The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

First Report on Four Novel Endophytic Fungal Species Isolated from Leaves of Juniperus chinensis var. sargentii in Korea

눈향나무의 잎에서 분리된 4종의 국내 미기록 내생균

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2018.05.16
  • Accepted : 2018.05.25
  • Published : 2018.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated endophytic fungi from the leaves of the conifer Juniperus chinensis var. sargentii inhabiting Mt. Hallasan. The fungal isolates were identified based on phylogenetic analysis using nucleotide sequences of the internal transcribed spacer region, large subunit region of ribosomal DNA, translation elongation factor, and ${\beta}$-tubulin region. In this study, we confirmed four species of unreported endophytic fungi in Korea, Allantophomopsis lycopodina, Epicoleosporium ramularioides, Kabatina juniperi, and Seiridium podocarpi. We describe their morphological characteristics and the results of the phylogenetic analysis.

한라산에 서식하는 눈향나무의 침엽에서 내생균을 분리하였다. 분리된 균주들은 형태적 특성과 internal transcribed spacer 영역, large subunit rDNA 영역, translation elongation factor 영역 및 ${\beta}$-tublin 영역 염기서열의 계통분석을 이용하여 동정하였다. 그 결과 국내 미기록 내생균인 Allantophomopsis lycopodina, Epicoleosporium ramularioides, Kabatina juniperi, Seiridium podocarpi 4종의 균주를 확인하였다. 4종의 미기록 균주에 대한 형태적 특성 및 계통분석의 결과에 대해 기술하였다.

Keywords

References

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Schulz B, Boyle C, Draeger S, Rommert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004. https://doi.org/10.1017/S0953756202006342
  3. Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep 2001;18:448-59. https://doi.org/10.1039/b100918o
  4. Hata K, Futai K, Tsuda M. Seasonal and needle age-dependent changes of the endophytic mycobiota in Pinus thunbergii and Pinus densiflora needles. Can J Bot 1998;76:245-50.
  5. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes‐ application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  6. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1093/sysbio/49.2.278
  7. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323-30.
  8. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-${\alpha}$ sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005;97:84-98.
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  10. Videira SI, Groenewald JZ, Braun U, Shin HD, Crous PW. All that glitters is not Ramularia. Stud Mycol 2016;83:49-163. https://doi.org/10.1016/j.simyco.2016.06.001
  11. Schneider R, von Arx JA. Two new fungi causing twig dieback: Kabatina thujae gen. et sp. nov. and K. juniperi sp. nov. J Phytopathol 1966;57:176-82. https://doi.org/10.1111/j.1439-0434.1966.tb04722.x
  12. Petrini O, Carroll G. Endophytic fungi in foliage of some Cupressaceae in Oregon. Can J Bot 1981;59:629-36. https://doi.org/10.1139/b81-089
  13. Carris LM. Cranberry black rot fungi: Allantophomopsis cytisporea and Allantophomopsis lycopodina. Can J Bot 1990;68:2283-91. https://doi.org/10.1139/b90-291
  14. Debbab A, Aly AH, Proksch P. Bioactive secondary metabolites from endophytes and associated marine derived fungi. Fungal Divers 2011;49:1. https://doi.org/10.1007/s13225-011-0114-0
  15. Shiono Y, Yokoi M, Koseki T, Murayama T, Aburai N, Kimura K. Allantopyrone A, a new ${\alpha}$-pyrone metabolite with potent cytotoxicity from an endophytic fungus, Allantophomopsis lycopodina KS-97. J Antibiot 2010;63:251-3. https://doi.org/10.1038/ja.2010.18
  16. Crous PW, Shivas RG, Quaedvlieg W, van der Bank M, Zhang Y, Summerell BA, Guarro J, Wingfield MJ, Wood AR, Alfenas AC, et al. Fungal Planet description sheets: 214-280. Persoonia 2014;32:184-306. https://doi.org/10.3767/003158514X682395