Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate

  • Kim, Jiyong (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Kim, Hwan D. (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Park, Jungha (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Lee, Eun-seo (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Kim, Eugene (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Lee, Seunghun S. (Interdisciplinary Program in Bioengineering, Seoul National University) ;
  • Yang, Jin-Kyung (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Lee, Yoon-Sik (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University) ;
  • Hwang, Nathaniel S. (School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University)
  • Received : 2017.11.23
  • Accepted : 2017.12.12
  • Published : 2018.03.01
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Abstract

Background: Tissue engineering is an interdisciplinary field that attempts to restore or regenerate tissues and organs through biomimetic fabrication of scaffolds with specific functionality. In recent years, graphene oxide (GO) is considered as promising biomaterial due to its nontoxicity, high dispersity, and hydrophilic interaction, and these characteristics are key to stimulating the interactions between substrates and cells. Method: In this study, GO substrates were fabricated via chemically immobilizing GO at 1.0 mg/ml on glass slides. Furthermore, we examined the osteogenic responses of murine mesenchymal-like stem cells, C3H10T1/2 cells, on GO substrates. Results: C3H10T1/2 cells on GO substrates resulted in increased cell surface area, enhanced cellular adhesions, and instigated osteogenic differentiation. Furthermore, priming of C3H10T1/2 cells with chondrocyte-conditioned medium (CM) could further induce a synergistic effect of osteogenesis on GO substrates. Conclusions: All of these data suggest that GO substrate along with CM is suitable for upregulating osteogenic responses of mesenchymal stem cells.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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