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표고 야생형 계통의 배양 및 형태적 특성 비교

Cultural Characteristics and Morphological Comparison of the Wild Mushroom Lentinula edodes Cultivated on Sawdust Substrate

  • 박영애 (국립산림과학원 산림소득자원연구과) ;
  • 서수영 (국립산림과학원 산림소득자원연구과) ;
  • 가강현 (국립산림과학원 산림소득자원연구과)
  • Park, Young-Ae (Special Forest Products Division, National Institute of Forest Science) ;
  • Seo, Sooyoung (Special Forest Products Division, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Special Forest Products Division, National Institute of Forest Science)
  • 투고 : 2018.04.13
  • 심사 : 2018.05.28
  • 발행 : 2018.06.01

초록

야생 표고 26개 균주를 육종의 소재로 활용할 목적으로 배양 특성 및 버섯 생산성과 자실체의 특성 등을 조사하였다. potato dextrose agar 배지에서 균사의 배양 온도($10^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$)에 따른 균사 생장력을 측정한 결과, 3가지 온도 조건에서 공통적으로 평균 이상의 생장력을 갖는 균주는 NIFoS 68, NIFoS 136, NIFoS 1521, NIFoS 1651, NIFoS 2064였다. NIFoS 411 균주는 $20^{\circ}C$에서 가장 저조한 생장을 보인 반면 $30^{\circ}C$에서 가장 높은 생장을 보였다. 배양 배지의 중량 감소율은 13.5~47.5%로 NIFoS 50 (47.5%), NIFoS 128 (34.5%), NIFoS 54 (34.4%) 순으로 조사되었다. 또한 26개 균주 중 24개 균주에서 버섯이 발생되었고, 생산성은 3~446 g/2 kg이었다. 버섯 생산량과 온도별 균사 생장력은 크게 상관관계를 보이지 않았으나 중량 감소율이 클수록 자실체 수확량이 높은 상관관계를 보였다. 생산량 측면을 고려할 때, 3개 균주 NIFoS 50 (446 g), NIFoS 53 (421 g), NIFoS 952 (435 g)은 육종 소재로 활용 가능하였고, 형태적인 면을 고려할 때 NIFoS 667 균주가 우수하였으며, NIFoS 670 균주는 자실체의 갓이 황갈색인 특징을 보였다.

The cultivation characteristics of 26 wild strains of Lentinula edodes were investigated for their use as breeding material. Strains NIFoS 68, 136, 1521, 1651, and 2064 showed an above average mycelial growth on potato dextrose agar at 10, 20, and $30^{\circ}C$. NIFoS 411 showed the lowest mycelial growth at $20^{\circ}C$, but the highest growth at $30^{\circ}C$. The rate of weight loss of L. edodes cultivated on sawdust (2 kg) ranged from 13.5 to 47.5%, with the highest rates showed by NIFoS 50 (47.5%), NIFoS 128 (34.5%), and NIFoS 54 (34.4%). Fruiting bodies were produced in nearly all (24/26) strains and productivity ranged from 3 g to 446 g/2 kg medium. Temperature was not significantly correlated with mushroom production or mycelial growth. Larger weight loss correlated strongly with fruit yield. In terms of production, NIFoS 50 (446 g), NIFoS 952 (435 g), and NIFoS 53 (421 g) were useful as breeding material. The NIFoS 667 strain was superior in terms of morphology. NIFoS 670 showed the characteristic yellowish-brown color of fruiting bodies.

키워드

참고문헌

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