International Journal of Advanced Culture Technology

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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Effects of Korean Radish on DSS-Induced Ulcerative Colitis in Mice

  • Kim, Hyun-Kyoung (Department of Food Science and Engineering, Seowon University)
  • Received : 2018.11.04
  • Accepted : 2018.11.16
  • Published : 2018.12.31
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Abstract

The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling moleculesthrough suppression both mitogen-activated protein kinases(MAPK) and nuclear factor-kappa B ($NF-{\kappa}B$) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazine.

Keywords

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Figure 1. SRE alleviated dextran sodium sulfate-induced experimental colitis.

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Figure 2. SRE decreased serum ROS and NADPH oxidase activity in colon.

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Figure 3. Effect of SRE on anti-oxidant proteins in DSS-induced colitis mice.

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Figure 4. c-Fos, p-p38, and p-ERK protein expressions in DSS-induced colitis.

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Figure 5. p- IκBa and NF-κBp65 protein expressions in DSS-induced colitis.

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Figure 6. COX-2, iNOS, MCP-1, ICAM-1, TNF- , and IL-1 protein expressions in DSS-induced colitis.

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Figure 7. Bax, Bcl-2, and Caspase 3 protein expressions in DSS-induced colitis.

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