Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo

  • Nho, Jong-Hyun (National Development Institute of Korean Medicine) ;
  • Jung, Ho-Kyung (National Development Institute of Korean Medicine) ;
  • Lee, Mu-Jin (National Development Institute of Korean Medicine) ;
  • Jang, Ji-Hun (National Development Institute of Korean Medicine) ;
  • Sim, Mi-Ok (National Development Institute of Korean Medicine) ;
  • Jeong, Da-Eun (National Development Institute of Korean Medicine) ;
  • Cho, Hyun-Woo (National Development Institute of Korean Medicine) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University)
  • Received : 2017.11.08
  • Accepted : 2018.03.13
  • Published : 2018.04.15
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Abstract

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.

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References

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