Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant Effect of Rubi Fructus on TM4 Sertoli Cells

남성생식세포 Sertoli cell에 미치는 복분자(覆盆子)의 항산화 효과

  • Kim, Young Joo (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Chang, Mun Seog (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Park, Seong Kyu (Department of Prescriptionology, College of Korean Medicine, Graduate School, Kyung Hee University)
  • 김영주 (경희대학교 대학원 한의과대학 처방제형학교실) ;
  • 장문석 (경희대학교 대학원 한의과대학 처방제형학교실) ;
  • 박성규 (경희대학교 대학원 한의과대학 처방제형학교실)
  • Received : 2018.04.16
  • Accepted : 2018.05.29
  • Published : 2018.05.31
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Abstract

Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured. Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, $100{\mu}g/ml$. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of $100{\mu}g/ml$. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed. Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.

Keywords

References

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