Journal of Plant Biotechnology

  • 제45권4호
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  • Pages.357-363
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  • 2018
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Induced freezing tolerance and free amino acids perturbation of spinach by exogenous proline

  • Shin, Hyunsuk (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Oh, Sewon (Department of Horticulture, Chungbuk National University) ;
  • Kim, Daeil (Department of Horticulture, Chungbuk National University) ;
  • Hong, Jeum Kyu (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Yun, Jae Gil (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Lee, Sang Woo (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Son, Ki-Ho (Department of Horticulture, Gyeongnam National University of Science and Technology)
  • 투고 : 2018.12.17
  • 심사 : 2018.12.17
  • 발행 : 2018.12.31
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초록

The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.

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참고문헌

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