Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Preparation of Interface-Assembled Carbonyl Reductase and Its Application in the Synthesis of S-Licarbazepine in Toluene/Tris-HCl Buffer Biphasic System

  • Ou, Zhimin (Pharmaceuticals College, Zhejiang University of Technology) ;
  • Xu, Jiahui (Pharmaceuticals College, Zhejiang University of Technology) ;
  • Du, Lihua (Pharmaceuticals College, Zhejiang University of Technology) ;
  • Tang, Lan (Pharmaceuticals College, Zhejiang University of Technology) ;
  • Niu, Yangping (Linan People's Hospital) ;
  • Cui, Jian (Linan People's Hospital)
  • Received : 2017.11.20
  • Accepted : 2018.02.06
  • Published : 2018.04.28
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Abstract

In this study, interface-assembled carbonyl reductase (IACR) was prepared and used in the synthesis of S-licarbazepine in a toluene/Tris-HCl biphasic system. The carbonyl reductase (CR) was conjugated with polystyrene to form a surfactant-like structure at the interface of the toluene/Tris-HCl biphasic system. The interface-assembled efficiency of IACR reached 83% when the CR (180 U/mg) and polystyrene concentration were $8{\times}10^2g/ml$ and $3.75{\times}10^3g/ml$, respectively. The conversion reached 95.6% and the enantiometric excess of S-licarbazepine was 98.6% when $3.97{\times}10^6nmol/l$ oxcarbazepine was converted by IACR using 6% ethanol as a co-substrate in toluene/Tris-HCl (12.5:10) at $30^{\circ}C$ and $43{\times}g$ for 6 h. IACR could be reused efficiently five times.

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References

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