Fig. 3. Virus titer measured in the medium of PRRS virus infected tissue. The supernatants from infected tissues were collected at 2, 4, 6 dpi. The plaque titers were determined at appropriate time points on MARC-145 cells.
Fig. 1. (A) Lung tissues from ex vivo culture in physiological conditions were stained with H&E. Histopathological examinations were performed and compared between 1 hour and 6 days after cultivation. Scale bar is 50 μm. (B) Lung tissues from ex vivo culture in physiological conditions were performed with TUNEL assay and compared between the groups on 6 days after cultivation. 3,3'-Diaminobenzidine (DAB) was used for visualizing the apoptosis as brown color. Counterstaining was performed with hematoxylin. Scale bar corresponds to 50 μm. (C) Percentage of positive area with TUNEL assay on day 6 lung tissue in physiological condition. X axis represented the each group and Y axis represented the percentage of positive area. Different letters indicate significant differences according to Duncan’s multiple range test. Value of P <0.05 was considered statistically significant based on results of one-way ANOVA. (D) Results of LDH released from lung tissues with physiological conditions on each time point. The supernatants from each group were collected at 1 hour and 2, 4, 6 days post cultivation.
Fig. 2. (A) Lung tissues which were infected with PRRS virus from ex vivo culture were stained with H&E. Histopathological examinations were performed and compared between the groups on 6 dpi. Scale bar is 100 μm. (B) Lung tissues infected with PRRS virus from ex vivo culture were performed with TUNEL assay and compared between the groups on 6 dpi. 3,3'-Diaminobenzidine (DAB) was used for visualizing the apoptosis as brown color. Counterstaining was performed with hematoxylin. Scale bar corresponds to 50 μm. (C) Percentage of positive area with TUNEL assay on day 6 lung tissue infected with PRRS virus. X axis represented the each group and Y axis represented the percentage of positive area. Different letters indicate significant differences according to Duncan’s multiple range test. Value of P <0.05 was considered statistically significant based on results of one-way ANOVA. (D) Results of LDH released from lung tissues with virus-infected conditions on each time point. The supernatants from each group were collected at 1 hour and 2, 4, 6 dpi.
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