Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지
DOI QR코드

DOI QR Code

Cytotoxic Evaluation of Plant Essential Oils in Human Skin and Lung Cells

  • Ahn, Changhwan (Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Mi-Jin (Division of wood chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Kim, Jae-Woo (Division of wood chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Yang, Jiyoon (Division of wood chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Lee, Sung-Suk (Division of wood chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Jeung, Eui-Bae (Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University)
  • Received : 2018.01.09
  • Accepted : 2018.03.12
  • Published : 2018.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

Plant essential oils are defined as fragrant volatile oils extracted from leaves, stems, fruits, flowers, and roots of a plant. Such oils are composed of multiple components and multiple functions. By accumulation of inductive information, various plant essential oils have been studied for using in therapeutic medicine for various diseases. Despite of the apparent advantages of essential oils as a source of therapeutic medicines, plant essential oils have many limitations, including cytotoxic side effects. Therefore, it is necessary to evaluate the toxicity and the mechanisms of cytotoxicity of such oils. In this study, we evaluated the cytotoxicity to human-derived cell lines of 10 plant essential oils provided by National Institute of Forest Science (i.e., Larix kaempferi; Abies holophylla; Zanthoxylum ailanthoides; Pinus parviflora; Tsuga sieboldti; Chamaecyparis pisifera; Cryptomeria japonica; Pinus densiflora; Illicium anisatum; Pinus thunbergii). Cytotoxicity evaluations were accomplished by using CCK-assays and PCR-based cytotoxicity-related marker gene analyses with A549 cell line, and the Detroit551 cell line which are lung and skin cell line. The genes were analyzed included caspase-3 has a role in cell apoptosis, and the other cyclinA, cyclinB, cyclinD, and cyclinE regulated cell cycling for the cell proliferation. By examining the five cytotoxicity-related marker genes by performing real-time PCR and examined the cytostatic gene regulation associated with the various essential oils. The results of this study showed that the degree of cytotoxicity and the cytostatic gene regulation which could give precious information for using the plant essential oil for the clinical usages.

Keywords

References

  1. Bravo, R., Celis, J.E. 1980. A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells. The Journal of Cell Biology 84(3): 795-802. https://doi.org/10.1083/jcb.84.3.795
  2. Citron, B.A., Arnold, P.M., Sebastian, C., Qin, F., Malladi, S., Ameenuddin, S., Landis, M.E., Festoff, B.W. 2000. Rapid upregulation of caspase-3 in rat spinal cord after injury: mRNA, protein, and cellular localization correlates with apoptotic cell death. Experimental Neurology 166(2): 213-226. https://doi.org/10.1006/exnr.2000.7523
  3. Duronio, R.J., Xiong, Y. 2013. Signaling pathways that control cell proliferation. Cold Spring Harbor Perspectives in Biology 5(3): a008904. https://doi.org/10.1101/cshperspect.a008904
  4. Elledge, S.J. 1996. Cell cycle checkpoints: preventing an identity crisis. Science 274(5293): 1664-1672. https://doi.org/10.1126/science.274.5293.1664
  5. Galindo, L.A., Pultrini Ade, M., Costa, M. 2010. Biological effects of Ocimum gratissimum L. are due to synergic action among multiple compounds present in essential oil. Jounal of Nature Medicine 64(4): 436-441.
  6. Goldwasser, F., Shimizu, T., Jackman, J., Hoki, Y., O'Connor, P.M., Kohn, K.W., Pommier, Y. 1996. Correlations between S and G2 arrest and the cytotoxicity of camptothecin in human colon carcinoma cells. Cancer Research 56(19): 4430-4437.
  7. Grana, X., Reddy, E.P. 1995. Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs). Oncogene 11(2): 211-219.
  8. Ha, M.W., Ma, R., Shun, L.P., Gong, Y.H., Yuan, Y. 2005. Effects of allitridi on cell cycle arrest of human gastric cancer cells. World Journal of gastroenderology 11(35): 5433-5437. https://doi.org/10.3748/wjg.v11.i35.5433
  9. Jeong, J.H., An, J.Y., Kwon, Y.T., Rhee, J.G., Lee, Y.J. 2009. Effects of low dose quercetin: cancer cell-specific inhibition of cell cycle progression. Journal of Cellular Biochemistry 106(1): 73-82. https://doi.org/10.1002/jcb.21977
  10. Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S., Heinonen, M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry 47(10): 3954-3962. https://doi.org/10.1021/jf990146l
  11. Lambert, R.J., Skandamis , P.N., Coote, P.J., Nychas, G.J. 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology 91(3): 453-462. https://doi.org/10.1046/j.1365-2672.2001.01428.x
  12. Lee, H.R., Kim, G.H., Choi, W.S., Park, I.K. 2017. Repellent Activity of Apiaceae Plant Essential Oils and their Constituents Against Adult German Cockroaches. Journal of Econimic Entomology 110(2): 552-557. https://doi.org/10.1093/jee/tow290
  13. Lee, S.Y., Kim, S.H., Park, M.J., Lee, S.S., Choi, I.G. 2014. Antibacterial Activity of Essential Oil from Abies holophylla against Respiratory Tract Bacteria. Journal of the Korean Wood Science and Technology 42(5): 533-542. https://doi.org/10.5658/WOOD.2014.42.5.533
  14. Min, H.J., Kim, C.S., Hyun, H.J., Bae, Y.S. 2017. Essential Oil Analysis of Illicium anistum L. Extracts. Journal of the Korean Wood Science and Technology 45(6): 682-688. https://doi.org/10.5658/WOOD.2017.45.6.682
  15. Muschel, R.J., Zhang, H.B., Iliakis, G., McKenna, W.G. 1991. Cyclin B expression in HeLa cells during the G2 block induced by ionizing radiation. Cancer Research 51(19): 5113-5117.
  16. Namura, S., Zhu, J., Fink, K., Endres, M., Srinivasan, A., Tomaselli, K.J., Yuan, J., Moskowitz, M.A. 1998. Activation and cleavage of caspase-3 in apoptosis induced by experimental cerebral ischemia. Journal of Neuroscience 18(10): 3659-3668. https://doi.org/10.1523/JNEUROSCI.18-10-03659.1998
  17. Ohtsubo, M., Theodoras, A.M., Schumacher, J., Roberts, J.M., Pagano, M. 1995. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition. Molecular and Cellular Biology 15(5): 2612-2624. https://doi.org/10.1128/MCB.15.5.2612
  18. Pagano, M., Pepperkok, R., Verde, F., Ansorge, W., Draetta, G. 1992. Cyclin A is required at two points in the human cell cycle. EMBO Journal 11(3): 961-971.
  19. Porter, A.G., Janicke, R.U. 1999. Emerging roles of caspase-3 in apoptosis. Cell Death & Differentiation 6(2): 99-104. https://doi.org/10.1038/sj.cdd.4400476
  20. Prabuseenivasan, S., Jayakumar, M., Ignacimuthu, S. 2006. In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine 6: 39. https://doi.org/10.1186/1472-6882-6-39
  21. Russo, R., Corasaniti, M.T., Bagetta, G., Morrone, L.A. 2015. Exploitation of cytotoxicity of some essential oils for translation in cancer therapy. Evidence-Based Complementary and Alternative Medicine 2015: 397821.
  22. Schmidt, M., Fernandez de Mattos, S., van der Horst, A., Klompmaker, R., Kops, G.J., Lam, E.W., Burgering, B.M., Medema, R.H. 2002. Cell cycle inhibition by FoxO forkhead transcription factors involves downregulation of cyclin D. Mollecualr and Cellular Biolology 22(22): 7842-7852.
  23. Sherr, C.J. 1994. G1 phase progression: cycling on cue. Cell 79(4): 551-555. https://doi.org/10.1016/0092-8674(94)90540-1
  24. Sienkiewicz, M., Glowacka, A., Kowalczyk, E., Wiktorowska-Owczarek, A., Jozwiak-Bebenista, M., Lysakowska, M. 2014. The biological activities of cinnamon, geranium and lavender essential oils. Molecules 19(12): 20929-20940. https://doi.org/10.3390/molecules191220929
  25. Wang, Y., Baskerville, S., Shenoy, A., Babiarz, J.E., Baehner, L., Blelloch, R. 2008. Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation. Nature Genetics 40(12): 1478-1483. https://doi.org/10.1038/ng.250
  26. Waseem, R., Low, K.H. 2015. Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry. Journal of Separation Science 38(3): 483-501. https://doi.org/10.1002/jssc.201400724
  27. Zhang, Q., Wu, X., Liu, Z. 2016. Primary Screening of Plant Essential Oils as Insecticides, Fumigants, and Repellents Against the Health Pest Paederus fuscipes (Coleoptera: Staphylinidae). Journal of Econimic Entomology 109(6): 2388-2396. https://doi.org/10.1093/jee/tow232