Journal of Plant Biotechnology

  • 제45권1호
  • /
  • Pages.77-82
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  • 2018
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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2014년 경기지역 유전자변형 옥수수 모니터링 및 발견현황

Detection and environmental unintentional release monitoring of living modified maize (Zea mays L.) in Gyeonggi-do of South Korea in 2014

  • 신수영 (국립생태원 생태보전연구실) ;
  • 문정찬 (국립생태원 생태보전연구실) ;
  • 최원균 (국립생태원 생태보전연구실) ;
  • 김일룡 (국립생태원 생태보전연구실) ;
  • 조범호 (국립생태원 생태보전연구실) ;
  • 이중로 (국립생태원 생태보전연구실)
  • Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Moon, Jeong Chan (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Choi, Wonkyun (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Kim, Il Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Jo, Beom-Ho (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Lee, Jung Ro (Division of Ecological Conservation, National Institute of Ecology (NIE))
  • 투고 : 2017.12.04
  • 심사 : 2017.12.15
  • 발행 : 2018.03.31
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초록

국내에서 LM 작물의 재배는 승인 된 바 없으나 식품용 및 사료용으로 LM 작물이 수입되고 있으며 LM 작물의 국내 수입량은 꾸준히 증가하고 있는 추세이다. 본 연구는 2014년 경기 지역의 LM 옥수수 유출여부를 모니터링 하기 위해 수행되었으며 채집된 의심시료는 단백질 검정 분석법과 중합효소 연쇄반응을 통해 분석하였다. 경기 지역의 항만, 운송로, 사료공장 및 축산농가 등 총 169개 지점을 조사하였고 총 44개의 LM 옥수수 의심시료를 채집하였다. 단백질 검정 분석법으로 4개의 양성반응 시료를 확인하였고 동일지점의 시료는 혼합하여 총 3개의 시료에 대해 프로모터 스크리닝과 이벤트 특이적 프라이머를 이용한 중합효소 연쇄반응을 실시하여 의심시료 모두 LM 옥수수로 확인하였다. 본 연구의 결과는 국내 자연환경에서 LM 옥수수의 유출 현황 파악과 자연 생태계로 LM 옥수수의 유출을 예방하기 위해 자연생태계 모니터링이 매우 중요하다는 것을 뒷받침한다.

In South Korea, LM crops are not allowed to grow locally, but have been allowed to be imported as food and feed purposes. Currently, the typical LMO imports are continuously increasing in the region of South Korea. In 2014, we carried out a review of the environmental release monitoring of LM maize (Zea mays L.) in Gyeonggi-do of South Korea, and analyzed volunteer samples using strip test kits and polymerase chain reaction (PCR) methods. We thereby collected 44 volunteers of released LM maize in 169 locations around ports, from roadsides, feed factories and stockbreeding farmhouses. We found 4 positive samples at 3 sites using strip test kits. Based on the PCR analysis, the LM maize plants were found using event-specific primers. These results suggested that our monitoring is necessary to detect the presence of released LM maize in the natural environment of South Korea.

키워드

참고문헌

  1. European Commission, Joint Research Centre (JRC). Available from: http://gmo-crl.jrc.ec.europa.eu
  2. Han SM, Kim DY, Uddin MR, Hwang KS, Lee BK, Kim CG and Park KW (2014) Appearance/Instance of genetically modified maize at grain receiving harbors and along transportation routes in Korea. Weed Turf Sci. 3:221-224 https://doi.org/10.5660/WTS.2014.3.3.221
  3. International Service for the Acquisition of Agri-biotech Applications (ISAAA). GM Approval Database. Available from: http://www.isaaa.org. Accessed 2015
  4. Kim CG, Yi HB, Park SK, Yeon JE, Kim DY, Kim DI, Lee KH, Lee TC, Paek IS, Yoon WK, Jeong SC and Kim HM (2006) Monitoring the occurrence of genetically modified soybean and maize around cultivated fields and at a grain receiving port in Korea. J. Plant Biol. 49:218-223 https://doi.org/10.1007/BF03030536
  5. KBCH: Korea Biosafety Clearing House. [Internet]. 2015. Status of risk assessment of GMO in Korea. Available from: http://www.Biosafety.or.kr
  6. Kim YK (2015) Development status and prospects of GM crops. Korean J. Int. Agric. 27(4):448-454 https://doi.org/10.12719/KSIA.2015.27.4.448
  7. Lee BK, Kim CG, Park JY, Park KW, Kim HJ, Yi HB, Jeong SC, Yoon WK and Kim HM (2009) Monitoring the occurrence of genetically modified soybean and maize in cultivated fields and along the transportation routes of the Incheon port in South Korea. Food Control 20:250-254 https://doi.org/10.1016/j.foodcont.2008.05.006
  8. Mazzara M., Puumalaainen J., Van Den Eede G. (2005) Validation of the GMO specific detection method developed by NVI/INRA for Bt11 in sweet corn Maize - validation report and protocol
  9. Mazzara M., Paoletti C., Puumalaainen J., Rasulo D., Van Den Eede G. (2005) Event-specific method for the quantitation of Maize line NK603 using real-time PCR - validation report and protocol
  10. Mazzara M., Grazioli E., Savini C., Van Den Eede G. (2009) Report on the verification of the performance of a MON810 event-specific method on Maize line MON810 using real-time PCR - validation report and protocol
  11. National Institute of Ecology (NIE) (2014) Study on environmental monitoring and post-management of LMO (VI). NIE-2014-0039
  12. National Institute of Ecology(NIE) (2015) Detection methods of living modified organisms. ISBN 979-11-86197-24-0
  13. Han SM, Lee JJ, Won OJ, Eom MY and Park KW (2015) Occurrence and distribution of genetically modified Maize (Zea mays L.) at a grain receiving port and along transportation routes in Korea. Korean J. Int. Agric. 27(3):321-325 https://doi.org/10.12719/KSIA.2015.27.3.321
  14. Savini C., Bogni A., Grazioli E., Munaro B., Mazzara M., Van Den Eede G. (2008) Event-specific method for the quantification of Maize line MON89034 using real-time PCR - validation report and protocol
  15. Shin SY, Jo BH, Moon JC, Jung Ro Lee, Choi WK, Seol MA, Kim MJ, Song HR (2016) Detection of LM canola with tolerance to glyphosate and glufosinate ammonium via the environmental monitoring in South Korea. J Plant Biotechnol 43:479-485 https://doi.org/10.5010/JPB.2016.43.4.479