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The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment

  • Law, Fang Lin (Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia) ;
  • Zulkifli, Idrus (Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia) ;
  • Soleimani, Abdoreza Farjam (Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia) ;
  • Liang, Juan Boo (Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia) ;
  • Awad, Elmutaz Atta (Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia)
  • 투고 : 2017.08.05
  • 심사 : 2017.11.04
  • 발행 : 2018.08.01

초록

Objective: This experiment was conducted to investigate the effects of dietary crude protein (CP) level and exogenous protease supplementation on growth performance, serum metabolites, carcass traits, small intestinal morphology and endogenous protease activity in broiler chickens reared under a tropical climate. Methods: A total of 480 day-old male broiler chicks were randomly assigned to eight dietary treatments in a $4{\times}2$ factorial arrangement. The main effects were CP level (21.0%, 19.7%, 18.5%, or 17.2% from 1 to 21 days and 19.0%, 17.9%, 16.7%, or 15.6% from 22 to 35 days) and protease enzyme supplementation (0 ppm or 500 ppm). All experimental diets were fortified with synthetic feed-grade lysine, methionine, threonine and tryptophan to provide the minimum amino acid recommended levels for Cobb 500. Results: Reducing dietary CP linearly reduced (p<0.05) growth performance, serum albumin, total protein, and carcass traits and increased (p<0.05) serum triglycerides and abdominal fat. There was no consistent effect of reducing dietary CP on morphological parameters of the intestine and on the pancreatic and intestinal endogenous protease activity (p>0.05). Protease supplementation improved (p<0.05) feed conversion ratio, body weight gain, carcass yield and intestinal absorptive surface area. Conclusion: Protease supplementation, as measured by growth performance, intestinal morphology and carcass yield, may alleviate the detrimental effects of low protein diets in broiler chickens.

키워드

참고문헌

  1. Bregendahl K, Sell JL, Zimmerman DR. Effect of low protein diets on growth performance and body composition of broiler chicks. Poult Sci 2002;81:1156-67. https://doi.org/10.1093/ps/81.8.1156
  2. Waldroup PW, Jiang Q, Fritts C. Effects of supplementing broiler diets low in crude protein with essential and nonessential amino acids. Poult Sci 2005;4:425-31. https://doi.org/10.3923/ijps.2005.425.431
  3. Namroud NF, Shivazad M, Zaghari M. Effects of fortifying low crude protein diet with crystalline amino acids on performance, blood ammonia level, and excreta characteristics of broiler chicks. Poult Sci 2008;87:2250-8. https://doi.org/10.3382/ps.2007-00499
  4. Laudadio V, Passantino L, Perillo A, et al. Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poult Sci 2012;91:265-70. https://doi.org/10.3382/ps.2011-01675
  5. Awad EA, Zulkifli I, Soleimani AF, Loh TC. Amino acids fortification of low-protein diet for broilers under tropical climate. 2. Nonessential amino acids and increasing essential amino acids. Italian J Anim Sci 2014;13:631-6.
  6. Awad EA, Zulkifli I, Soleimani AF, Aljuobori A. Effects of feeding male and female broiler chickens on low-protein diets fortified with different dietary glycine levels under the hot and humid tropical climate. Italian J Anim Sci 2017;16:453-61. https://doi.org/10.1080/1828051X.2017.1291288
  7. Temim S, Chagneau AM, Guillaumin S, et al. Effects of chronic heat exposure and protein intake on growth performance, nitrogen retention and muscle development in broiler chickens. Reprod Nutr Dev 1999;39:145-56. https://doi.org/10.1051/rnd:19990147
  8. Cowieson AJ, Ravindran V. Effect of exogenous enzymes in maize based diets varying in nutrient density for young broilers: growth performance and digestibility of energy, minerals and amino acids. Br Poult Sci 2008;49:37-44. https://doi.org/10.1080/00071660701812989
  9. Ding XM, Li DD, Li ZR, et al. Effects of dietary crude protein levels and exogenous protease on performance, nutrient digestibility, trypsin activity and intestinal morphology in broilers. Livest Sci 2016;193:26-31. https://doi.org/10.1016/j.livsci.2016.09.002
  10. Mahmood T, Mirza M, Nawaz H, et al. Effect of supplementing exogenous protease in low protein poultry by-product meal based diets on growth performance and nutrient digestibility in broilers. Anim Feed Sci Technol 2017;228:23-31. https://doi.org/10.1016/j.anifeedsci.2017.01.012
  11. Angel C, Saylor W, Vieira S, Ward N. Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens. Poult Sci 2011;90:2281-6. https://doi.org/10.3382/ps.2011-01482
  12. Law FL, Zulkifli I, Soleimani AF, Hossain M, Liang JB. Nutrient digestibility of broiler chickens fed on a low-protein diet supplemented with mono-component proteases. Eur Poult Sci 2015;79:107.
  13. Mahagna M, Nir I, Larbier M, Nitsan Z. Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks. Reprod Nutr Dev1995;35:201-12. https://doi.org/10.1051/rnd:19950208
  14. Yegani M, Korver DR. Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens. Poult Sci 2013;92:1208-20. https://doi.org/10.3382/ps.2012-02390
  15. Cowieson A, Zaefarian F, Knap I, Ravindran VR. Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health. Anim Produ Sci 2016;57:1058-68.
  16. Kamel NF, Ragaa NM, El-Banna RA, Mohamed FF. Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens. Agric Agric Sci Procedia 2015;6:216-25. https://doi.org/10.1016/j.aaspro.2015.08.062
  17. Xu X, Wang HL, Pan L, et al. Effects of coated proteases on the performance, nutrient retention, gut morphology and carcass traits of broilers fed corn or sorghum based diets supplemented with soybean meal. Anim Feed Sci Technol 2017;223:119-27. https://doi.org/10.1016/j.anifeedsci.2016.10.015
  18. Marchini CFP, Cafe MB, Araujo EG, Nascimento MRBM. Physiology, cell dynamics of small intestinal mucosa, and performance of broiler chickens under heat stress: a review. Rev Colom Cienc Pecua 2016;29:159-68.
  19. Jin L, Ho Y, Abdullah N, Jalaludin S. Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poult Sci 2000;79:886-91. https://doi.org/10.1093/ps/79.6.886
  20. AOAC (Association of Official Analytical Chemists) international. Official methods of analysis. 17th ed. Association of Official Analytical Chemist. Arlington, VA, USA: AOAC International; 2000.
  21. Sklan D, Plavnik I. Interactions between dietary crude protein and essential amino acid intake on performance in broilers. Br Poult Sci 2002;43:442-9. https://doi.org/10.1080/00071660120103710
  22. Rehman Z, Kamran J, El-Hack MA, et al. Influence of lowprotein and low-amino acid diets with different sources of protease on performance, carcasses and nitrogen retention of broiler chickens. Anim Prod Sci 2017 Mar 30 [Epub]. https://doi.org/10.1071/AN16687
  23. Lauri A, Hanauska-Brown, Alfred M, Dufty JR. Blood chemistry, cytology and body condition in adult northern goshawks. J Raptor Res 2003;37:299-306.
  24. Abudabos AM. Effect of enzyme supplementation to normal and low density broiler diets based on corn-soybean meal. Asian J Anim Vet Adv 2012;7:139-48. https://doi.org/10.3923/ajava.2012.139.148
  25. Lensing M, Van Der Klis J, Le bellego L, Rovers M. The threonine requirement of broiler chickens during subclinical intestinal infection. In: Proceedings of the 16th European Symposium on Poultry Nutrition 2007; 2007 August 26-30; Strasbourg, France: World Poultry Science Association; 2007. p. 343-6.
  26. Ghazi S, Rooke JA, Galbraith H, Bedford MR. The potential for the improvement in the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels. Br Poult Sci 2002;43:70-7. https://doi.org/10.1080/00071660120109935

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  1. Meeting Global Feed Protein Demand: Challenge, Opportunity, and Strategy vol.7, pp.1, 2019, https://doi.org/10.1146/annurev-animal-030117-014838
  2. Effects of protease supplementation of low protein and/or energy diets on growth performance and blood parameters in broiler chickens under heat stress condition pp.1828-051X, 2019, https://doi.org/10.1080/1828051X.2018.1557019
  3. Effects of Low-Protein Diets and Exogenous Protease on Growth Performance, Carcass Traits, Intestinal Morphology, Cecal Volatile Fatty Acids and Serum Parameters in Broilers vol.9, pp.5, 2019, https://doi.org/10.3390/ani9050226
  4. Response of broilers to reduced-protein diets under heat stress conditions vol.75, pp.4, 2019, https://doi.org/10.1017/s0043933919000576
  5. Effect of Bacillus pumilus 3-19 protease on growth parameters and gut microbiome of broiler chickens vol.222, pp.None, 2018, https://doi.org/10.1051/e3sconf/202022202051
  6. Effect of Supplemental Protease on Growth Performance and Excreta Microbiome of Broiler Chicks vol.8, pp.4, 2020, https://doi.org/10.3390/microorganisms8040475
  7. The Response of Broiler Chickens to Dietary Soybean Meal Reduction with Glycine and Cysteine Inclusion at Marginal Sulfur Amino Acids (SAA) Deficiency vol.10, pp.9, 2018, https://doi.org/10.3390/ani10091686
  8. Amino acid digestibility coefficient values of animal protein meals with dietary protease for broiler chickens vol.4, pp.4, 2020, https://doi.org/10.1093/tas/txaa187
  9. Response of broilers to gradual dietary protein reduction with or without an adequate glycine plus serine level vol.19, pp.1, 2018, https://doi.org/10.1080/1828051x.2019.1704634
  10. Passage time, apparent metabolisable energy and ileal amino acids digestibility of treated palm kernel cake in broilers under the hot and humid tropical climate vol.19, pp.1, 2018, https://doi.org/10.1080/1828051x.2020.1712266
  11. Effects of gelatin as an alternative protein source and mono-component protease supplementation on growth performance, viscosity, digestibility and microbial population of ileal digesta, digestive tra vol.244, pp.None, 2021, https://doi.org/10.1016/j.livsci.2020.104326
  12. Effect of Low Protein Diets with Amino Acids Supplementation on Growth Performance, Carcass Traits, Blood Parameters and Muscle Amino Acids Profile in Broiler Chickens under High Ambient Temperature vol.11, pp.2, 2021, https://doi.org/10.3390/agriculture11020185
  13. Visceral organ weight of broiler chicken fed different level protein and protease enzyme supplementation diet vol.788, pp.1, 2018, https://doi.org/10.1088/1755-1315/788/1/012032
  14. The importance of nutrition in preventing heat stress at poultry vol.77, pp.3, 2018, https://doi.org/10.1080/00439339.2021.1938340
  15. A review of limitations to using cassava meal in poultry diets and the potential role of exogenous microbial enzymes vol.53, pp.4, 2018, https://doi.org/10.1007/s11250-021-02853-6
  16. Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15-28 Days vol.11, pp.9, 2018, https://doi.org/10.3390/ani11092499
  17. Interactive effect of exogenous protease enzyme and dietary crude protein levels on growth and digestibility indices in broiler chickens during the starter phase vol.53, pp.1, 2021, https://doi.org/10.1007/s11250-020-02466-5
  18. Heterologous Expression of Bacillus pumilus 3-19 Protease in Pichia pastoris and Its Potential Use as a Feed Additive in Poultry Farming vol.11, pp.4, 2018, https://doi.org/10.1007/s12668-021-00899-2
  19. Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens vol.17, pp.1, 2021, https://doi.org/10.1186/s12917-021-02946-2