Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Mycelial growth characteristics of Sparassis latifolia according to liquid media and incubation conditions

꽃송이버섯 액체종균배지 및 배양조건에 따른 균사 배양 특성

  • Lee, Yun-Hae (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Gwon, Hee-Min (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Gu, Ok (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Choi, Jong-In (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Jeon, Dae-Hoon (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 권희민 (경기도농업기술원 버섯연구소) ;
  • 구옥 (경기도농업기술원 버섯연구소) ;
  • 최종인 (경기도농업기술원 버섯연구소) ;
  • 전대훈 (경기도농업기술원 버섯연구소)
  • Received : 2018.04.24
  • Accepted : 2018.06.25
  • Published : 2018.06.30
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Abstract

Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high ${\beta}$-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.

꽃송이버섯 안정생산을 위해 액체종균배지조성 및 배양조건에 관한 연구결과, 물엿을 8Brix%로 조절하고 질소원으로 Yeast extract를 0.2%첨가한 배지에서 균사생장량이 우수하여 적합 배지로 선발하였다. 통기량에 따른 $CO_2$ 발생량은 배양 9일까지 증가하다 감소하였고, 통기량이 높을수록 균체직경은 작아지고 배지감소율은 증가하여 통기량 0.9vvm이상에서 배지감소율이 급증하여 꽃송이버섯 액체종균에 적합한 통기량은 0.3~0.6 vvm 이 적합하였다. 또한, 배양기간일 길수록 균체량이 많았으나, $CO_2$ 발생량이 높고 배지감소율이 낮은 9~11일을 적합 배양기간으로 설정하였다.

Keywords

References

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