Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Molecular Identification and Chemical Analysis of Aconiti Kusnezoffii Tuber on the Domestic Markets

국내 시장에서 유통되는 초오의 DNA 감별과 화학적 분석

  • Jang, Hyeri (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Joe, Kyeong-Hwa (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Song, Kwangho (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Lee, Kyoung Jin (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Park, Sait Byul (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Lee, Chaemin (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Ha, In Jin (Korean Medicine Clinical Trial Center, Kyung Hee University Korean Medicine Hospital) ;
  • Lee, Kyungjin (Department of Herbology, College of Korean Medicine, Kyung Hee University) ;
  • Suh, Youngbae (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Kim, Yeong Shik (College of Pharmacy, Natural Products Research Institute, Seoul National University)
  • 장혜리 (서울대학교 약학대학 천연물과학연구소) ;
  • 조경화 (서울대학교 약학대학 천연물과학연구소) ;
  • 송광호 (서울대학교 약학대학 천연물과학연구소) ;
  • 이경진 (서울대학교 약학대학 천연물과학연구소) ;
  • 박샛별 (서울대학교 약학대학 천연물과학연구소) ;
  • 이채민 (서울대학교 약학대학 천연물과학연구소) ;
  • 하인진 (경희대학교 경희의료원) ;
  • 이경진 (경희대학교 한의과대학 본초학교실) ;
  • 서영배 (서울대학교 약학대학 천연물과학연구소) ;
  • 김영식 (서울대학교 약학대학 천연물과학연구소)
  • Received : 2018.04.13
  • Accepted : 2018.05.25
  • Published : 2018.06.30
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Abstract

Aconiti Kusnezoffii Tuber has been traditionally used to treat the symptoms of rheumatoid arthritis and joint pain. The main constituents are diterpenoid alkaloids such as benzoylmesaconine, benzoylaconine, mesaconitine, aconitine, and hypaconitine. In Korea, Aconiti Kusnezoffii Tuber is officially defined as the tubers of Aconitum kusnezoffii Reichb., A. ciliare Decasisne, and A. triphyllum Nakai. On the other hand, only the tuber of A. kusnezoffii is to be used in China. In order to identify the botanical origin of Aconiti Kusnezoffii Tuber circulated in Korea, we analyzed 24 samples of Aconiti Kusnezoffii Tuber obtained from local markets for comparative DNA analysis. The sequence analysis of nrRNA ITS 1 was useful to distinguish Aconitum species and revealed that the roots of A. karakolicum were circulated in Korean markets without discretion. HPLC quantitative analysis showed that aconitine was detected at the highest amount in A. karakolicum. Authentic diterpenoid alkaloids were coinjected for quantification of aconitine-type ingredients. All data were statistically grouped by Principal Component Analysis (PCA). This study suggests that both molecular and chemical analyses should be utilized for the standardization and the quality control for Aconiti Kusnezoffii Tuber.

Keywords

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