The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Characterization of Wild Yeasts from Water and Riverside Soils of Geumgang Midstream in Gongju City, Korea

금강 중류 공주시 주변 물과 토양으로부터 야생효모의 분리 및 국내 미기록 효모 특성

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 김지윤 (배재대학교 바이오.의생명공학과) ;
  • 이향범 (전남대학교 농식품생명화학부) ;
  • 김하근 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2018.04.10
  • Accepted : 2018.05.08
  • Published : 2018.06.01
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Abstract

The goal of this study was to elucidate wild yeast diversity of the midstream region of the Geumgang river, Gongju, Chungnam province, Korea. Fifty strains of 24 species of wild yeasts were isolated from 45 water and soil samples among which Cryptococcus spp. (11 strains), Cryptococcus magnus (7 strains), Rhodotorula spp. (9 strains), and Hanseniaspora spp. (6 strains) were dominant. Four species, Candida chauliodes WJSF 0201, Candida oleophila WJSF 0202, Candida catenulata WJSF 0203, and Candida jaroonii WJSF 0204, represented newly recorded yeasts in Korea. All of these unrecorded yeasts were oval in shape, formed ascospores and pseudomycelium, and grew in vitamin-free medium. Candida oleophila WJSF 0202 was thermophilic which can grow at $37^{\circ}C$.

우리나라 3대 강의 하나인 금강의 물과 주변 토양들의 야생효모들의 분포 특성을 알아보기 위해 먼저 금강 중류지역인 공주시 청벽대교 밑을 흐르는 금강의 물과 주변 토양들을 2017년 10월에 45점을 채취하여 야생효모들을 분리, 동정하였고 이들 중 국내 미기록 효모들을 선별하여 이들의 균학적 특성을 조사하였다. 이들 금강 중류 시료들로부터 24종 51균주가 분리되었고 이들 중 가장 많이 분리된 야생효모는 Cryptococcus magnus 7주를 포함한 Cryptococcus속 균으로 11주가 분리되었고 Rhodotorula속 균과 Hanseniaspora속 균들이 우점균으로 많이 분리 되었다. Candida chauliodes WJSF 0201, Candida oleophila WJSF 0202, Candida catenulata WJSF 0203, Candida jaroonii WJSF 0204 등 4균주들이 국내 미기록 효모 균주들로 선별되었고 이들 야생효모들은 구형으로 출아법으로 영양증식하였다. 모든 미기록 효모 균주들이 비타민을 함유하지 않은 yeast extract-peptone-dextrose (YPD) 배지에서도 잘 생육하였고 5% NaCl를 함유한 YPD 배지에서 생육하는 내염성 효모들이었다. 특히, Candida oleophila WJSF 0202는 $37^{\circ}C$에서도 생육하는 고온성 효모로 내열성 효소 등 산업적으로 매우 유용할 것으로 생각된다.

Keywords

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