리학교실) ;
  • 나세원 (원광대학교 한의과대학 생리학교실) ;
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 강대길 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실)
  • Han, Byung Hyuk (Department of Physiology, College of Korean Medicine) ;
  • Yoon, Jung Joo (Department of Physiology, College of Korean Medicine) ;
  • Kim, Hye Yoom (Department of Physiology, College of Korean Medicine) ;
  • Ahn, You Mee (Department of Physiology, College of Korean Medicine) ;
  • Hong, Mi Hyeon (Department of Physiology, College of Korean Medicine) ;
  • Son, Chan Ok (Department of Physiology, College of Korean Medicine) ;
  • Na, Se Won (Department of Physiology, College of Korean Medicine) ;
  • Lee, Yun Jung (Department of Physiology, College of Korean Medicine) ;
  • Gang, Dae-Gil (Department of Physiology, College of Korean Medicine) ;
  • Lee, Ho Sub (Department of Physiology, College of Korean Medicine)
  • 투고 : 2018.06.04
  • 심사 : 2018.07.25
  • 발행 : 2018.07.30
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초록

Objectives : Ojeoksan, originally recorded in an ancient Korean medicinal book named "Donguibogam" and has been used for the treatment of circulation disorder of blood which was called blood accumulation (血積) in Korean medicine. Therefore, this study was carried out to investigate the beneficial effect of OJS on vascular inflammation in HUVECs. Methods : We evaluated the effect of OJS on the expression of cell adhesion molecules and protective role in HUVEC stimulated by TNF-${\alpha}$ by using Western blot. Results : Pretreatment with OJS decreased the adhesion of HL-60 cells to TNF-${\alpha}$-induced HUVEC. OJS suppressed TNF-${\alpha}$-induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and endothelial cell selectin (E-selectin). Moreover, OJS significantly decreased TNF-${\alpha}$-induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of $I{\kappa}B-{\alpha}$ in the cytoplasm compared to the experimental group. Pretreatment with OJS inhibited the trans-location of NF-${\kappa}B$ p65 to the nucleus. OJS also inhibited phosphorylation of MAPKs compared to the experimental group. OJS significantly increased the protein expression of Nrf2 and HO-1. Conclusions : Ojeoksan has a protective effect on vascular inflammation, and might be a potential therapeutic agent for early atherosclerosis.

키워드

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