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Chemical Composition and Antioxidant Activity of Algerian Juniperus Phoenicea Essential Oil

  • Harhour, Aicha (Laboratory of Natural Substances Valorization, University of Djilali Bounaama) ;
  • Brada, Moussa (Laboratory of Natural Substances Valorization, University of Djilali Bounaama) ;
  • Fauconnier, Marie-Laure (Agro-Bio Chem Department, General and Organic Chemistry, University of Liege, Gembloux Agro-Bio Tech) ;
  • Lognay, Georges (Agro-Bio Chem Department, Analytical Chemistry, University of Liege, Gembloux Agro-Bio Tech)
  • Received : 2018.02.15
  • Accepted : 2018.03.29
  • Published : 2018.06.30

Abstract

Berries and branches essential oil of Juniperus phoenicea were obtained by electromagnetic induction heating assisted extraction and by hydrodistillation with a yield varied from ($1.2{\pm}0.3$ to $2.4{\pm}0.7%$) and from ($0.6{\pm}0.1%$ to $1.1{\pm}0.1%$), respectively. forty eight compounds were identified representing (97.2 - 99.7%) of the oil. ${\alpha}$-Pinene (40.3 - 67.8%) and ${\delta}$-3-carene (13.5 - 26.8%) were the main compounds in berries and branches essential oils. Antioxidant activity was evaluated by three means: inhibition of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical, reducing power and ${\beta}$-Carotene/linoleic acid bleaching. The antioxidant activity of essential oils showed $IC_{50}$ ranging from $67.6{\pm}1.02{\mu}g/mL$ to $131.5{\pm}0.8{\mu}g/mL$ for berries and from $98{\pm}1.25{\mu}g/mL$ to $166.8{\pm}0.29{\mu}g/mL$ for the branches. Berries oil show more potent antioxidant activity compared to branches. This result is supported by the three methods investigated in this work.

Keywords

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