한국축산식품학회지 (Food Science of Animal Resources)

  • 제38권6호
  • /
  • Pages.1226-1236
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  • 2018
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

  • Lee, Jae Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Ahn, Dong Uk (Department of Animal Science, Iowa State University) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • 투고 : 2018.10.16
  • 심사 : 2018.11.14
  • 발행 : 2018.12.31
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초록

Ovotransferrin (OTF) is a well-known protein of the transferrin family with strong iron chelating activity, resulting in its antimicrobial activity. Furthermore, OTF is known to have antioxidant, anticancer, and antihypertensive activities. However, there have been few studies about the immune-enhancing activity of OTF. In current study, we investigated the immune-enhancing activity of OTF using the murine macrophage cells in vitro. The effect of OTF on production of pro-inflammatory mediators and cytokines were determined using Griess assay and quantitative real-time PCR. Using Neutral Red uptake assay, we confirmed the effect of OTF on phagocytic activity of macrophages. Ovotransferrin significantly increased the production of nitric oxide (NO) and secretion of inducible nitric oxide synthase (iNOS) mRNA with no cytotoxic activity. Ovotransferrin (2 mg/mL) stimulated NO production up to $31.9{\pm}3.5{\mu}M$. Ovotransferrin significantly increased the mRNA expression levels of pro-inflammatory cytokines which are tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), Interleukin-$1{\beta}$ (IL-$1{\beta}$), and IL-6: OTF (2 mg/mL) treatment increased the secretion of mRNA for TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 by 22.20-, 37.91-, and 6.17-fold of the negative control, respectively. The phagocytic activity of macrophages was also increased by OTF treatment significantly compared with negative control. Also, OTF treatment increased phosphorylation level of MAPK signaling pathways. These results indicated that OTF has immune-enhancing activity by activating RAW 264.7 macrophages via MAPK pathways.

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과제정보

연구 과제 주관 기관 : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)

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  2. The potential of lactoferrin, ovotransferrin and lysozyme as antiviral and immune-modulating agents in COVID-19 vol.15, pp.9, 2020, https://doi.org/10.2217/fvl-2020-0170
  3. Phosphoproteomic analysis of duck egg white and insight into the biological functions of identified phosphoproteins vol.44, pp.10, 2020, https://doi.org/10.1111/jfbc.13367
  4. Iridin Prevented Against Lipopolysaccharide-Induced Inflammatory Responses of Macrophages via Inactivation of PKM2-Mediated Glycolytic Pathways vol.14, pp.None, 2018, https://doi.org/10.2147/jir.s292244
  5. Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review vol.30, pp.5, 2018, https://doi.org/10.1007/s10068-021-00901-3
  6. Enzymatic Hydrolysis of Ovotransferrin and the Functional Properties of Its Hydrolysates vol.41, pp.4, 2018, https://doi.org/10.5851/kosfa.2021.e19
  7. The past and future of ovotransferrin: Physicochemical properties, assembly and applications vol.116, pp.None, 2021, https://doi.org/10.1016/j.tifs.2021.07.003
  8. Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells vol.9, pp.11, 2021, https://doi.org/10.1002/fsn3.2569