Fig. 1. Culture and morphological characteristics of Leptosphaerulina australis KNU16-004. A, Colony on oatmeal agar; B, Colony on potato dextrose agar; C, Colony on malt extract agar; D, Colony on synthetic low-nutrient a gar; E, G, Colony sporulating on ascomata; F, Ascospores; H, Asci (scale bar = 10 μm).
Fig. 2. Neighbor-joining phylogenetic tree based on a concatenated alignment of the large subunit, internal transcribed spacer, and β-tubulin sequences. The phylogenic analysis shows the position of Leptosphaerulina australis KNU16-004 among related Leptosphaerulina spp. strains. Bootstrap values (based on 1,000 replications) greater than 50% are shown at the branch points. Filled circles indicate that the corresponding nodes were also recovered in trees generated with the maximum-likelihood and maximum-parsimony algorithms. Open circles indicate that the corresponding nodes were also recovered in the tree generated with the maximum-likelihood algorithm. The tree was rooted using Herpotrichia juniperi CBS 468.64 as an outgroup. Bar, 0.02 substitutions per nucleotide position. CBS, Westerdijk Fungal Biodiversity Institute (formerly CBSKNAW), Utrecht, The Netherlands.
Table 1. Isolates used in this study and their GenBank accession numbers
Table 2. Morphological characteristics of KNU16-004 isolated in this study and comparison with previously reported isolates
참고문헌
- McAlpine D. Fungus diseases of stone fruits in Australia and their treatment. Melbourne: R. S. Brain, Government Printer; 1902.
- Graham JH, Luttrell ES. Species of Leptosphaerulina on forage plants. Phytopathology 1961;51:680-93.
- Irwin JA, Davis RD. Taxonomy of some Leptosphaerulina spp. on legumes in eastern Australia. Aust J Bot 1985;33:233-7. https://doi.org/10.1071/BT9850233
- Crivelli PG. Uber die heterogene Ascomycetengattung Pleospora rabh.: Vorschlag fur eine Aufteilung [dissertation]. Zurich: ETH Zurich; 1983.
- Abler SW. Ecology and taxonomy of Leptosphaerulina spp. associated with turfgrasses in the United States [dissertation]. Blacksburg (VA): Virginia Polytechnic Institute and State University; 2003.
- Zhang Y, Crous PW, Schoch CL, Hyde KD. Pleosporales. Fungal Divers 2012;53:1-221. https://doi.org/10.1007/s13225-011-0117-x
- Couch HB. Diseases of turfgrasses. Malabar: Krieger Publishing Co.; 1995.
- Wehmeyer LE. The development of the ascocarp in Pseudoplea gaeumannii. Mycologia 1955;47:163-76. https://doi.org/10.1080/00275514.1955.12024441
- Park S, Ten L, Lee SY, Back CG, Lee JJ, Lee HB, Jung HY. New recorded species in three genera of the Sordariomycetes in Korea. Mycobiology 2017;45:64-72. https://doi.org/10.5941/MYCO.2017.45.2.64
- Raja HA, Miller AN, Pearce CJ, Oberlies NH. Fungal identification using molecular tools: a primer for the natural products research community. J Nat Prod 2017;80:756-70. https://doi.org/10.1021/acs.jnatprod.6b01085
- Rehner SA, Samuels GJ. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res 1994;98:625-34. https://doi.org/10.1016/S0953-7562(09)80409-7
- Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- Woudenberg JH, Aveskamp MM, de Gruyter J, Spiers AG, Crous PW. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 2009;22:56-62. https://doi.org/10.3767/003158509X427808
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_ X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
- Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser (Oxf) 1999;41:95-8.
- Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20. https://doi.org/10.1007/BF01731581
- Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
- Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368-76. https://doi.org/10.1007/BF01734359
- Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406-16. https://doi.org/10.2307/2412116
- Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
- Hyun IH, Heo NY, Chang SY, Heo JY, Mel'nik V. Identification of three fungi newly intercepted from importing plants in Korea. Mycobiology 2005;33:243-4. https://doi.org/10.4489/MYCO.2005.33.4.243
- Crous PW, Summerell BA, Swart L, Denman S, Taylor JE, Bezuidenhout CM, Palm ME, Marincowitz S, Groenewald JZ. Fungal pathogens of Proteaceae. Persoonia 2011;27:20-45. https://doi.org/10.3767/003158511X606239
- Mitkowski NA, Browning M. Leptosphaerulina australis associated with intensively managed stands of Poa annua and Agrostis palustris. Can J Plant Pathol 2004;26:193-8. https://doi.org/10.1080/07060660409507131