Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of MMP-2 activation and FSH or LH Hormone Supplementation on Embryo Development in In Vitro Fertilization of Porcine

  • Kim, Sang Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Yoon, Jong Taek (Institute of Genetic Engineering, Hankyong National University)
  • Received : 2018.12.18
  • Accepted : 2018.12.20
  • Published : 2018.12.31
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Abstract

The purpose of this study was to analyze whether FSH and LH hormone treatment directly or indirectly affect embryo development in embryonic development. To determine this, we compared the development of embryonic cells through the expression pattern of MMPs. As a result, 33.8% of blastocysts were formed in FSH added group, 20.8% in LH added group and 10% in FSH + LH added group. In addition, the activity of MMP-9 was highly detected in the FSH-added group, and the expression of Casp-3 was much lower than that of the other groups. These results suggest that the addition of FSH seems to increase the activity of MMP-9 in embryonic cells, and that LH, on the contrary, may activate MMP-2 activity. In addition, the expression level of MMP-2 in the FSH-added group was high in the Trophoblast cell group and in the LH-added group, the hormone ideal secretion might affect the development of the embryonic cell.

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References

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