Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Lactobacillus salivarius on growth performance, diarrhea incidence, fecal bacterial population and intestinal morphology of suckling pigs challenged with F4+ enterotoxigenic Escherichia coli

  • Sayan, Harutai (Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Assavacheep, Pornchalit (Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Angkanaporn, Kris (Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Assavacheep, Anongnart (Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University)
  • Received : 2017.10.10
  • Accepted : 2018.03.13
  • Published : 2018.08.01
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Abstract

Objective: Gut health improvements were monitored with respect to growth performance, diarrhea incidence, fecal bacterial population and intestinal morphology of suckling pigs orally supplemented with live Lactobacillus salivarius (L. salivarius) oral suspensions and challenged with $F4^+$ enterotoxigenic Escherichia coli (ETEC). Methods: Two groups of newborn pigs from 18 multiparous sows were randomly designated as non-supplemented (control: n = 114 piglets) and L. salivarius supplemented groups (treatment: n = 87 piglets). Treatment pigs were orally administered with 2 mL of $10^9$ colony-forming unit (CFU)/mL L. salivarius on days 1 to 3, then they were orally administered with 5 mL of $10^9CFU/mL$ L. salivarius on days 4 to 10, while those in control group received an equal amount of phosphate buffered saline solution. On day 24 (2 weeks post supplementation), one pig per replicate of both groups was orally administered with $10^8CFU/mL$ $F4^+$ ETEC, then they were euthanized on day 29 of experiment. Results: Results revealed that pigs in treatment group had a statistically significant increase in average daily gain, body weight and weight gain, and tended to lower diarrhea throughout the study. Numbers of Lactobacillus population in feces of treatment pigs were higher than control pigs, especially on day 10 of study. Numbers of total bacteria in intestinal contents of control pigs were also increased, but not Coliform and Lactobacillus populations. Histological examination revealed statistically significant improvements of villous height and villous/crypt ratio of duodenum, proximal jejunum and distal jejunum parts of treatment pigs compared with controls. Duodenal pH of treatment group was significantly decreased. Conclusion: Oral supplementation of live L. salivarius during the first 10 days of suckling pig promoted growth performance and gut health, reduced diarrhea incidence, increased fecal Lactobacillus populations and improved intestinal morphology.

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References

  1. Chan G, Farzan A, DeLay J, et al. A retrospective study on the etiological diagnoses of diarrhea in neonatal piglets in Ontario, Canada, between 2001 and 2010. Can J Vet Res 2013;77:254-60.
  2. Begum S, Patra MK, Ngullie L, et al. Antimicrobial resistance pattern in swine colibacillosis under farm and field condition in Nagaland. Int J Livest Res 2014;4:81-8.
  3. Chandler D, Mynott T. Bromelain protects piglets from diarrhoea caused by oral challenge with K88 positive enterotoxigenic Escherichia coli. Gut 1998;43:196-202. https://doi.org/10.1136/gut.43.2.196
  4. Estienne M, Hartsock T, Harper A. Effects of antibiotics and probiotics on suckling pig and weaned pig performance. Intern J Appl Res Vet Med 2005;3:303-8.
  5. Moxley RA, Duhamel GE. Comparative pathology of bacterial enteric disease of swine. Adv Exp Med Biol 1999;473:83-101.
  6. Salih N, Hutari A, Gaseem W, et al. Maximization of growth and storage of locally isolated Lactobacillus salivarius subsp. Salivarius with high stability and functionality. In: Proceedings of Southern Biomedical Engineering 2009; 2009 May 15-17, Miami, FL, USA: Springer-Verlag Berlin heidelberg; 2009. p. 175-8.
  7. Meng QW, Yan L, Ao X, et al. Influence of probiotics in different energy and nutrient density diets on growth performance, nutrient digestibility, meat quality, and blood characteristics in growing-finishing pigs. J Anim Sci 2010;88:3320-6. https://doi.org/10.2527/jas.2009-2308
  8. Vasala A, Panula J, Neubauer P. Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganisms. J Biotechnol 2005;117:421-31. https://doi.org/10.1016/j.jbiotec.2005.02.010
  9. Latimer GW. AOAC International. Official methods of analysis of AOAC International. 19th ed. Gaithersburg, MD, USA: AOAC International; 2012.
  10. Bacteriological analytical manual online [Internet]. Food and Drug Administration; 2001 [cited 2014 Mar 18]. Available from: https://www.epa.gov/sites/production/files/2015-07/documents/fda-bam-appendix1.pdf
  11. Robb EJ, Bryson WL, Chester ST, et al. Efficacy of ceftiofur sodium for the control of mortality in neonatal pigs orally inoculated with K88 (F4) enterotoxigenic Escherichia coli. J Swine Health Prod 2003;11:7-11.
  12. Giang HH, Viet TQ, Ogle B, et al. Growth performance, digestibility, gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria. Livest Sci 2010;129:95-103. https://doi.org/10.1016/j.livsci.2010.01.010
  13. Liu P, Piao X, Thacker P, et al. Chito-oligosaccharide reduces diarrhea incidence and attenuates the immune response of weaned pigs challenged with K88. J Anim Sci 2010;88:3871-9. https://doi.org/10.2527/jas.2009-2771
  14. Tsirtsikos P, Fegeros K, Kominakis A, et al. Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers. Animal 2012;6:1049-57. https://doi.org/10.1017/S1751731111002680
  15. SAS. SAS/SAT guide for personal computers. 9.00ed. Carry, NC, USA: SAS Inst., Inc.; 2002.
  16. Zhang J, Deng J, Wang C, et al. Modulatory effects of Lactobacillus salivarius on intestinal mucosal immunity of piglets. Curr Microbiol 2011;62:1623-31. https://doi.org/10.1007/s00284-011-9906-4
  17. Banach S, Rehberger T, Parrott T, et al. Influence of Lactobacillus brevis strain 1E-1 on the gastrointestinal microflora of preweaning and weaning pigs. In: American Society of Animal Science Meeting 2002; 2002 Mach 18-20; Iowa, USA.
  18. Rondon AJ, OjitoY, Arteaga FG, et al. Probiotic effect of Lactobacillus salivaruis C 65 on productive and health indicators of lactating piglets. Cuban J Agric Sci 2013;47:401-7.
  19. Yu H, Wang A, Li X, et al. Effect of viable Lactobacillus fermentum on the growth performance, nutrient digestibility and immunity of weaned pigs. J Anim Feed Sci 2008;17:61-9. https://doi.org/10.22358/jafs/66470/2008
  20. Ammor S, Tauveron G, Dufour E, et al. Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: 1-Screening and characterization of the antibacterial compounds. Food Control 2006;17:454-61. https://doi.org/10.1016/j.foodcont.2005.02.006
  21. Huang C, Qiao S, Li D, et al. Effects of lactobacilli on the performance, diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian-Australas J Anim Sci 2004;17:401-9. https://doi.org/10.5713/ajas.2004.401
  22. Yang KM, Jiang ZY, Zheng CT, et al. Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88. J Anim Sci 2014;92:1496-503. https://doi.org/10.2527/jas.2013-6619
  23. Miriam BB, Julio PD, Sergio MQ, et al. Probiotic mechanisms of action. Ann Nutr Metab 2012;61:160-74. https://doi.org/10.1159/000342079
  24. Lahteinen T, Malinen E, Koort JM, et al. Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe 2010;16:293-300. https://doi.org/10.1016/j.anaerobe.2009.08.002
  25. Zhang L, Xu YQ, Liu HY, et al. Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea: Effects on diarrhoea incidence, faecal microflora and immune responses. Vet Microbiol 2010;141:142-8. https://doi.org/10.1016/j.vetmic.2009.09.003
  26. Lahteinen T, Rinttila T, Koort JM, et al. Effect of a multispecies lactobacillus formulation as a feeding supplement on the performance and immune function of piglets. Livest Sci 2015;180:164-71. https://doi.org/10.1016/j.livsci.2015.07.016
  27. Suo C, Yin Y, Wang X, et al. Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality. BMC Vet Res 2012;8:89. https://doi.org/10.1186/1746-6148-8-89
  28. Maria MR, Deise OS, Ernesto AT, et al. IgA production, coliforms analysis and intestinal mucosa IgA production, coliforms analysis and intestinal mucosa morphology of piglets that received probiotics with viable or morphology of piglets that received probiotics with viable or inactivated cells inactivated cells. Pesq Vet Bras 2007;27:241-5. https://doi.org/10.1590/S0100-736X2007000600004

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