Fig. 1. Germination rate of Aspergillus nidulans spores in the presence of sugar. Asexual spores of A. nidulans were incubated in the solution of water (○), 5% of glucose (●), D-arabinose (△), and L-arabinose (▲) at 37℃, respectively. Germination rate was calculated by counting the spores formed germ tubes.
Fig. 2. Germination rate of Aspergillus nidulans spores in the presence of mixture of D- and Larabinose. Spores were incubated in the solution containing various molar ratio of D- and L- arabinose. Germination rate was calculated by counting the germinated spores by time interval.
Fig. 3. Germination rate of Aspergillus nidulans spores treated with glucose and L-arabinose for short period. Asexual spores were treated with 5% of glucose and L-arabinose for 5 min, respectively. After washing out 3 times with water, spores were incubated in water without sugar for 6 hr. The germ tubes were observed by inverted microscopy after incubation (ⅹ400).
Fig. 4. Germination rate of Aspergillus nidulans spores treated with trypsin. Spores were incubated with trypsin for 2 hr. After washing out added trypsin, spores were further incubated in the presence of 5% glucose at 37℃ (□). The spores treated with BSA instead of trypsin were used as control (■).
Fig. 5. Analysis of proteins extracted from spore surface of Aspergillus nidulans by SDS-polyacrylamide gel electrophoresis. Proteins extracted from asexual spores using 1 M NaCl were treated with/without trypsin. The arrows and numbers indicated protein band to be cut out for protein identification by MALDI-TOF mass spectrometry.
Table 1. The germination rate of Aspergillus nidulans spores incubated in the presence of various sugars
Table 2. List of identifed proteins extracted from spore surface of Aspergillus nidulans
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