Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Temperature Dependent of Mitotic Interval for Grass Puffer, Takifugu niphobles

  • Ko, Min Gyun (Dept. of Fisheries Biology Center for Risk Assessment of Oceans and Fisheries Living Modified Organisms, Pukyong National University) ;
  • Lee, Hyo Bin (Dept. of Fisheries Biology Center for Risk Assessment of Oceans and Fisheries Living Modified Organisms, Pukyong National University) ;
  • Gil, Hyun Woo (Bio-Monitoring Center) ;
  • Kang, Shin Beom (Dept. of Ocean Physical Education, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Kim, Dong Soo (Dept. of Marine Bio-Materials & Aquaculture, Pukyong National University)
  • Received : 2018.01.11
  • Accepted : 2018.03.25
  • Published : 2018.03.31
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Abstract

The objective of this study was to determine the mitotic intervals (${\tau}_0$) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and $24^{\circ}C$). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was $0.09{\pm}0.002mg$. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at $20^{\circ}C$ (p<0.05). The size of the fertilized egg of $18^{\circ}C$ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differences were observed in the other water temperatures group except $18^{\circ}C$ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and $24^{\circ}C$ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between ${\tau}_0$ and water temperature for grass puffer (Y=-1.225X+70.05, $R^2=0.988$, n=10, where Y was ${\tau}_0$ and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be helpful for this species using cleavage frequency and ${\tau}_0$.

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References

  1. Ahn CM (1995) Effects of photoperiod and water temperature on the reproductive cycle of the spring-spawning bitterling, Rhodeus uyekii (Pisces: Cyprinidae). Korean J Ichthyol 7:33-42.
  2. Dettlaff TA (1986) The rate of development in poikilothermic animals calculated in astronomical and relative time units. J Therm Biol 11:1-7. https://doi.org/10.1016/0306-4565(86)90010-0
  3. Gil HW, Lee TH, Choi CY, Kang CB, Park I-S (2017a) Anesthetic and physiological effects of clove oil and lidocaine-HCl on the grass puffer, Takifugu niphobles. Ocean Polar Res 39:1-11. https://doi.org/10.4217/OPR.2017.39.1.001
  4. Gil HW, Lee TH, Choi CY, Kang CB, Park I-S (2017b) Induction of artificial spawning by human chorionic gonadotropin in the grass puffer, Takifugu niphobles. J Fish Mar Sci Edu 29:409-414.
  5. Goo IB, Park I-S, Gil HW, Im JH (2015) Stimulation of spermiation by human chorionic gonadotropin and carp pituitary extract in grass puffer, Takifugu niphobles. Dev Reprod 19:253-258. https://doi.org/10.12717/DR.2015.19.4.253
  6. Han KH, Cho JK, Lee SH, Hwang DS, Yoo DJ (2001) Spawaning behavior and early life history of Takifugu pardalis (Teleostei: Tetraodontidae) in Korea. Kor J Ichthyol 3:181-189.
  7. Han KN (1999) Development of eggs, larvae and juveniles of the puffer, Takifugu rubripes reared in the laboratory. Japan J Ichthyol 33:186-194.
  8. Honma Y, Ozawa T, Chiba A (1980) Maturation and spawning behavior of the puffer, Fugu niphobles, occurring on the coast of Sado Island in the Sea of Japan (a preliminary report). Jpn J Ichthyol 27:129-138.
  9. Ignat'eva GM (1975) Temperature dependence of cleavage rates in carp, pike, and whitefish. Sov J Dev Biol 5:24-28.
  10. Jang SI, Kang HW, Han HG (1996) Embryonic, larval and juvenile stages in yellow puffer, Takifugu obscurus. J Aquacult 1:11-18.
  11. Kim B-S, Lim SG, Gil HW, Park I-S (2011) Temperature dependent index of mitotic interval (${\tau}_{0}$) for chromosome manipulation in Korean rose bitterling, Rhodeus uyekii. Fish Aquat Sci 14:429-433.
  12. Kim DS, Gil HW, Lee TH, Park I-S (2017) Comparative analysis of sexual dimorphism between spawning season and non-spawning season in the grass puffer, Takifugu niphobles. J Fish Mar Sci Edu 29:447-452.
  13. Masaomi H, Yutaka T, Kadoo M (2013) Gonadal development and fertility of triploid grass puffer, Takifugu niphobles induced by cold shock treatment. Mar Biotechnol 15:133-144. https://doi.org/10.1007/s10126-012-9470-3
  14. Mims SD, Shelton WL, Linhart O, Wang C (1997) Induced meiotic gynogenesis of paddlefish, Polyodon spathula. J World Aquacult Soc 28:334-343. https://doi.org/10.1111/j.1749-7345.1997.tb00280.x
  15. Nozaki M, Tsutsumi T, Kobayashi H, Takei Y, Ichikawa T, Tsuneki K, Miyagawa K, Uemura H, Tatsumi Y (1976) Spawning habit of the puffer, Fugu niphobles (Jordanet et Snyder). Zool Mag 85:156-168.
  16. Oh SH, Han KH, Kim YM, Joung HH, Shin SS, Kim YU (2000) Spawning behavior and early life history of grass puffer, Takifugu niphobles. Kor J Ichthyol 4:236-243.
  17. Park I-S, Im S-Y (2010) Egg development and mitotic interval (${\tau}_{0}$) in black plaice, Pleuronectes obscurus (Herzenstein). Fish Aquat Sci 13:278-283.
  18. Park I-S (2017) Effect of starvation on the just hatched grass puffer, Takifugu niphobles larvae. JMLS 2:27-33.
  19. Park I-S, Im JH (2001) Determination of the temperature dependent index of mitotic interval (${\tau}_{0}$) for chromosome manipulation in far eastern catfish, Silurus asotus. Korean J Ichthyol 13:85-88.
  20. Saat T, Veersalu A (1996) Duration of synchronous cleavage cycles and rate of development at different temperatures in the Baltic herring. J Fish Biol 48:658-663. https://doi.org/10.1111/j.1095-8649.1996.tb01461.x
  21. Shelton WL (1993) Determination of the developmental duration (${\tau}_{0}$) for ploidy manipulation in carps. Isr J Aquacult 45:73-81.
  22. Shelton WL, Mims SD, Clark JA, Hiott AE, Wang C (1997) A temperature-dependent index of mitotic interval (${\tau}_{0}$) for chromosome manipulation in paddlefish and shovelnose sturgeon. Prog Fish-Cult 59:229-234. https://doi.org/10.1577/1548-8640(1997)059<0229:ATDIOM>2.3.CO;2
  23. Thorgaard GH (1983) Chromosome set manipulation and sex control in fish. In: Fish Physiology. Vol. 9B. Hoar WS, Randall DJ and Donaldson EM, eds. Academic Press, New York, NY, pp 405-434.
  24. Thorgaard GH, Allen SK Jr (1987) Chromosome manipulation and markers in fishery management. In: Population Genetics and Fishery Management. Ryman N and Utter F, eds. University of Washington Press, Seattle, WA, US, pp 319-331.
  25. Thorgaard GH, Jazwin ME, Stier AR (1981) Polyploidy induced by heat shock in rainbow trout. Trans Amer Fish Soc 110:546-550. https://doi.org/10.1577/1548-8659(1981)110<546:PIBHSI>2.0.CO;2
  26. Yoo GY, Lee TH, Gil HW, Lim SG, Park IS (2018) Cytogenetic analysis of hybrid and hybrid triploid between river puffer, Takifugu obscures and tiger puffer, T. rubripes. Aquacult Res 49:637-650. https://doi.org/10.1111/are.13493