생약학회지 (Korean Journal of Pharmacognosy)

  • 제49권1호
  • /
  • Pages.40-46
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  • 2018
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

모과 부탄올 분획의 예쁜꼬마선충 내의 항산화 효과

Antioxidant Activity of n-Butanol Fraction of Chaenomeles sinensis Fruit in Caenorhabditis elegans

  • 투고 : 2018.03.09
  • 심사 : 2018.03.20
  • 발행 : 2018.03.31
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초록

Chaenomeles sinensis (Thouin) Koehne fruit (Rosaceae) has been used as a traditional medicine in Korea, Japan and China to treat sore throat, diarrhea and inflammation. The ethanol extract of C. sinensis fruit was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions, the n-butanol fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities, the n-butanol fraction was checked the activities of superoxide dismutase (SOD) and catalase activities, and intracellular ROS levels and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of the n-butanol fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, the n-butanol fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, the n-butanol fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

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