Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Stress Responses of Olive Flounder (Paralichthys olivaceus) to Salinity Changes

염분변화에 따른 넙치(Paralichthys olivaceus)의 스트레스 반응

  • Park, Hyung-Jun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Min, Byung-Hwa (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
  • 박형준 (국립수산과학원 양식관리과) ;
  • 민병화 (국립수산과학원 동해수산연구소 양식산업과)
  • Received : 2018.01.17
  • Accepted : 2018.03.13
  • Published : 2018.03.31
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Abstract

We tried to determine the optimum salinity for a cultured of olive flounder (Paralichthys olivaceus) by investigating after exposing the fish at different salinity (10, 15, 20 and 25 psu) for 24 and 48 hours compared with control group (fish before transfer to experimental tank). As a control groups, we compared an analyzed with other experimental groups using olive flounder in natural sea water. Hematological parameters including hematocrit (Ht) and hemoglobin (Hb), cortisol and glucose, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), $NH_3$, osmolality, total protein (TP), $Na^+$, $K^+$ and $Cl^-$ mostly exhibited significant changes at 10 and 15 psu groups compared with control groups for 24 and 48 hours exposed. Plasma SOD (superoxide dismutase) and CAT (catalase) activity also increased with experimental groups (10 and 15 psu) compared to the control groups. The expression of HSP70 mRNA was also higher at low-salinity (10 and 15 psu) than at control group. In particular, after 24 hours exposed, it expression to 15 psu groups showed a significant difference compared to the control group. However, after 48 hours exposed, it expression was higher in the 10 psu groups than the control. It is assumed that the changes in the hematological responses and hormone, homeostasis and metabolism were resulted in to protect fish body from stress. Based on these results, we are expected that it will be used as basic data for the culture of olive flounder prepared for low salinity.

스트레스를 유발하는 환경적인 요소 중, 염분의 변화는 어류의 성장과 생존에 있어 많은 영향을 미친다. 본 연구에서는 한국의 양식대상 어종인 넙치(P. olivaceus)를 대상으로 넙치양식에 있어 적정 염분범위를 구명하기 위하여 각 염분별(25, 20, 15 psu 및 10 psu) 조건을 통해 24시간 및 48시간 동안 노출시킨 후, 혈액생리학적 분석 및 HSP70 mRNA 발현을 조사하였다. 혈액학적 분석에서 hematocrit (Ht) 및 hemoglobin (Hb), 혈장 코티졸 (cortisol) 및 글루코스 (glucose)의 변화, aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT), $Na^+$, $K^+$, $Cl^-$, $NH_3$, 삼투질농도(osmolality) 및 총 단백질(total protein, TP)는 저염분 조건인 10 psu 및 15 psu에서 다른 염분별 실험구에 비해 대부분의 항목에서 유의적인 차이를 보였다. 혈장 내 SOD (superoxide dismutase) 및 CAT(catalase) 활성에서도 마찬가지로 대조구인 자연해수의 조건보다 저염분(10 psu 및 15 psu)으로 갈수록 증가하는 경향을 보였다. 스트레스성 단백질로 알려진 HSP 70 mRNA 발현에서도 대조구보다 저염분에서 유의적인 차이를 확인하였다. 이러한 결과로, 염분변화에 의한 스트레스가 넙치의 생체방어메커니즘과 항상성 유지를 위한 결과로 혈액학적 반응, 호르몬의 분비 및 단백질 발현의 증가가 나타나는 것으로 여겨지며, 이를 바탕으로 저염분에 대비한 넙치양식에 있어 기초적인 자료로 활용될 것이 기대된다.

Keywords

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