The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Neuroprotective Effects of Schisandra chinensis and Ribes fasciculatum Extract on Hydrogen Peroxide-Mediated Oxidative Stress in Neuroblastic SH-SY5Y Cell Line

과산화수소로 유도된 SH-SY5Y 신경세포 사멸에 대한 오미자·칠해목 추출혼합물의 보호효과

  • 박은국 (아주대학교 의과대학 의학유전학) ;
  • 한경훈 (서울의료원 의학연구소) ;
  • 이승희 (서울의료원 의학연구소) ;
  • 김남기 ((주)알피바이오) ;
  • 배문형 ((주)알피바이오) ;
  • 서영하 ((주)알피바이오) ;
  • 용윤중 ((주)나인비) ;
  • 정선용 (아주대학교 의과대학 의학유전학) ;
  • 최춘환 ((재)경기도경제과학진흥원 바이오센터)
  • Received : 2018.10.26
  • Accepted : 2018.12.04
  • Published : 2018.12.31
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Abstract

In neuronal cell deaths, oxidative stress is normally implicated with a most of these deaths occurring in neurodegenerative disorders such as the Alzheimer's and Parkinson's diseases. In this study, the neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts on hydrogen peroxide ($H_2O_2$)-induced oxidative stress in neuroblastic cell line were investigated. For an hour, hydrogen peroxide of $100{\mu}M$ concentration, was induced on neuroblastic cells, causing apoptic cell death. For the neuroprotection, a sample of neuroblastic cells had been pre-treated with SC and RF extracts for 24 hours before application of the hydrogen peroxide. No neurotoxic effects were observed in the cells that had been treated by SC and RF. This prove that the treatment of SC and RF extract prevented apoptotic cell death of neuroblastic cell line exposed to oxidative injury. In addition, applying both SC and RF extracts at a 7:3 ratio increased the neuronal cell survival rate, compared to individual treatments of SC and RF extract. This study suggests that SC and RF extracts may be potential therapeutic agents for the prevention of neuronal cell death.

Keywords

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Fig. 1. Cell survival rate of H2O2 concentrations in SHSY5Y cells.

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Fig. 2. The effects of different ethanol extracts on cell survival rate.

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Fig. 3. The effects of Schisandra chinensis and Ribes fasciculatum on cell survival rate and proliferation.

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Fig. 4. Comparison of cell survival rate of single or combined Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in SH-SY5Y cells.

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Fig. 5. Effects of different ratio of combined Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in neuronal cells.

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Fig. 6. Inhibitory effects of combined Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts on apoptotic neuronal cell death.

References

  1. Baral S, Pariyar R, Yoon CS, Yun JM, Jang SO, Kim SY, Oh H, Kim YC, Seo J. 2015. Effects of Schisandra fructus 70% ethanol extract on proliferation and differentiation of human embryonic neural stem cells. Korean J Pharmacogn 46:52-58
  2. Bidchol AM, Wilfred A, Abhijna P, Harish R. 2011. Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. FoodBioprocess Technol 4: 1137-1143 https://doi.org/10.1007/s11947-009-0196-9
  3. Byun MW, Byun EH. 2015. Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts. J Korean Soc Food Sci Nutr 44:182-190 https://doi.org/10.3746/jkfn.2015.44.2.182
  4. Cha JY, Yoo HR, Kim YS, Seol IC, Jo HK. 2017. Inhibition of gene associated with dyslipidemia and antioxidative effect of Artemisia iwayomogi, Curcumae Radix and Raphani Semen (ACR) on HepG2 cell model. J Korean Med 38:43-58 https://doi.org/10.13048/jkm.17024
  5. Choi JR, Choi JM, Lee S, Cho KM, Cho EJ, Kim HY. 2014. The protective effects of protocatechuic acid from Momordica charantia against oxidative stress in neuronal cells. Korean J Pharmacogn 45:11-16
  6. Comporti M. 2012. Free radicals, oxidative stress, and antioxidants. J Siena Acad Sci 2:13-26 https://doi.org/10.4081/jsas.2010.13
  7. Giridharan VV, Thandavarayan RA, Sato S, Ko KM, Konishi T. 2011. Prevention of scopolamine-induced memory deficits by schisandrin B, an antioxidant lignan from Schisandra chinensis in mice. Free Radic Res 45:950-958 https://doi.org/10.3109/10715762.2011.571682
  8. Han KH, Kim DH, Song KY, Lee SW, Han SH. 2015. The effects of methanol extract from cheonggukjang in T98G cells and early stage of focal ischemia rodent models. Korean J Food Nutr 28:965-972 https://doi.org/10.9799/ksfan.2015.28.6.965
  9. Han KH, Lee SH, Park KS, Song KY, Kim JH, Park EK, Han SH. 2017. Protective effect of PineXol(R) against amyloid-${\beta}$-induced cell death. Korean J Food Nutr 30:1279-1285
  10. Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY. 2004. Potent inhibitory effect of flavonoids in Scutellaria baicalensis on amyloid ${\beta}$ protein-induced neurotoxicity. J Agric Food Chem 52:4128-4132 https://doi.org/10.1021/jf049953x
  11. Jeong CH, Kwak JH, Kim JH, Choi GN, Jeong HR, Heo HJ. 2010. Neuronal cell protective effects of methanol extract from Cheonggukjang using in vitro system. Korean J Food Sci Technol 42:768-772
  12. Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY. 2014. Ribes fasciculatum var. Chinense attenuated allergic inflammation in vivo and in vitro. Biomol Ther 22:547-552 https://doi.org/10.4062/biomolther.2014.015
  13. Kim Hk, Na GM, Ye SH, Han HS. 2004. Extraction characteristics and antioxidative activity of Schiznadra chinensis extracts. J Korean Soc Food Cult 19:484-490
  14. Kim HS, Moon HK, Lee YJ, Lee CY, Hwang KH, Kim OH, Yoo IS, Jung K. 2015. Comparison of the content of shizandrin, gomisin A and gomisin N in schisandra fruit by water extraction condition. J Food Hyg Saf 30:59-64 https://doi.org/10.13103/JFHS.2015.30.1.59
  15. Kim JH, Jeong CH, Choi GN, Kwak JH, Choi SG, Heo HJ. 2009. Antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis using an in vitro system. Korean J Food Sci Technol 41:712-716
  16. Kim K, Hahn B, Choe W. 2012. $H_2O_2$ induced two type of cell death in human hepatoma cell, Huh7. Cancer Prev Res 17:15-18
  17. Kim SY, Kim MJ, Ahn KJ, An IS, An SK. 2015. Antioxidant effects of kinetin on HaCaT human keratinocytes damaged by hydrogen peroxide ($H_2O_2$). Korean J Aesthet Cosmetol 13:59-64
  18. Lee KS, Lee EK, Seo YH, Choe SY. 2016 Effects of the mixture of fenugreek seeds and Lespedeza cuneata extracts on testosterone synthesis in TM3 cells oxidative stressed with $H_2O_2$. J Appl Biol Chem 59:305-311
  19. Lee MS, Chao J, Yen JC, Lin LW, Tsai FS, Hsieh MT, Peng WH, Cheng HY. 2012. Schizandrin protects primary rat cortical cell cultures from glutamate-induced apoptosis by inhibiting activation of the MAPK family and the mitochondria dependent pathway. Molecules 18:354-372 https://doi.org/10.3390/molecules18010354
  20. Lenaz G, 1998. Role of mitochondria in oxidative stress and aging. Biochim Biophys Acta 1366:53-67 https://doi.org/10.1016/S0005-2728(98)00120-0
  21. Oh DH, Koh SH, Chung B, Park KH, Kim HY, Song CW, Kim YC, Kim JH, Kim MH, Kim SH. 2006. Cytoprotective effect of 15-deoxy-delta (12,14) prostaglandin J2 (15d-PGJ2) against $H_2O_2$ induced death of neuronally-differentiated PC12 cells. J Korean Neurol Assoc 24:58-65
  22. Papa S, Skulachev VP. 1997. Reactive oxygen species, mitochondria, apoptosis and aging. pp.305-319. Springer
  23. Park SH, Park SK, Ha JS, Lee DS, Kang JY, Kim JM, Lee UK, Heo HJ. 2016. Effect of gomchwi (Ligularia fischeri) extract against high glucose- and $H_2O_2$-induced oxidative stress in PC12 cells. Korean J Food Sci Technol 48:508-514 https://doi.org/10.9721/KJFST.2016.48.5.508
  24. Piao XL, Kim HY, Yokozawa T, Lee YA, Piao XS, Cho EJ. 2005. Protective effects of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage. J Nutr Sci Vitaminol 51:142-147 https://doi.org/10.3177/jnsv.51.142
  25. Wang CP, Li GC, Shi YW, Zhang XC, Li JL, Wang ZW, Ding F, Liang XM. 2014. Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons. J Physiol Biochem 70:735-747 https://doi.org/10.1007/s13105-014-0342-3
  26. Woo C, You JY, Jang CY, Kim HR, Shin YJ, Moon AJ, Shin SH. 2014. Protective effects of sosokmyoung-tang against Parkinson’s model in human neuroblastoma SH-SY5Y cells. J Int Korean Med 35:298-308
  27. Xu X, Zhou X, Zhou XW, Zhang Z, Liao MJ, Gao Q, Luo HM. 2012. Schizandrin prevents dexamethasone-induced cognitive deficits. Neurosci Bull 28:532-540 https://doi.org/10.1007/s12264-012-1258-y
  28. Zeng KW, Zhang T, Fu H, Liu GX, Wang XM. 2012. Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the toll-like receptor 4-dependent MyD88/IKK/NF-${\kappa}B$ signaling pathway in lipopolysaccharide-induced microglia. Eur J Pharmacol 692:29-37 https://doi.org/10.1016/j.ejphar.2012.05.030

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