The korean journal of orthodontics (대한치과교정학회지)

The Korean Association Of Orthodontists (대한치과교정학회)
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Effect of caspases and RANKL induced by heavy force in orthodontic root resorption

  • Minato, Yukari (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Yamaguchi, Masaru (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Shimizu, Mami (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Kikuta, Jun (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Hikida, Takuji (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Hikida, Momoko (Department of Orthodontics, Nihon University School of Dentistry at Matsudo) ;
  • Suemitsu, Masaaki (Department of Oral Pathology, Nihon University School of Dentistry at Matsudo) ;
  • Kuyama, Kayo (Department of Oral Pathology, Nihon University School of Dentistry at Matsudo) ;
  • Kasai, Kazutaka (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
  • Received : 2017.08.22
  • Accepted : 2017.12.08
  • Published : 2018.07.25
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Abstract

Objective: Orthodontic root resorption (ORR) due to orthodontic tooth movement is a difficult treatment-related adverse event. Caspases are important effector molecules for apoptosis. At present, little is known about the mechanisms underlying ORR and apoptosis in the cementum. The aim of the present in vivo study was to investigate the expression of tartrate-resistant acid phosphatase (TRAP), caspase 3, caspase 8, and receptor activator of nuclear factor kappa-B ligand (RANKL) in the cementum in response to a heavy or an optimum orthodontic force. Methods: The maxillary molars of male Wistar rats were subjected to an orthodontic force of 10 g or 50 g using a closed coil spring. The rats were sacrificed each experimental period on days 1, 3, 5, and 7 after orthodontic force application. And the rats were subjected to histopathological and immunohistochemical analyses. Results: On day 7 for the 50-g group, hematoxylin and eosin staining revealed numerous root resorption lacunae with odontoclasts on the root, while immunohistochemistry showed increased TRAP- and RANKL-positive cells. Caspase 3- and caspase 8-positive cells were increased on the cementum surfaces in the 50-g group on days 3 and 5. Moreover, the number of caspase 3- and caspase 8-positive cells and RANKL-positive cells was significantly higher in the 50-g group than in the 10-g group. Conclusions: In our rat model, ORR occurred after apoptosis was induced in the cementum by a heavy orthodontic force. These findings suggest that apoptosis of cementoblasts is involved in ORR.

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References

  1. Kaley J, Phillips C. Factors related to root resorption in edgewise practice. Angle Orthod 1991;61:125-32.
  2. Linge BO, Linge L. Apical root resorption in upper anterior teeth. Eur J Orthod 1983;5:173-83. https://doi.org/10.1093/ejo/5.3.173
  3. Owman-Moll P, Kurol J, Lundgren D. The effects of a four-fold increased orthodontic force magnitudeon tooth movement and root resorptions. An intraindividual study in adolescents. Eur J Orthod 1996;18:287-94. https://doi.org/10.1093/ejo/18.1.287
  4. Owman-Moll P, Kurol J, Lundgren D. Effects of a doubled orthodontic force magnitude on tooth movement and root resorptions. An inter-individual study in adolescents. Eur J Orthod 1996;18:141-50. https://doi.org/10.1093/ejo/18.1.141
  5. Chan E, Darendeliler MA. Physical properties of root cementum: Part 5. Volumetric analysis of root resorption craters after application of light and heavy orthodontic forces. Am J Orthod Dentofacial Orthop 2005;127:186-95. https://doi.org/10.1016/j.ajodo.2003.11.026
  6. Hikida T, Yamaguchi M, Shimizu M, Kikuta J, Yoshino T, Kasai K. Comparisons of orthodontic root resorption under heavy and jiggling reciprocating forces during experimental tooth movement in a rat model. Korean J Orthod 2016;46:228-41. https://doi.org/10.4041/kjod.2016.46.4.228
  7. Baud V, Karin M. Signal transduction by tumor necrosis factor and its relatives. Trends Cell Biol 2001;11:372-7. https://doi.org/10.1016/S0962-8924(01)02064-5
  8. Hamaya M, Mizoguchi I, Sakakura Y, Yajima T, Abiko Y. Cell death of osteocytes occurs in rat alveolar bone during experimental tooth movement. Calcif Tissue Int 2002;70:117-26. https://doi.org/10.1007/s002230010021
  9. Rana MW, Pothisiri V, Killiany DM, Xu XM. Detection of apoptosis during orthodontic tooth movement in rats. Am J Orthod Dentofacial Orthop 2001;119:516-21. https://doi.org/10.1067/mod.2001.113654
  10. Wu CC, Lin JP, Yang JS, Chou ST, Chen SC, Lin YT, et al. Capsaicin induced cell cycle arrest and apoptosis in human esophagus epidermoid carcinoma CE 81T/VGH cells through the elevation of intracellular reactive oxygen species and Ca2+ productions and caspase-3 activation. Mutat Res 2006;601:71-82. https://doi.org/10.1016/j.mrfmmm.2006.06.015
  11. Diercke K, Zingler S, Kohl A, Lux CJ, Erber R. Gene expression profile of compressed primary human cementoblasts before and after IL-$1{\beta}$ stimulation. Clin Oral Investig 2014;18:1925-39. https://doi.org/10.1007/s00784-013-1167-0
  12. Nakano Y, Yamaguchi M, Fujita S, Asano M, Saito K, Kasai K. Expressions of RANKL/RANK and M-CSF/c-fms in root resorption lacunae in rat molar by heavy orthodontic force. Eur J Orthod 2011;33:335- 43. https://doi.org/10.1093/ejo/cjq068
  13. Hatai T, Yokozeki M, Funato N, Baba Y, Moriyama K, Ichijo H, et al. Apoptosis of periodontal ligament cells induced by mechanical stress during tooth movement. Oral Dis 2001;7:287-90. https://doi.org/10.1034/j.1601-0825.2001.00663.x
  14. Asano M, Yamaguchi M, Nakajima R, Fujita S, Utsunomiya T, Yamamoto H, et al. IL-8 and MCP- 1 induced by excessive orthodontic force mediates odontoclastogenesis in periodontal tissues. Oral Dis 2011;17:489-98. https://doi.org/10.1111/j.1601-0825.2010.01780.x
  15. Boatright KM, Renatus M, Scott FL, Sperandio S, Shin H, Pedersen IM, et al. A unified model for apical caspase activation. Mol Cell 2003;11:529-41. https://doi.org/10.1016/S1097-2765(03)00051-0
  16. Mabuchi R, Matsuzaka K, Shimono M. Cell proliferation and cell death in periodontal ligaments during orthodontic tooth movement. J Periodontal Res 2002;37:118-24. https://doi.org/10.1034/j.1600-0765.2001.10602.x
  17. Zheng L, Wang W, Ni J, Mao X, Song D, Liu T, et al. Role of autophagy in tumor necrosis factor-${\alpha}$- induced apoptosis of osteoblast cells. J Investig Med 2017;65:1014-20. https://doi.org/10.1136/jim-2017-000426
  18. Mitsuhashi M, Yamaguchi M, Kojima T, Nakajima R, Kasai K. Effects of HSP70 on the compression force- induced TNF-${\alpha}$ and RANKL expression in human periodontal ligament cells. Inflamm Res 2011; 60:187-94. https://doi.org/10.1007/s00011-010-0253-x
  19. Matsumoto Y, Sringkarnboriboon S, Ono T. Proinflammatory mediators related to orthodonticallyinduced periapical root resorption in rat mandibular molars. Eur J Orthod 2017;39:686-91. https://doi.org/10.1093/ejo/cjx033
  20. Szymczyk KH, Freeman TA, Adams CS, Srinivas V, Steinbeck MJ. Active caspase-3 is required for osteoclast differentiation. J Cell Physiol 2006;209: 836-44. https://doi.org/10.1002/jcp.20770
  21. Kagayama M, Sasano Y, Mizoguchi I, Takahashi I. Confocal microscopy of cementocytes and their lacunae and canaliculi in rat molars. Anat Embryol (Berl) 1997;195:491-6. https://doi.org/10.1007/s004290050068
  22. Ayasaka N, Kondo T, Goto T, Kido MA, Nagata E, Tanaka T. Differences in the transport systems bet- ween cementocytes and osteocytes in rats using microperoxidase as a tracer. Arch Oral Biol 1992;37: 363-9. https://doi.org/10.1016/0003-9969(92)90019-5
  23. Sasano Y, Maruya Y, Sato H, Zhu JX, Takahashi I, Mizoguchi I, et al. Distinctive expression of extra- cellular matrix molecules at mRNA and protein levels during formation of cellular and acellular cementum in the rat. Histochem J 2001;33:91-9. https://doi.org/10.1023/A:1017948230709
  24. Matsuzawa H, Toriya N, Nakao Y, Konno-Nagasaka M, Arakawa T, Okayama M, et al. Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement. Angle Orthod 2017;87:416-22. https://doi.org/10.2319/071116-541.1
  25. Brudvik P, Rygh P. Root resorption beneath the main hyalinized zone. Eur J Orthod 1994;16:249-63. https://doi.org/10.1093/ejo/16.4.249
  26. Kurol J, Owman-Moll P. Hyalinization and root resorption during early orthodontic tooth movement in adolescents. Angle Orthod 1998;68:161-5.
  27. Kvam E. Scanning electron microscopy of tissue changes on the pressure surface of human premolars following tooth movement. Scand J Dent Res 1972;80:357-68.
  28. Rygh P. Ultrastructural cellular reactions in pressure zones of rat molar periodontium incident to orthodontic tooth movement. Acta Odontol Scand 1972; 30:575-93. https://doi.org/10.3109/00016357209019790

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