The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

TNF-α로 유도된 혈관내피세포의 혈관염증에 미치는 오적산(五積散)의 억제 효과

  • Han, Byung Hyuk (Department of Physiology, College of Korean Medicine) ;
  • Yoon, Jung Joo (Department of Physiology, College of Korean Medicine) ;
  • Kim, Hye Yoom (Department of Physiology, College of Korean Medicine) ;
  • Ahn, You Mee (Department of Physiology, College of Korean Medicine) ;
  • Hong, Mi Hyeon (Department of Physiology, College of Korean Medicine) ;
  • Son, Chan Ok (Department of Physiology, College of Korean Medicine) ;
  • Na, Se Won (Department of Physiology, College of Korean Medicine) ;
  • Lee, Yun Jung (Department of Physiology, College of Korean Medicine) ;
  • Gang, Dae-Gil (Department of Physiology, College of Korean Medicine) ;
  • Lee, Ho Sub (Department of Physiology, College of Korean Medicine)
  • 한병혁 (원광대학교 한의과대학 생리학교실) ;
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 김혜윰 (원광대학교 한의과대학 생리학교실) ;
  • 안유미 (원광대학교 한의과대학 생리학교실) ;
  • 홍미현 (원광대학교 한의과대학 생리학교실) ;
  • 손찬옥 (원광대학교 한의과대학 생리학교실) ;
  • 나세원 (원광대학교 한의과대학 생리학교실) ;
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 강대길 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실)
  • Received : 2018.06.04
  • Accepted : 2018.07.25
  • Published : 2018.07.30
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Abstract

Objectives : Ojeoksan, originally recorded in an ancient Korean medicinal book named "Donguibogam" and has been used for the treatment of circulation disorder of blood which was called blood accumulation (血積) in Korean medicine. Therefore, this study was carried out to investigate the beneficial effect of OJS on vascular inflammation in HUVECs. Methods : We evaluated the effect of OJS on the expression of cell adhesion molecules and protective role in HUVEC stimulated by TNF-${\alpha}$ by using Western blot. Results : Pretreatment with OJS decreased the adhesion of HL-60 cells to TNF-${\alpha}$-induced HUVEC. OJS suppressed TNF-${\alpha}$-induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and endothelial cell selectin (E-selectin). Moreover, OJS significantly decreased TNF-${\alpha}$-induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of $I{\kappa}B-{\alpha}$ in the cytoplasm compared to the experimental group. Pretreatment with OJS inhibited the trans-location of NF-${\kappa}B$ p65 to the nucleus. OJS also inhibited phosphorylation of MAPKs compared to the experimental group. OJS significantly increased the protein expression of Nrf2 and HO-1. Conclusions : Ojeoksan has a protective effect on vascular inflammation, and might be a potential therapeutic agent for early atherosclerosis.

Keywords

References

  1. Tay SS, Mc Cormack A, Rose ML. Effect of cognate human CD4+ Tcell and endothelial cell interactions upon chemokine production. Transplantation. 2004; 78(7): 987-94. https://doi.org/10.1097/01.TP.0000137205.26405.40
  2. Uzel G, Pachman LM. Cytokines in juvenile dermatomyositis pathophysiology: potential and challenge. Curr Opin Rheumatol. 2003; 15(6): 691-7. https://doi.org/10.1097/00002281-200311000-00003
  3. Liu X, Pan L, Wang X, Gong Q, Zhu YZ. Leonurine protects against tumor necrosis factor-${\alpha}$-mediated inflammation in human umbilical vein endothelial cells. Atherosclerosis. 2012; 222: 34-42. https://doi.org/10.1016/j.atherosclerosis.2011.04.027
  4. Rao RM, Yang L, Garcia-Cardena G, Liscinskas FW. Endothelial-dependent mechanisms of leukocyte recruitment to the vascular wall. Circ Res. 2007; 101: 234-47. https://doi.org/10.1161/CIRCRESAHA.107.151860b
  5. Sana TR, Janatpour MJ, Sathe M, McEvoy LM, McClanahan TK. Microarray analysis of primary endothelial cells challenged with different inflammatory and immune cytokines. Cytokine. 2005; 29: 256-69.
  6. Gloire G, Legrand-Poels S, Piette J. NF-kappaB activation by reactive oxygen species: fifteen years later. Biochem Pharmacol. 2006; 72(11): 1493-505. https://doi.org/10.1016/j.bcp.2006.04.011
  7. Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal. 2001; 13(2): 85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  8. Kumar S, Sharma A, Madan B, Singhal V, Ghosh B. Isoliquiritigenin inhibits IKappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells. Biochem Pharmacol. 2007; 73: 1602-12. https://doi.org/10.1016/j.bcp.2007.01.015
  9. Li L, Kobayashi M, Kaneko H, Nakajima-Takagi Y, Nakayama Y, Yamamoto M. Molecular evolution of Keap1. Two Keap1 molecules with distinctive intervening region structures are conserved among fish. J Biol Chem. 2008; 283: 3248-55. https://doi.org/10.1074/jbc.M708702200
  10. Liu XM, Peyton KJ, Ensenat D, Wang H, Hannink M, Alam J, Durante W. Nitric oxide stimulates heme oxygenase-1 gene transcription via the Nrf2/ARE complex to promote vascular smooth muscle cell survival. Cardiovasc Res. 2007; 75: 381-89. https://doi.org/10.1016/j.cardiores.2007.03.004
  11. Alam J, Cook JL. Transcriptional regulation of the heme oxygenase-1 gene via the stress response element pathway. Curr Pharm Des. 2003; 9(30): 2499-511. https://doi.org/10.2174/1381612033453730
  12. The Korean Journal of Oriental Medical Prescription. Herbal Formula Science. A Prescriptional Study of Ojeoksan on Clinical Application. 2012: 20(2): 153-64.
  13. Lee SI. CheonJinChubangHaesul. Seoul : Seongbosa. 1987 : 118
  14. National Health Insurance Corporation, Health Insurance Review & Assessment Service. National Health Insurance statistical yearbook. National Health Insurance Corporation and Health Insurance Review and Assessment Service. 2010: 300-1.
  15. Lee JT. Research on intake of Chinese medicine by Korean. Korea Food and Drug Administration. 2006: 94.
  16. Bak YH, Huang DS, Shin HK. The analysis of herbal medicine preparations equipped in traditional Korean medical clinics. The Korean J of Oriental Med Prescrip. 2010; 18(1): 43-56.
  17. Tuttolomondo A, Pecoraro R, Pinto A. Studies of selective TNF inhibitors in the treatment of brain injury from stroke and trauma: a review of the evidence to date. Drugs Des Devel Ther. 2014; 7(8): 2221-38.
  18. Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA. Induction by IL-1 and interferon gamma: tissue distribution, biochemistry, function of a natural adherence molecule (ICAM-1). J Immunol. 1986; 137(1): 245-54.
  19. Cleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK. Efficient large-scale production and concentration of HIV-1-based lentivial vectors for use in vivo. Physiol. 2003; 12: 221-8.
  20. Luo SF, Wang CC, Chen CS, Helenius LD, Yang CM. Induction of cycloocygenase-2 by lipopolysaccharide in canine tracheal smooth muscle cells : involvement of p 42, 44 and p 38 mitogen-activated protein kinases and nuclear factor-kappa B pathways. Cell Signal. 2003; 15: 497-509. https://doi.org/10.1016/S0898-6568(02)00135-3
  21. Korhonen P, Helenius M, Salminen A. Age-related changes in the regulation of transcription factor NF-kappaB in rat brain. Neurosci Lett. 1997; 255: 61-4.
  22. Lawrence T, Gilory DW. Possible new role for NF-${\kappa}B$ in the resolution of inflammation. Nat Med. 2001; 7: 1291-7. https://doi.org/10.1038/nm1201-1291
  23. Brasier AR. The nuclear factor-kappaBinterleukin-6 signaling pathway mediating vascular inflammation. Cardiovasc Res. 2010; 86: 211-8 https://doi.org/10.1093/cvr/cvq076
  24. A Banerjee, S Gerondakis. Coordinating TLRactivated signaling pathways in cells of the immune system Immunol. Cell Biol. 2007; 85: 420-4.
  25. Frantz S, Kobzik L, Kim YD, Fukazawa R, Medzhitov R, Lee RT. Toll4 (TLR4) expression in cardiac myocytes in normal and failing myocardium. J Clin Invest. 1999; 104(3): 271-80. https://doi.org/10.1172/JCI6709
  26. Y Liu, H Yin, M Zhao, Q. LuTLR2 and TLR4 in autoimmune diseases: a comprehensive reviewClin. Rev Allergy Immunol. 2014; 47: 136-47. https://doi.org/10.1007/s12016-013-8402-y
  27. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res. 2001; 480-481: 243-268 https://doi.org/10.1016/S0027-5107(01)00183-X
  28. Chen JW, Chen YH, Lin FY, Chen YL, Lin SJ. Ginkgo biloba extract inhibits tumor necrosis factor-alpha-induced reactive oxygen species generation, transcription factor activation, and cell adhesion molecule expression in human aortic endothelial cells. Arterioscler Thromb Vasc Biol. 2003; 23: 1559-66. https://doi.org/10.1161/01.ATV.0000089012.73180.63
  29. Kato H, Amersi F, Buelow R, Melinek J, Coito AJ, Ke B, Busuttil RW, Kupiec-Weglinski J. Heme oxygenase-1 overexpression protects rat livers from ische