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Calendar for the Prevalence of Honey Bee Diseases, with Studying the Role of Some Materials to Control Nosema

몇 가지 꿀벌 노제마병 방제물질의 평가와 꿀벌 질병의 발생시기 조사

  • Received : 2018.02.01
  • Accepted : 2018.05.04
  • Published : 2018.06.01

Abstract

Nosema spp. (NS) causes severe problems to honey bee colonies including the death of the highly infected ones. Searching for effective materials to control this parasite is very important. The objectives of this study were to identify the calendar for the prevalence of NS and other bee diseases, and to test the efficacy of three materials: diluted honey mixed with lemon juice (M1), chamomile extract mixed with sugar syrup (M2) and sutrivet mixed with sugar syrup (M3) against Nosema. To realize these objectives, diseases of brood and adult honey bees were surveyed over one year. Also, the efficacy of M1, M2 and M3 against Nosema was evaluated under field and laboratory conditions. The results showed that few diseases for immature and mature stages of honey bees were recorded. NS was detected during winter and spring in link with low temperature and high relative humidity. Under field conditions, M2 reduced the infection by 36.66% while M3 by 23.33% and finally M1 by 13.33%. In the laboratory, the highest efficacy was to M2 followed by M1 and finally M3. The three materials impacted the percentage of survived bees significantly higher than infected bees without any treatments over the experimental period. The study suggests the potential role of chamomile as a natural material to control NS.

미포장충류(Nosema spp. (NS))는 양봉꿀벌에 심각한 문제를 야기시키는 기생충으로 효과적인 방제물질의 선발이 무엇보다 중요하다. 본 연구는 노제마병과 기타 꿀벌의 발생유행시기의 구명과 더불어 3가지 노제마병 방제물질(M1 = 벌꿀희석의 레몬쥬스; M2 = 설탕시럽 혼합의 카모마일 추출물; M3 = 설탕시럽 혼합의 항생물질 스트리베트)을 평가하고자 수행하였다. 꿀벌 성충과 유충집단의 질병 유행시기를 년간 조사하였으며, 야외 및 실험실 조건에서 노제마병에 대한 M1, M2, M3의 효과를 평가하였다. 조사결과 극소수의 꿀벌 성충과 유충 질병이 발견되었다. 노제마병은 겨울과 봄 기간 저온과 고습조건에서 검출되었다. 포장실험에서 M2는 36.66%까지 발병억제 능력을 보였으며, 반면M3는 23.33%, M1는 13.33%의 억제효과를 보였다. 실내실험에서 M2가 방제효과가 가장 좋았고, 그 다음 M1와 M3 이었다. 3가지 방제물질은 병에 감염된 꿀벌성충의 생존력을 크게 높이는 것으로 나타났다. 본 연구는 노제마병 방제를 위한 천연물질로 카모마일의 잠재적 방제효과를 제시하고 있다.

Keywords

References

  1. Abd-El-Samie, E.M., Adham, F.K., El-Mohandes, S., Seyam, H., 2017. First detection of deformed wing and kakugo viruses in honeybees (Apis mellifera L.) in Egypt by real time-polymerase chain reaction (RT-PCR). Afr. J. Biotechnol. 16, 738-748. https://doi.org/10.5897/AJB2016.15557
  2. Alaux, C., Folschweiller, M., McDonnell, C., Beslay, D., Cousin, M., Dussaubat, C., Brunet, J.-L., Le Conte, Y., 2011. Pathological effects of the microsporidium Nosema ceranae on honey bee queen physiology (Apis mellifera). J. Invertebr. Pathol. 106, 380-385. https://doi.org/10.1016/j.jip.2010.12.005
  3. Aly, M.Z.Y., Mohanny, K.M., Mohamedain, H.S., Elnasr, R.A.S., 2012. The relationship between the infection levels of Nosema apis parasites and epithelial cells of mid-gut of honeybee in Qena Government. Egypt. Acad. J. biolog. Sci., 3, 1-11.
  4. Ansari, M.J., Al-Ghamdi, A., Nuru, A., Khan, K.A., Alattal, Y., 2017. Geographical distribution and molecular detection of Nosema ceranae from indigenous honey bees of Saudi Arabia. Saudi J. Biol. Sci. 24, 983-991. https://doi.org/10.1016/j.sjbs.2017.01.054
  5. Antunez, K., Martin-Hernandez, R., Prieto, L., Meana, A., Zunino, P., Higes, M., 2009. Immune suppression in the honey bee (Apis mellifera) following infection by Nosema ceranae (Microsporidia). Environ. Microbiol. 11, 2284-2290. https://doi.org/10.1111/j.1462-2920.2009.01953.x
  6. Borsuk, G., Ptaszynska, A.A., Olszewski, K., Paleolog, J., 2013. Impact of nosemosis on the intestinal yeast flora of honey bees. Med. Weter. 69, 726-729.
  7. Botias, C., Martin-Hernandez, R., Meana, A., Higes, M., 2013. Screening alternative therapies to control Nosemosis type C in honey bee (Apis mellifera iberiensis) colonies. Res. Vet. Sci. 95, 1041-1045. https://doi.org/10.1016/j.rvsc.2013.09.012
  8. Chen, Y.-W., Chung, W.-P., Wang, C.-H., Solter, L.F., Huang, W.-F., 2012. Nosema ceranae infection intensity highly correlates with temperature. J. Invertebr. Pathol. 111, 264-267. https://doi.org/10.1016/j.jip.2012.08.014
  9. Costa, C., Lodesani, M., Maistrello, L., 2010. Effect of thymol and resveratrol administered with candy or syrup on the development of Nosema ceranae and on the longevity of honeybees (Apis mellifera L.) in laboratory conditions. Apidologie 41, 141-150. https://doi.org/10.1051/apido/2009070
  10. De Miranda, J.R., Genersch, E., 2010. Deformed wing virus. J. Invertebr. Pathol. 103, S48-S61. https://doi.org/10.1016/j.jip.2009.06.012
  11. Dussaubat, C., Maisonnasse, A., Alaux, C., Tchamitchan, S., Brunet, J.-L., Plettner, E., Belzunces, L.P., Le Conte, Y., 2010. Nosema spp. infection alters pheromone production in honey bees (Apis mellifera). Chem. Ecol. 36, 522-525. https://doi.org/10.1007/s10886-010-9786-2
  12. Ellis, J.D., Graham, J.R., Mortensen, A., 2013. Standard methods for wax moth research. J. Apic. Res. 52, http://dx.doi.org/10.3896/IBRA.1.52.1.10
  13. El-Shemy, A.A.H., Ibrahim, Y.Y., El-kinani, D.D., 2012. Detection of Nosema ceranae Fries in Egypt and its seasonal fluctuations. Bull. Entomol. Soc. Egypt. 1, 25-37.
  14. Forsgren, E., Budge, G.E., Charriere, J.-D., Hornitzky, M.A.Z., 2013. Standard methods for European foulbrood research. J. Apic. Res. 52, http://dx.doi.org/10.3896/IBRA.1.52.1.12
  15. Forsgren, E., Fries, I., 2005. Acidic-Benzoic feed and Nosema disease. J. Apic. Sci. 49, 81-88.
  16. Freiberg, M., De Jong, D., Cox-Foster, D., 2013. First report of sacbrood virus in honey bee (Apis mellifera) colonies in Brazil. Genet. Mol. Res. 11, 3310-3314.
  17. Fries, I., 1988. Comb replacement and Nosema disease (Nosema apis Z.) in honey bee colonies. Apidologie 19, 343-354. https://doi.org/10.1051/apido:19880402
  18. Fries, I., Chauzat, M.-P., Chen, Y.-P., Doublet, V., Genersch, E., Gisder, S., Higes, M., McMahon, D.P., Martin-Hernandez, R., Natsopoulou, M., Paxton, R.J., Tanner, G., Webster, T.C., Williams, G.R., 2013. Standard methods for Nosema research. J. Apic. Res. 52, DOI 10.3896/IBRA.1.52.1.14
  19. Furgala, B., Boch, R., 1970. The Effect of Fumidil B, Nosemack and Humatin on Nosema apis. J. Apic. Res. 9, 79-85. https://doi.org/10.1080/00218839.1970.11100251
  20. Gajger, I.T., Kozaric, Z., Berta, D., Nejedli, S., Petrinec, Z., 2011. Effect of the herbal preparation Nozevit on the mid-gut structure of honeybees (Apis mellifera) infected with Nosema sp. spores. Vet. Med. 56, 344-351. https://doi.org/10.17221/1587-VETMED
  21. Gajger, I.T., Nejedli, S., Kozaric, Z., 2013. The effect of Nozevit on leucine aminopeptidase and esterase activity in the midgut of honey bees (Apis mellifera). Vet. Med. 58, 422-429. https://doi.org/10.17221/6982-VETMED
  22. Gajger, I.T., Vugrek, O., Pinter, L., Petrinec, Z., 2009. "Nozevit patties" treatment of honey bees (Apis mellifera) for the control of Nosema ceranae disease. Am. Bee J. 149, 1053-1056.
  23. Garcia-Palencia, P., Martin-Hernandez, R., Gonzalez-Porto, A.-V., Marin, P., Meana, A., Higes, M., 2010. Natural infection by Nosema ceranae causes similar lesions as in experimentally infected cagedworker honey bees (Apis mellifera). J. Apic. Res. 49, 278-283. https://doi.org/10.3896/IBRA.1.49.3.08
  24. Giersch, T., Berg, T., Galea, F., Hornitzky, M., 2009. Nosema ceranae infects honey bees (Apis mellifera) and contaminates honey in Australia. Apidologie 40, 117-123. https://doi.org/10.1051/apido/2008065
  25. Haddad, N.J., 2014. First detection of Nosema ceranae in Jordan. Eur. Sci. J. 10, 1857-1881.
  26. Higes, M., Martin, R., Meana, A., 2006. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol. 92, 93-5. https://doi.org/10.1016/j.jip.2006.02.005
  27. Higes, M., Nozal, M.J., Alvaro, A., Barrios, L., Meana, A., Martin-hernandez, R., Bernal, J.L., Bernal, J., 2011. The stability and effectiveness of fumagillin in controlling Nosema ceranae (Microsporidia) infection in honey bees (Apis mellifera) under laboratory and field conditions. Apidologie 42, 364-377. https://doi.org/10.1007/s13592-011-0003-2
  28. Kralj, J., Fuchs, S., 2010. Nosema sp. influences flight behavior of infected honey bee (Apis mellifera) foragers. Apidologie 41, 21-28. https://doi.org/10.1051/apido/2009046
  29. Liu, T.P., 1973. Effects of Fumidil B on the spore of Nosema apis and on lipids of the host cell as revealed by freeze-etching. J. Invertebr. Pathol. 22, 364-368. https://doi.org/10.1016/0022-2011(73)90165-1
  30. Malone, L.A., Gatehouse, H.S., Tregidga, E.L., 2001. Effects of time, temperature, and honey on Nosema apis (Microsporidia: Nosematidae), a parasite of the honeybee, Apis mellifera (Hymenoptera: Apidae). J. Invertebr. Pathol. 77, 258-268. https://doi.org/10.1006/jipa.2001.5028
  31. Michalczyk, M., Sokol, R., Koziatek, S., 2016. Evaluation of the effectiveness of selected treatments of Nosema spp. infection by the hemocytometric method and duplex PCR. Acta Vet.-Beograd. 66, 115-124. https://doi.org/10.1515/acve-2016-0009
  32. Mladan, V., Todorovic, D., Lolln, M., 2000. Therapeutic effects of fumagillin and first appearance of Nosema apis spores following infection. Acta Vet.-Beograd. 50, 253-262.
  33. Nanetti, A., Rodriguez-Garcia, C., Meana, A., Martin-Hernandez, R., Higes, M., 2015. Effect of oxalic acid on Nosema ceranae infection. Res. Vet. Sci. 102, 167-172. https://doi.org/10.1016/j.rvsc.2015.08.003
  34. Paxton, R.J., Klee, J., Korpela, S., Fries, I., 2007. Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 38, 558-565. https://doi.org/10.1051/apido:2007037
  35. Porrini, M.P., Fernandez, N.J., Garrido, P.M., Gende, L.B., Medici, S.K., Eguaras, M.J., 2011. In vivo evaluation of antiparasitic activity of plant extracts on Nosema ceranae (Microsporidia). Apidologie 42, 700-707. https://doi.org/10.1007/s13592-011-0076-y
  36. Retschnig, G., Williams, G.R., Schneeberger, A., Neumann, P., 2017. Cold ambient temperature promotes Nosema spp. intensity in honey bees (Apis mellifera). Insects 8, doi:10.3390/insects8010020
  37. Spivak, M., Reuter, G.S., 2001. Resistance to American foulbrood disease by honey bee colonies Apis mellifera bred for hygienic behavior. Apidologie 32, 555-565. https://doi.org/10.1051/apido:2001103
  38. Traver, B.E., Williams, M.R., Fella, R.D., 2012. Comparison of within hive sampling and seasonal activity of Nosema ceranae in honey bee colonies. J. Invertebr. Pathol. 109, 187-193. https://doi.org/10.1016/j.jip.2011.11.001
  39. Wang, D.-I., Moeller, F.E., 1969. Histological comparisons of the development of hypopharyngeal glands in healthy and Nosemainfected worker honey bees. J. Invertebr. Pathol. 14, 135-142. https://doi.org/10.1016/0022-2011(69)90098-6
  40. Wang, D.-I., Moeller, F.E., 1971. Ultrastructural changes in the hypopharyngeal glands of worker honey bees infected by Nosema apis. J. Invertebr. Pathol. 17, 308-320. https://doi.org/10.1016/0022-2011(71)90002-4
  41. Yoshiyama, M., Kimura, K., 2011. Distribution of Nosema ceranae in the European honeybee, Apis mellifera in Japan. J. Invertebr. Pathol. 106, 263-267. https://doi.org/10.1016/j.jip.2010.10.010