The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Gamidohongsamul-tang (GDT) on the Gene Expression Levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC Cells

가미도홍사물탕(加味桃紅四物湯)이 HUVEC cell 내에 eNOS, KLF2, ICAM-1, VCAM-1의 유전자 발현양에 대해 미치는 영향

  • Lim, Hyun-chan (Dept. of Oriental Internal Medicine, Dong-Shin University Gwangju Oriental Hospital) ;
  • Jeon, Sang-yoon (Dept. of Oriental Internal Medicine, Dong-Shin University Gwangju Oriental Hospital)
  • 임현찬 (동신대학교광주한방병원 한방내과) ;
  • 전상윤 (동신대학교광주한방병원 한방내과)
  • Received : 2018.02.02
  • Accepted : 2018.03.28
  • Published : 2018.03.30
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Abstract

Objectives: This study seeks to investigate the effects of Gamidohongsamul-tang (GDT) on the Gene expression levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC cells. Methods: HUVEC cells were treated at a concentration of 1, 10, 100 (${\mu}g/ml$) of Gamidohongsamul-tang (GDT). To measure the NOS, KLF2, ICAM-1 and VCAM-1 gene expression in HUVEC cells, the synthesized cDNA was subjected to polymerase chain reaction (PCR) and electrophoresis was performed to verify gene expression level. Results: 1. GDT significantly increased eNOS and KLF2 gene expression. 2. GDT significantly reduced ICAM-1 and VCAM-1 gene expression. Conclusions: These experiments suggest that Gamidohongsamul-tang (GDT) regulates gene expression related with anti-dyslipidemic effects in HUVEC cells. In order to clinically apply this to diseases related to dyslipidemia, such as cardiovascular disease, additional in vivo experiments are needed to verify the anti-dyslipidemic effects of GDT.

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