Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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The Antioxidative Effect of Eclipta prostrata L. Extract on Cultured NIH3T3 Fibroblasts Injured by Manganese-Induced Cytotoxicity

  • Lee, Sang-Hee (Sanbon Hospital, School of Medicine, Wonkwang University) ;
  • Jung, In-Ju (Department of Cosmetology, Dongshin University) ;
  • Jang, Hyesook (Department of Nursing, Jeonju University)
  • Received : 2018.11.07
  • Accepted : 2018.12.13
  • Published : 2018.12.31
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Abstract

Manganese (Mn) is used as main materials in various chemical processes of industry, but it suggested that Mn brings about its toxicant by fume or dust through respiratory system and skin barrier. Mn toxicant induces the loss of mental health and life quality by cerebrovascular and skin diseases. Nevertheless, it lefts much unknown on the mechanism and the effectively therapeutic methods about Mn toxicant. Therefore, this study was evaluated the cytotoxicity induced by manganese dioxide ($MnO_2$) in cultured NIH3T3 fibroblasts, and also, the correlation between $MnO_2$-induced cytotoxicity and oxidative stress was examined. While, the effect of Eclipta prostrata L. (EP) extract belong to Compositae was assessed against $MnO_2$-induced cytotoxicity in the view of antioxidative effect for searching the natural resources mitigating or preventing the $MnO_2$-induced cytotoxicity. In this study, $MnO_2$-induced cytotoxicity was revealed as mid-toxic by Borenfreud and Puerner's toxic criteria, and catalase (CAT), an antioxidant prevented $MnO_2$-induced cytotoxicity by the remarkable increase of cell viability in these cultures. While, in the protective effect of EP extract on $MnO_2$-induced cytotoxicity, EP extract effectively prevented the cytotoxicity induced by $MnO_2$ via antioxidative effects such as xanthine oxidase (XO) inhibitory ability and DPPH-radical scavenging ability. From the above results, EP extract showed the effective prevention against $MnO_2$-induced cytotoxicity correlated with oxidative stress by antioxidative effects. Conclusively, this study may be useful to research or development the alternatively therapeutic agent from natural resources like EP extract for the treatment of diseases resulted in oxidative stress.

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References

  1. A.O.A.C. Official method of analysis. 18th ed., Association of official analytical chemists. Washington DC USA. 2005. 45: 21-21.
  2. Arditi T, Meredith T, Floweman P. Renewed interest in soy isoflavones and saponin. Cereal Foods World. 2000. 45: 414-417.
  3. Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 1958. 26: 1199-1200.
  4. Borenfreund E, Puerner JA. A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90). Journal of Tissue Culture Methods. 1984. 9: 7-9.
  5. Chang JK. Wild flower for body health. 1st ed. Seoul: Nexusbook academic publishing; 2003. p.150.
  6. Cho KS. Occupational health. 2nd ed. Seoul: Sumunsa academic publishing; 1991. pp. 315-319.
  7. Fulle S, Withers-Martinez C, Blackman MJ, Morris GM, Finn PW. Molecular determinants of binding to the plasmodium subtilism -like protease 1. Journal of Chemistry and Inflammatory Model. 2013. 53: 573-583. https://doi.org/10.1021/ci300581z
  8. Hwang JD, Choi JS, Kim JB, Lee YS. Antioxidant activities of bark extracts from Kalopanax pictus. Journal of Investigative Cosmetology. 2011. 7: 329-337. https://doi.org/10.15810/jic.2011.7.4.001
  9. Jin EY, Han SH, Seo YM. Effect of German chemomile extract on the cell adhesion activity and melanin synthesis in cultured human skin melanoma cells damaged by xanthine oxidase/hypoxanthine. Journal of Korean Society People Plants Environment. 2011. 14: 215-220.
  10. Jung JY, Oh SK, Park SH, Yoon MY, Yu YW, Rim YS, Jung IJ. Antioxidative effect of Ajuga multiflora BUNG extract on chromium trioxide, dermatitis induced in cultured NIH3T3 fibroblast. Journal of Investigative Cosmetology. 2014a. 10: 21-26. https://doi.org/10.15810/jic.2014.10.1.003
  11. Jung JY, Oh YH, Park SH, Yoon MY, Pyo AJ, et al. Antioxidative and whitening effects of Houttuynia cordata extract on lead acetate of hair dye component. Journal of Investigative Cosmetology. 2014b. 10: 99-105.
  12. Jung JY, Jang HS, Seo YM. Protective effect of Ajuga multiflora BUNG extract on lead toxicity of environmental pollutant. Journal of Korean Society People Plants Environment. 2017a. 20: 341-350. https://doi.org/10.11628/ksppe.2017.20.4.341
  13. Jung IJ, Seo YM, Jekal SJ. The protective effect of rosmarinic acid on the aluminum of dementia inducer. Korean Journal of Clinical Laboratory Society. 2017b. 49: 8-14. https://doi.org/10.15324/kjcls.2017.49.1.8
  14. Kim JC, So BK. Traditional drugs of the East. 1st ed. Seoul: Youngrim academic publishing; 1995. p.339.
  15. Kim TY, Jekal SJ. Antioxidative effect of Chelidonium majus extract on cultured NIH3T3 fibroblasts injured by cadmium chloride of toxicant. Korean Journal of Clinical Laboratory Society. 2016. 48: 1-7. https://doi.org/10.15324/kjcls.2016.48.1.1
  16. Lim JA, Oh HJ, Baek SH. Antiaging ability of methanol extract from Euonymus alatus. Journal of Cosmetology and Public Health. 2007. 3: 41-45.
  17. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application of proliferation and cytotoxic assays. Journal of Immunological Methods. 1983. 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  18. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the three radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethmopharmacology. 2000. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  19. Pearce N, Checkoway H, Dement JM. Design and conduct of occupational epidermology studies: III. Design aspects of casecontrol studies. American Journal of Industrial Medicine. 1989. 15: 395-401. https://doi.org/10.1002/ajim.4700150404
  20. Son YW, Rim YS, Seo YM. Protective effect of NMDA receptor antagonist on the neurotoxicity induced by lead as an environmental pollutant. Journal of Korean Society of Occupational and Environmental Hygiene. 2017. 27: 193-200.
  21. Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase. Journal of Biology and Chemistry. 1969. 244: 3855-3861.