Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지
DOI QR코드

DOI QR Code

Complete genome sequence of Lactobacillus koreensis 26-25, a ginsenoside converting bacterium, isolated from Korean kimchi

김치에서 분리한 진세노사이드 전환 능력이 있는 Lactobacillus koreensis 26-25의 유전체 서열 분석

  • Kim, Ju-Hyeon (Department of Biotechnology, Hankyong National University) ;
  • Liu, Qing-Mei (Department of Biotechnology, Hankyong National University) ;
  • Srinivasan, Sathiyaraj (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Sang Yong (Department of Food Science & Bio Technology, Shinansan University) ;
  • Wee, Ji-Hyang (Department of Food Science & Bio Technology, Shinansan University) ;
  • Im, Wan-Taek (Department of Biotechnology, Hankyong National University)
  • 김주현 (국립한경대학교 농업생명과학대학 생명공학과) ;
  • 류청매 (국립한경대학교 농업생명과학대학 생명공학과) ;
  • 스리니바산 사티야라지 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 김명겸 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 김상용 (신안산대학교 식품생명과학과) ;
  • 위지향 (신안산대학교 식품생명과학과) ;
  • 임완택 (국립한경대학교 농업생명과학대학 생명공학과)
  • Received : 2018.11.08
  • Accepted : 2018.12.13
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A Gram-positive, rod-shaped, ivory colored, and motile, Lactobacillus koreensis 26-25 was isolated from Korean kimchi. Strain 26-25 showed the ability of conversion from major ginsenosides into minor ginsenosides for which whole genome was sequenced. The whole genome sequence of Lactobacillus koreensis 26-25 consisted of one circular chromosome comprised of 3,006,812 bp, with a DNA G + C content of 49.23%. The whole genome analysis of strain 26-25 showed many glycosides hydrolase genes, which may contribute to identify the genes responsible for transformation of major ginsenosides into minor ginsenosides for its high pharmacological effects.

김치로부터 분리한 Lactobacillus koreensis 26-25 균주의 유전체서열을 분석하였다. 균주 26-25의 유전체는 G + C 비율이 49.23%이며, 2,720개의 유전자와 2,556개의 단백질 코딩 유전자, 85개의 위유전자 그리고 78개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으면 그 크기는 3,006,812 bp였다. 균주 26-25는 인삼사포닌의 당 분해에 관여하는 여러 타입의 글라이코시다제 유전자를 가지고 있었다. 이러한 지놈 분석은 주요 진세노사이드가 우수한 약리학적 활성의 미량 진세노사이드로 전환하는데 관여하는 유전자 특징을 이해하는데 큰 기여가 되었다.

Keywords

MSMHBQ_2018_v54n4_477_f0001.png 이미지

Fig. 1. Graphical map of the genome of Lactobacillus koreensis 26-25.

Table 1. General features of Lactobacillus koreensis 26-25

MSMHBQ_2018_v54n4_477_t0001.png 이미지

References

  1. Antunes A, Rainey FA, Nobre MF, Schumann P, Ferreira AM, Ramos A, Santos H, and da Costa MS. 2002. Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov. Int. J. Syst. Evol. Microbiol. 52, 647-655. https://doi.org/10.1099/00207713-52-2-647
  2. Beijerinck MW. 1901. Sur les ferments lactiques de l'industrie. Arch. Ne'er. Sci. Exactes Natur. (Section 2) 6, 212-243.
  3. Kandler O and Weiss N. 1986. Regular nonsporing Gram-positive rods, pp. 1208-1234. In Sneath PH, Mair N, Sharpe ME, and Holt JG. (eds.), Bergey's manual of systematic bacteriology, vol 2, William and Wilkins, Baltimore, MD, USA.
  4. Siddiqi MZ, Muhammad Shafi S, and Im WT. 2017. Complete genome sequencing of Arachidicoccus ginsenosidimutans sp. nov., and its application for production of minor ginsenosides by finding a novel ginsenoside-transforming ${\beta}$-glucosidase. RSC Adv. 7, 46745. https://doi.org/10.1039/C7RA02612A