한국균학회지 (The Korean Journal of Mycology)

  • 제46권4호
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  • Pages.495-503
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  • 2018
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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원목 및 톱밥배지 버섯 재배사 내 실내 공기서 분리한 미기록 진균 보고

Unrecorded Fungal Species Isolated from Indoor Air in the Log Bed- and Sawdust Media-based Mushroom Cultivation Houses

  • 투고 : 2018.11.12
  • 심사 : 2018.11.19
  • 발행 : 2018.12.01
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초록

2016년 4월부터 6월까지 표고 원목재배 임가 4곳과 표고 톱밥재배 임가 1곳의 재배사 내 실내 공기에서 진균을 포집 및 분리 동정하였다. 총 6개의 국내 미기록 진균(Cenangium acuum, Periconia macrospinosa, Neopestalotiopsissurinamensis, Metarhiziummarquandii, Trichoderma petersenii, Trichoderma paratroviride)을 분리하였으며 이들 균류에 대하여 형태학적 특성 및 internal transcribed spacer rDNA region, large subunitregion, ${\beta}-tubulin$ 또는 translation elongation factor $1{\alpha}$유전자 염기서열에 기반하여 계통학적 분석 결과를 기술하였다.

Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and ${\beta}-tubulin$ gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

키워드

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Fig. 1. Example of indoor air uptake using air samplers in two different oak mushroom cultivation houses. A, log bed-based mushroom cultivation houses; B, sawdust media-based mushroom cultivation houses.

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Fig. 2. Unrecorded fungal species isolated from mushroom cultivation houses in this study. The fungi were grown on potato dextrose agar at 25℃ for 7 days. A, Cenangium acuum; B, Periconia macrospinosa; C, Neopestalotiopsis surinamensis; D, Metarhizium marquandii; E, Trichoderma petersenii; F, Trichoderma paratroviride.

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Fig. 3. Microscopic image of unrecorded fungal species isolated from mushroom cultivation houses in this study. A, Cenangium acuum; B, Periconia macrospinosa; C, Neopestalotiopsis surinamensis; D, Metarhizium marquandii; E, Trichoderma petersenii; F, Trichoderma paratroviride. Red arrow is important feature (scale bar = 2 μm).

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Fig. 4-2. Phylogenic relationships of the six fungal isolates inferred by the neighbor joining analysis based on 28S rDNA (A), internal transcribed spacer rDNA (B, D), β-tubulin gene (C) or the translation elongation factor 1a gene (E, F) sequences. D, Metarhizium marquandii (NIBRFGC000499882); E, Trichoderma petersenii (NIBRFGC000499883); F, Trichoderma paratroviride (NIBRFGC000499884).

GNHHDL_2018_v46n4_495_f0005.png 이미지

Fig. 4-2. Phylogenic relationships of the six fungal isolates inferred by the neighbor joining analysisbased on 28S rDNA (A), internal transcribed spacer rDNA (B, D), β-tubulin gene (C) or the translationelongation factor 1a gene (E, F) sequences. D, Metarhizium marquandii (NIBRFGC000499882); E,Trichoderma petersenii (NIBRFGC000499883); F, Trichoderma paratroviride (NIBRFGC000499884).

Table 1. List of the fungal species isolated and identifed in this study from mushroom cultivation houses in this study

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