Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of the Antioxidant and Antiproliferative Properties of a Hot-water Extract from Gulfweed, Sargassum fulvellum

  • Kim, So Jung (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology) ;
  • Kang, Mingyeong (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology)
  • Received : 2018.11.12
  • Accepted : 2018.12.11
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Sargassum fulvellum (gulfweed) is a widespread seaweed in the coastal areas of northeast Asia. In the present study, we identified the phenolic compounds present in aqueous and ethanolic extracts of S. fulvellum and evaluated their antioxidative properties and their abilities to block cell proliferation using in vitro assays: antioxidant activity was assessed by using a DPPH assay and superoxide anion scavenging activity, anti-tyrosinase activity, and anti-proliferative activity were assessed using MTT and lactate dehydrogenase [LDH] assays in vascular smooth muscle cells. The hot-water ($65^{\circ}C$) extract had a higher phenol content than the ethanolic extract. The hot-water extract showed a statistically significant increase in free radical scavenging activity and a greater ability to reduce proliferation of vascular smooth muscle cells stimulated with platelet-derived growth factor-BB. Taken together, hot-water extracts of S. fulvellum may be an important source of antioxidative and antiproliferative agents.

Keywords

References

  1. Meenakshi S., Umayaparvathi S., Arumugam M. and Balasubramanian T. 2011. In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar. Asian Pac. J. Trop. Biomed. 1(1, Supplement), S66-S70. https://doi.org/10.1016/S2221-1691(11)60126-3
  2. Cavas L. and Yurdakoc K. 2005. An investigation on the antioxidant status of the invasive alga Caulerpa racemosa var. cylindracea (Sonder) Verlaque, Huisman, et Boudouresque (Caulerpales, Chlorophyta). J. Exp. Mar. Biol. Ecol. 325(2), 189-200. https://doi.org/10.1016/j.jembe.2005.05.002
  3. Huang H.-L. and Wang B.-G. 2004. Antioxidant Capacity and Lipophilic Content of Seaweeds Collected from the Qingdao Coastline. J. Agri. Food Chem. 52(16), 4993-4997. https://doi.org/10.1021/jf049575w
  4. Mayer A. M., Rodriguez A. D.,, Berlinck R. G. and Hamann M. T. 2009. Marine pharmacology in 2005-6: Marine compounds with anthelmintic, antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities; affecting the cardiovascular, immune and nervous systems, and other miscellaneous mechanisms of action. Biochim. Biophys. Acta. 1790(5), 283-308. https://doi.org/10.1016/j.bbagen.2009.03.011
  5. Tewari R. K., Kumar P., Sharma P. N. and Bisht S. S. 2002. Modulation of oxidative stress responsive enzymes by excess cobalt. Plant Sci. 162(3), 381-388. https://doi.org/10.1016/S0168-9452(01)00578-7
  6. Xiang, C. and D.J. Oliver, 1998. Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. The Plant Cell. 10(9), 1539-50. https://doi.org/10.1105/tpc.10.9.1539
  7. Botterweck A. A., Verhagen H., Goldbohm R. A., Kleinjans J. and van den Brandt P. A. 2000. Intake of butylated hydroxyanisole and butylated hydroxytoluene and stomach cancer risk: results from analyses in the Netherlands Cohort Study. Food Chem. Toxicol. 38(7), 599-605. https://doi.org/10.1016/S0278-6915(00)00042-9
  8. Augustyniak A., Bartosz G., Cipak A., Duburs G., Horakova L., Luczaj W., Majekova M., Odysseos A. D., Rackova L., Skrzydlewska E., Stefek M., Strosova M., Tirzitis G., Venskutonis P. R., Viskupicova J., Vraka P. S. and Zarkovic N. 2010. Natural and synthetic antioxidants: an updated overview. Free Radical Res. 44(10), 1216-62. https://doi.org/10.3109/10715762.2010.508495
  9. Wojcik M., Burzynska-Pedziwiatr I. and Wozniak L. A. 2010. A review of natural and synthetic antioxidants important for health and longevity. Curr. Med. Chem. 17(28), 3262-88. https://doi.org/10.2174/092986710792231950
  10. Kumar M., Gupta V., Kumari P., Reddy C. R. K. and Jha B. 2011. Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. J. Food Compos. Anal. 24(2), 270-278. https://doi.org/10.1016/j.jfca.2010.07.007
  11. Kumar C. S., Ganesan P., Suresh P. V. and Bhaskar N. 2008. Seaweeds as a source of nutritionally beneficial compounds-a review. J. Food Sci. Technol. 45(1), 1-13.
  12. Chan C. X., Ho C. L. and Phang S. M. 2006. Trends in seaweed research. Trends Plant Sci. 11(4), 165-6. https://doi.org/10.1016/j.tplants.2006.02.003
  13. Devi G. K., Manivannan K., Thirumaran G., Rajathi F. A. and Anantharaman P. 2011. In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pac. J. Trop. Med. 4(3), 205-11. https://doi.org/10.1016/S1995-7645(11)60070-9
  14. Rodriguez-Bernaldo de Quiros A., Lage-Yusty M. A., and Lopez-Hernandez J. 2010. Determination of phenolic compounds in macroalgae for human consumption. Food Chem. 121(2), 634-638. https://doi.org/10.1016/j.foodchem.2009.12.078
  15. Kim S. J., Woo S., Yun H., Yum S, Choi E., Do J.-R., Jo J.-H., Kim D.-G., Lee S, Lee T.-K. 2005. Total Phenolic Contents and Biological Activities of Korean Seaweed Extracts. Food Sci. Biotech. 14(6), 798-802
  16. Yuan Y. V. and Walsh N. A. 2006. Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem. Toxicol. 44(7), 1144-1150. https://doi.org/10.1016/j.fct.2006.02.002
  17. Zhu Y. Z., Huang S. H., Tan B. K., Sun J., Whiteman M. and Zhu Y. C. 2004. Antioxidants in Chinese herbal medicines: a biochemical perspective. Nat. Prod. Rep. 21(4), 478-89. https://doi.org/10.1039/b304821g
  18. Cassolato J. E., Noseda M. D., Pujol C. A., Pellizzari F. M., Damonte E. B. and Duarte M. E. 2008. Chemical structure and antiviral activity of the sulfated heterorhamnan isolated from the green seaweed Gayralia oxysperma. Carbohyd. Res. 343(18), 3085-95. https://doi.org/10.1016/j.carres.2008.09.014
  19. Kwon H.-J., Bae S.-Y., Kim K.-H., Han C.-H., Cho S.-H., Nam S.-W., Choi Y. H. and Kim B.-W. 2007. Induction of apoptosis in HeLa cells by ethanolic extract of Corallina pilulifera. Food Chem. 104(1), 196-201. https://doi.org/10.1016/j.foodchem.2006.11.031
  20. Kim A. R., Shin T. S., Lee M. S., Park J. Y., Park K. E., Yoon N. Y., Kim J. S., Choi J. S., Jang B. C., Byun D. S., Park N. K. and Kim H. R. 2009. Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J. Agric. Food Chem. 57(9), 3483-9. https://doi.org/10.1021/jf900820x
  21. Hwang S. J., Kim Y. W., Park Y., Lee H. J. and Kim K. W. 2014. Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res. 63(1), 81-90. https://doi.org/10.1007/s00011-013-0674-4
  22. Yende S. R., Harle U.N. and Chaugule B.B. 2014. Therapeutic potential and health benefits of Sargassum species. Pharmacogn. Rev. 8(15), 1-7. https://doi.org/10.4103/0973-7847.125514
  23. Capannesi C., Palchetti I., Mascini M. and Parenti A. 2000. Electrochemical sensor and biosensor for polyphenols detection in olive oils. Food Chem. 71(4), 553-562. https://doi.org/10.1016/S0308-8146(00)00211-9
  24. Wong K. H. and Cheung P. C. K. 2000, Nutritional evaluation of some subtropical red and green seaweeds: Part I - proximate composition, amino acid profiles and some physico-chemical properties. Food Chem. 71(4), 475-482. https://doi.org/10.1016/S0308-8146(00)00175-8
  25. Suh S. S., Hwang J., Park M., Park H. S. and Lee T.-K. 2014. Phenol content, antioxidant and tyrosinase inhibitory activity of mangrove plants in Micronesia. Asian Pac. J. Trop. Med. 7(7), 531-5. https://doi.org/10.1016/S1995-7645(14)60089-4
  26. Singh N. and Rajini P. S. 2004, Free radical scavenging activity of an aqueous extract of potato peel. Food Chem. 85(4), 611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
  27. Lu Y. and Foo L. Y. 2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68(1), 81-85. https://doi.org/10.1016/S0308-8146(99)00167-3
  28. Oktay M., Gulcin İ. and Kufrevioglu O.I. 2003, Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT - Food Sci. Technol. 36(2), 263-271. https://doi.org/10.1016/S0023-6438(02)00226-8
  29. Kim D. H., Kim H. J. and Chung B. W. 2006. Extraction of anti-oxidative substance from Haematococcus pluvialis using ultrasonification. J. Eng. Res. 37(0), 79-86.
  30. Calabro A. R., Konsoula R. and Barile F. A. 2008. Evaluation of in vitro cytotoxicity and paracellular permeability of intact monolayers with mouse embryonic stem cells. Toxicol. in vitro 22(5), 1273-84. https://doi.org/10.1016/j.tiv.2008.02.023
  31. Perez J., Hill B. G., Benavides G. A., Dranka B. P. and Darley-Usmar V. M. 2010. Role of cellular bioenergetics in smooth muscle cell proliferation induced by platelet-derived growth factor. Biochem. J. 428(2), 255-67. https://doi.org/10.1042/BJ20100090
  32. Raines E. W. 2004. PDGF and cardiovascular disease. Cytokine Growth F. R. 15(4), 237-54. https://doi.org/10.1016/j.cytogfr.2004.03.004
  33. Karki R., Ho O. M. and Kim D. W. 2013. Magnolol attenuates neointima formation by inducing cell cycle arrest via inhibition of ERK1/2 and NF-kappaB activation in vascular smooth muscle cells. Biochim. Biophys. Acta. 1830(3), 2619-28. https://doi.org/10.1016/j.bbagen.2012.12.015
  34. Costa L. S., Fidelis G. P., Telles C. B., Dantas-Santos N., Camara R. B., Cordeiro S. L., Costa M. S., Almeida-Lima J., Melo-Silveira R. F., Oliveira R. M., Albuquerque I. R., Andrade G. P. and Rocha H. A. 2011. Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula. Mar. Drugs. 9(6), 952-66. https://doi.org/10.3390/md9060952
  35. Tannoury M. Y., Elia J. M., Saab A. M., Makhlouf H. Y., Abboud J. S., Daou-Chabo R. J. and Diab-Assaf M. 2016. Evaluation of Cytotoxic Activity of Sargassum vulgare From the Lebanese Coast Against Jurkat Cancer Cell Line. J. App. Pharm. Sci. 6(6), 108-112.
  36. Pinteus S., Lemos M. F. L., Silva J., Alves C., Neugebauer A., Freitas R., Duarte A. and Pedrosa R. 2017. An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model. Mar. Drugs. 15(11), 353 https://doi.org/10.3390/md15110353
  37. Lau T. Y., Vittal D. F., Chew C. S. Y. and Yong W. T. L. 2014. Antiproliferative Potential of Extracts from Kappaphycus Seaweeds on HeLa Cancer Cell Lines. Sains Malays. 43(12), 1895-1900. https://doi.org/10.17576/jsm-2014-4312-11
  38. Poussier, B., Cordova A. C., Becquemin J. P. and Sumpio B. E. 2005. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. J. Vasc. Surg. 42(6), 1190-7. https://doi.org/10.1016/j.jvs.2005.08.014