Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract

트립신 처리에 따른 적송잎 추출물의 항산화 활성 및 항균 효과

  • Moon, Ki-Eun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Park, Kyo-Hyun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Lee, Beom Zoo (CHEMLAND Co., Ltd.) ;
  • Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 문기은 (계명대학교 자연과학대학 공중보건학과) ;
  • 박교현 (계명대학교 자연과학대학 공중보건학과) ;
  • 이범주 ((주)케미랜드) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학과)
  • Received : 2018.03.28
  • Accepted : 2018.06.20
  • Published : 2018.06.28
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Abstract

Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and $ABTS^+$ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.

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References

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