Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Discrimination of Floral Scents and Metabolites in Cut Flowers of Peony (Paeonia lactiflora Pall.) Cultivars

  • Ahn, Myung Suk (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Pue Hee (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwon, Young Nam (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Mekapogu, Manjulatha (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Suk Weon (Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jie, Eun Yee (Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Jae Ah (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Jong Taek (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwon, Oh Keun (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2018.09.07
  • Accepted : 2018.12.17
  • Published : 2018.12.31
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Abstract

Floral scents and metabolites from cut flowers of 14 peony cultivars (Paeonia lactiflora Pall.) were analyzed to discriminate different cultivars and to compare the Korean cultivar with the other cut peonies imported to Korea using electronic nose (E-nose) and Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis, respectively. Principal component analysis (PCA) and discriminant function analysis (DFA) dendrogram of peony floral scents were not precisely same but there were 3 groups including same cultivars. PCA and partial least squares-discriminant analysis (PLS-DA) dendrograms of peony metabolites showed that different cut peony cultivars were clustered into two major groups including same cultivars. Fragrance pattern of Korean 'Taebaek' was classified to same group with 'Jubilee' on the PCA and DFA results and its metabolite pattern was clearly discriminated by the PCA and PLS-DA compared to the other cultivars. These results show that the 14 peony cut flowers could be discriminated corresponding to their chemical relationship and the metabolic profile of Korean 'Taebaek' has distinctive characteristics. Furthermore, we suggest that these results could be used as the preliminary data for breeding new cut peony cultivars and for improving the availability of Korean cut peony in cosmetic industry.

Keywords

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Fig. 1. Cut flowers of 14 cultivars of P. lactiflora at full bloom stage. (A) Blush queen, (B) Bowl of cream, (C) Jubilee, (D) Peter brand, (E) Honey gold, (F) Snow mountain, (G) Ole faithful, (H) Florence Nichols, (I) Nick Shaylor, (J) Paul M. wild, (K) Allan rogers, (L) Elsa sass, (M) Sarah bernhardt, (N) Taebaek.

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Fig. 2. Floral scent analysis of different P. lactiflora cut flower cultivars using E-nose. (A) PCA, (B) DFA, (C) Radar Plot, (D) Intensity. AR, Allan rogers; BC, Bowl of cream; BQ, Blush queen; ES, Elsa sass; FN, Florence nichols; HG, Honey gold; JB, Jubilee; NS, Nick Shaylor; OF, Ole faithful; PB, Peter brand; PM, Paul M. wild; SB, Sarah bernhardt; SM, Snow mountain; TB, Taebaek.

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Fig. 3. HCA dendrogram from PCA (A) and DFA (B) of E-nose data from P. lactiflora cut flower cultivars.

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Fig. 4. Multivariate analysis of FT-IR spectra from the flower extracts of P. lactiflora cut flower cultivars. (A) Representative FT-IR spectra from P. lactiflora cultivars. Arrows represent the FT-IR spectral regions showing significant variations among P. lactiflora cultivars. (B) PCA score plot devised from the FT-IR spectra of P. lactiflora cultivars. (C) PC loading values from the FT-IR spectra of P. lactiflora cultivars. Arrows represent significant spectral regions for determining PC 1 and 2 scores. (D) PLS-DA score plot of FT-IR data from P. lactiflora cultivars. AR, Allan rogers; BC, Bowl of cream; BQ, Blush queen; ES, Elsa sass; FN, Florence nichols; HG, Honey gold; JB, Jubilee; NS, Nick Shaylor; OF, Ole faithful; PB, Peter brand; PM, Paul M. wild; SB, Sarah bernhardt; SM, Snow mountain; TB, Taebaek.

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Fig. 5. HCA dendrogram from PCA (A) and PLS-DA (B) of FT-IR spectra from the flower extracts of P. lactiflora cut flower cultivars.

Table 1. Morphological characteristics of P. lactiflora cultivars

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Table 2. Analytical conditions for E-nose operation

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