Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitory effects of Synurus excelsus and Weigela subsessilis on aldose reductase and HPLC-UV analysis of scopolin, scopoletin, and quercetin

  • Quilantang, Norman G. (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Ju Sung (Department of Integrative Plant Science, Chung-Ang University) ;
  • Ryu, Seo Hyun (Seoul Science High School) ;
  • Park, Se Hoon (Seoul Science High School) ;
  • Byun, Jae Sang (Seoul Science High School) ;
  • Chun, Je Sung (Seoul Science High School) ;
  • Jacinto, Sonia D. (Institute of Biology, University of the Philippines) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2018.03.22
  • Accepted : 2018.04.14
  • Published : 2018.06.30
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Abstract

The inhibition of aldose reductase (AR) has been shown to prevent the progression of the many complications associated with diabetic hyperglycemia. Several compounds purified from various plant sources have exhibited potent inhibition against AR. In this study, the inhibitory effects of the methanol extracts of the flowers of Synurus excelsus and Weigela subsessilis on AR were determined in vitro. Scopolin and scopoletin are coumarins isolated from the flowers of S. excelsus and W. subsessilis; and quercetin is a known AR inhibitor present in many flowers. To determine and quantify their presence in both plants, HPLC-UV analysis of all three compounds was performed. S. excelsus and W. subsessilis showed potent inhibition against AR having $IC_{50}$ values of 0.17 and $0.14{\mu}g/mL$, respectively. The concentration of scopolin in S. excelsus and W. subsessilis were 34.71 and 174.14 mg/g extract, respectively. Scopoletin was detected in S. excelsus at 3.41 mg/g extract, whereas quercetin was not detected in both plants. This study shows that S. excelsus and W. subsessilis exhibited promising AR inhibitory effects and are both sources of coumarins.

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References

  1. Kador P (1988) The role of aldose reductase in the development of diabetic complications. Med Res Rev 8: 325-352 https://doi.org/10.1002/med.2610080302
  2. Brownlee M (2004) Biology and molecular cell biology of diabetic complications. Diabetes 54: 1615-1625
  3. Robinson Jr. WG, Kador PF, Kinoshita JH (1983) Retinal capillaries: basement membrane thickening by galactosemia prevented with aldose reductase inhibitor. Science 221: 1177-1179 https://doi.org/10.1126/science.6612330
  4. Engerman RL, Kern TS, Larson ME (1994) Nerve conduction and aldose reductase inhibition during 5 years of diabetes or galactosemia in dogs. Diabetologia 37: 141-144 https://doi.org/10.1007/s001250050084
  5. Drel VR, Pacher P, Ali TK, Shin J, Julius U, El-Remessy AB, Obrosova IG (2008) Aldose reductase inhibitor fidarestat counteracts diabetesassociated cataract formation, retinal oxidative-nitrosative stress, glial activation, and apoptosis. Int J Mol Med 21: 667-676
  6. Oates PJ, Mylari BL (1999) Aldose reductase inhibitors: therapeutic implications for diabetic complications. Expert Opin Investig. Drugs 8: 2095-2119 https://doi.org/10.1517/13543784.8.12.2095
  7. Niyeldt R, Van Nood E, Van Hoorn EC, Boelens P, Van Norren K, Van Leeuwen PAM (2001) Flavonoids: a review of probable mechanism of action and potential applications. Am J Clin Nutr 74: 418-425 https://doi.org/10.1093/ajcn/74.4.418
  8. Varman SD, Mikuni I, Kinoshita JH (1975) Flavonoids as inhibitors of lens aldose reductase. Science 188: 1215-1216 https://doi.org/10.1126/science.1145193
  9. Shin HT, Yoo ST, Kim BD, Yi MH (2010) Dispersion of vascular plant in Mt. Huiyangsan, Korea. J Kor Nature 3: 1-10 https://doi.org/10.1016/S1976-8648(14)60001-3
  10. Nam JH, Choi SJ, Lee KR (2004) Phytochemical constituents of Synurus excelsus. Kor J Pharmacogn 35: 116-121
  11. Thuong PT, Na MK, Su ND, Seong RS, Lee YM, Sok DE, Bae KH (2005) Inhibitory effect of coumarins from Weigela subsessilis on low density lipoprotein oxidation. Biol Pharm Bull 28: 1095-1097 https://doi.org/10.1248/bpb.28.1095
  12. Lee J, Ryu H-S, Rodriguez JP, Lee S (2017) Aldose reductase inhibitory activity of quercetin from the stems of Rhododendron mucronulatum for. albiflorum. J Appl Biol Chem 60: 29-33 https://doi.org/10.3839/jabc.2017.006
  13. Hayman S, Kinoshita JJ (1965) Isolation and properties of lens aldose reductase. Biol Chem 240: 877-882
  14. Brownlee M (2001) The pathobiology of diabetic complications: a unifying mechanism. Nature 414: 813-820 https://doi.org/10.1038/414813a
  15. Tang WH, Martin K, Hwa J (2012) Aldose reductase, oxidative stress, and diabetic mellitus. Front Pharmacol 3: 1-8
  16. Grewal AS, Bhardwaj S, Pandita D, Lather V, Sekhon BS (2016) Update on aldose reductase inhibitors for management of diabetic complications and non-diabetic diseases. Mini-Rev Med Chem 16: 120-162
  17. Lee YM, Kim YS, Kim JH, Kim JS (2011) Screening of Korean herbal medicines with inhibitory effect on aldose reductase. Kor J Pharmacogn 42: 161-168
  18. Nazaruk J, Borzym-Kluczyk M (2015) The role of triterpenes in the management of diabetes mellitus and its complications. Phytochem Rev 14: 675-690 https://doi.org/10.1007/s11101-014-9369-x
  19. Lee MS, Lee CM, Cha EY, Thuong PT, Bae KH, Song IS, Noh SM, Sul JY (2010) Activation of AMP-activated protein kinase on human gastric cancer cells by apoptosis induced by corosolic acid isolated from Weigela subsessilis. Phytother Res 24: 1857-1861 https://doi.org/10.1002/ptr.3210
  20. Na M, Thuong PT, Hwang IH, Bae K, Kim BY, Osada H, Ahn S (2010) Protein tyrosinase 1B inhibitory activity of 24-norursane triterpenes isolated from Weigela subsessilis. Phytother Res 24: 1716-1719 https://doi.org/10.1002/ptr.3203
  21. Rao AR, Veeresham C, Asres K (2013) In vitro and in vivo inhibitory activities of four Indian medicinal plant extracts and their major components on rat aldose reductase and the generation of advanced glycation endproducts. Phytother Res 27: 753-760 https://doi.org/10.1002/ptr.4786
  22. Lee K, Yang MC, Lee KH, Choi SY, Lee KR (2007) Phenolic constituents from the flowers of Synurus excelsus. Kor J Pharmacogn 38: 181-186
  23. Pan R, Dai Y, Gao X, Xia Y (2009) Scopolin isolated from Erycibe obtusifolia Benth stems suppresses adjuvant-induced rat arthritis by inhibiting inflammation and angiogenesis. Int J Immunopharmacol 9: 859-869 https://doi.org/10.1016/j.intimp.2009.02.019
  24. Rollinger JM, Hornick A, Langer T, Stuppner H, Prast H (2004) Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products. J Med Chem 47: 6248-6254 https://doi.org/10.1021/jm049655r
  25. Jung HA, Islam MDN, Kwon YS, Jin SE, Son YK, Park JJ, Sohn HS, Choi JS (2011) Extraction and identification of three major aldose reductase inhibitors from Artemisia montana. Food Chem Toxicol 49: 376-384 https://doi.org/10.1016/j.fct.2010.11.012

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