Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Optimal hot water extraction conditions of fermented Polygonum multiflorum root by Lentinula edodes pegler mycelials using response surface methodology

반응표면 분석법에 의한 표고균사체발효 적하수오 열수 추출조건의 최적화

  • Oh, Junseok (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Hong, Jae-Heoi (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Park, Tae-Young (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Shin, Ji-eun (Department of Information Statistics, Chungnam National Universitiy) ;
  • Kim, Kyung-Je (Jangheung Research Institute for Mushroom Industry) ;
  • Jin, Seong-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Ban, Seung-Eon (Jangheung Research Institute for Mushroom Industry) ;
  • Koh, Young-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Im, Seung-Bin (Jangheung Research Institute for Mushroom Industry) ;
  • Seo, Kyoung-Sun (Jangheung Research Institute for Mushroom Industry)
  • 오준석 (동부생약영농조합법인) ;
  • 홍재희 (동부생약영농조합법인) ;
  • 박태영 (동부생약영농조합법인) ;
  • 신지은 (충남대학교 정보통계학과) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 진성우 ((재)장흥군버섯산업연구원) ;
  • 반승언 ((재)장흥군버섯산업연구원) ;
  • 고영우 ((재)장흥군버섯산업연구원) ;
  • 임승빈 ((재)장흥군버섯산업연구원) ;
  • 서경순 ((재)장흥군버섯산업연구원)
  • Received : 2017.12.08
  • Accepted : 2018.03.08
  • Published : 2018.03.31
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Abstract

This study was performed to determine optimal extraction conditions of fermented Polygonum multiflorum root by Lentinula edodes (JMI10079) Pegler mycelials using response surface methodology. The independent factors were extraction temperature (X1: $40-100^{\circ}C$), extraction time (X2: 2-10 hrs.), and the ratio of water to sample (X3: 33-100 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents (Y1), $^{\circ}Brix$ of sample extract (Y2), total polyphenol content (Y3), total flavonoid content (Y4), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation scavenging activity (Y5) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (Y6). The experimental data obtained were fit to a second-order polynomial equation. The optimal extraction conditions for fermented P. multiflorum root were: X1: $91.22^{\circ}C$, X2: 7.72 hrs, and X3: 39.71 mg/mL.

본 연구는 표고균사체 발효 적하수오 열수 최적 추출조건 탐색을 목적으로 수행하였으며, 표고균사로 배양된 하수오의 독립변수로는 독립변수(Xi)로서 추출온도(X1), 추출시간(X2) 및 시료에 대한 용매비(X3)에 대한 실험범위를 설정하여 각각을 5단계로 부호화하였고 중심합성계획에 따라 16구로 설정하여 추출하였다. 또한 이들 독립변수에 영향을 받을 종속변수(Yn)로는 고형분 함량(Y1) 및 당도(Y2), TPC (total polyphenol contents, Y3), TFC (total flavonoid contents, Y4), ABTS cation radical scavenging activity (Y5), DPPH radical scavenging activity (Y6)으로 하였다. 고용분 함량 최대값과 최소값은 각각 31.99%와 16.84%로 나타났다. 이를 회귀분석한 결과 고형분 함량에 대한 모델식의 $R^2$은 0.867로 나타났다. 당도는 7번(8 hrs, $85^{\circ}C$, 67 mg/mL)과 15번(6 hrs, $70^{\circ}C$, 100 mg/mL)시험구에서 3.0 brix로 다른 시험구에 비하여 높은 함량을 보였다. 항산화물질인 총 폴리페놀과 플라보노이드 함량은 8번 시험구(8 hrs, $85^{\circ}C$, 40 mg/mL)에서 총 폴리페놀 함량이 30.40 mg GAE/g, 총 플라보노이드 함량이 146.50 mg QE/g으로 가장 높게 나타났으며, 16가지 시험구의 항산화 활성도 총 폴리페놀과 총 플라보노이드 함량의 결과와 같이 8번 시험구(8 hrs, $85^{\circ}C$, 40 mg/mL)에서 가장 높은 활성을 보였다. 이상의 결과를 참조하여 표고균 사체 발효 적하수오에 대한 열수 추출조건을 최적화할 목적으로 추출온도를 $95^{\circ}C$로 고정하고 용매비와 추출시간에 대한 종속변수들의 contour map을 superimposing하여 추출물의 특성 중 생리활성물질 함량과 생리활성을 만족시켜주는 최적조건은 $91.22^{\circ}C$에서 39.71 mg/mL 용매비와 7.72 추출시간으로 각각 분석되었다. 본 연구는 적하수오에 표고균사체를 배양한 후 생리활성이 우수한 원료를 추출 생산하고자 최적 추출조건을 확립하기 위하여 반응표면분석법을 활용하였다. 향후 생리활성부분에 대한 추가적인 연구가 이루어진다면 더욱 완성도가 높은 표고균사체 발효 적하수오 식품개발에 가능할 것으로 사료된다.

Keywords

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