Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Phylogeny of Yeasts Isolated from the Flower of Aster spathulifolius Maxim. and Screening of Biosurfactant Producers

해국(Aster spathulifolius Maxim.)의 꽃에서 분리한 효모의 분자계통학적 분포해석 및 생물계면활성제 생산 균주의 스크리닝

  • Kim, Jong-Shik (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Kim, Dae-Shin (World Heritage Office, Jeju Special Self-Governing Provincial Government)
  • 김종식 (경북해양바이오산업연구원) ;
  • 김대신 (세계유산.한라산연구원)
  • Received : 2018.09.13
  • Accepted : 2018.10.16
  • Published : 2018.12.31
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Abstract

BACKGROUND: Yeast biotechnology finds applications in various industries. Hence, we sought to explore the yeasts associated with the flower of Aster spathulifolius Maxim. This study aimed to isolate yeasts from the flower of the plant and screen for biosurfactant-producing yeasts. METHODS AND RESULTS: We collected flowers of Aster spathulifolius Maxim. and performed pure isolation using four types of media. In total, 117 strains belonging to 4 genera, namely, Cryptococcus (75 strains), Aureobasidium pullulans (30 strains), Candida (11 strains), and Rhodotorula (1 strain), were isolated and identified by ITS sequencing. Upon in-depth analysis, Cryptococcus, the most dominant genus (75 strains) was categorized into the 'Unknown group'. Upon in-depth analysis of A. pullulans, we discovered the 'Unknown group I' (27 strains) and the 'Unknown group II' (2 strains), which have not been reported previously. Two A. pullulans isolates with potent surfactant activity were selected via the screening procedure. CONCLUSION: In this study, a total of 117 strains were isolated from the flower of Aster spathulifolius Maxim. In addition, two biosurfactant-producing yeasts were identified from among the isolated yeasts.

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References

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