Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Growth and Ginsenoside Content of One Year Old Ginseng Seedlings in Hydroponic Culture over a Range of Days after Transplanting

수경재배 시 1년생 묘삼 이식 후 경과일수에 따른 인삼의 생육 및 Ginsenoside 함량

  • 정대희 (산림청 국립산림과학원 산림약용자원연구소) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 장인배 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 유진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박기춘 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 이응호 (주식회사 이노진팜) ;
  • 김영준 (주식회사 이노진팜) ;
  • 박홍우 (산림청 국립산림과학원 산림약용자원연구소)
  • Received : 2018.10.01
  • Accepted : 2018.12.13
  • Published : 2018.12.30
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Abstract

Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution ($NO_3{^-}-N$; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; $5.31mEq/{\ell}$). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

Keywords

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Fig. 1. Change of air temperature and light intensity according to the growing days after transplanting of one-year-old ginseng seedlings in a greenhouse.

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Fig. 2 Ginsenoside content of top part and root of ginseng seedlings in hydroponics culture according to the days after transplanting.

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Fig. 3. Ginsenoside F1, F2, F3 and F4 content of leaf and stem of ginseng seedlings in hydroponics culture according to the days after transplanting.

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Fig. 4. Total ginsenoside content of ginseng seedlings in hydroponics culture according to the days after transplanting.

Table 1. Growth average of 100 ginseng seedlings used for the experiment.

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Table 2. Growth of ginseng seedlings in hydroponics culture according to the days after transplanting.

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References

  1. Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS and Yuan CS. (2002). Antidiabetic effects of Panax Ginseng berry extract and the identification of an effective component. Diabetes. 51:1851-1858. https://doi.org/10.2337/diabetes.51.6.1851
  2. Choi JE, Li X, Han YH and Lee KT. (2009). Changes of saponin contents of leaves, stems and flower-buds of Panax ginseng C. A. Meyer by harvesting days. Korean Journal of Medicinal Crop Science. 17:251-256.
  3. Cui CH, Liu QM, Kim JK, Sung BH, Kim SG, Kim SC and Im WT. (2013). Identification and characterization of Mucilaginibacter sp. stain QM49 $\beta$-glucosidase and its use in the production of the pharmaceutically active minor ginsenosides $(S)-Rh_1$ and $(S)-Rh_2$. Applied and Environmental Microbiology. 79:5788-5798. https://doi.org/10.1128/AEM.01150-13
  4. Ghaeminia M, Rajkumar R, Kor HL, Dawe GS and Tan CH. (2018). Ginsenoside Rg1 modulates medial prefrontal cortical firing and supresses the hippocampo-medial prefrontal cortical long-term potentiation. Jouranl of Ginseng Research. 42:298-303. https://doi.org/10.1016/j.jgr.2017.03.010
  5. Ha SE, Shin DH, Kim HD, Shin SM, Kim HS, Kim BH, Lee JS and Park JK. (2010). Effects of ginsenosides Rg2 on the ultraviolet B-induced DNA damage responses in HaCaT cells. Naunyu-Schmiedeberg's Archives of Pharmacology. 382:89-101. https://doi.org/10.1007/s00210-010-0522-9
  6. Han JS, Tak HS, Lee GS, Kim JS and Choi JE. (2013). Comparison of ginsenoside content according to age and diameter in Panax ginseng C. A. Meyer cultivated by direct seedling. Korean Journal of Medicinal Crop Science. 21:184-190. https://doi.org/10.7783/KJMCS.2013.21.3.184
  7. Jeong CM. (2007). Standard ginseng cultivation method. Joongbu Publiisher. Cheongju, Korea. p.530.
  8. Jimemez-Perez ZE, Singh P, Kim YJ, Mathiyalagan R, Kim DH, Lee MH and Yang DC. (2018). Applications of Panax ginseng leaves-mediated gold nanoparticles in cosmetics relation to antioxidant, moisture retention, and whitening effect on B16BL6 cells. Journal of Ginseng Research. 42:327-333. https://doi.org/10.1016/j.jgr.2017.04.003
  9. Julius BT, Leach KA, Tran TM, Mertz RA and Braun DM. (2017). Sugar transporters in plants: New insights and discoveries. Plant and Cell Physiology. 58:1442-1460. https://doi.org/10.1093/pcp/pcx090
  10. Kim GS, Hyun DY, Kim YO, Lee SE, Kwon H, Cha SW, Park CB and Kim YB. (2010). Investigation of ginsenosides in different parts of Panax ginseng cultured by hydroponics. Korean Journal of Horticultural Science and Technology. 28:216-226.
  11. Kim GS, Hyun DY, Kim YO, Lee SW, Kim YC, Lee SE, Son YD, Lee MJ, Park CB, Park HK, Cha SW and Song KS. (2008). Extraction and preprocessing methods for ginsenosides analysis of Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science. 16:446-454.
  12. Kim MK, Kang H, Baek CW, Jung YH, Woo YC, Choi GJ, Shin HY and Kim KS. (2018). Antinociceptive and anti-inflammatory effects of ginsenoside Rf in a rat model of incisional pain. Journal of Ginseng Research. 42:183-191. https://doi.org/10.1016/j.jgr.2017.02.005
  13. Korea Institute of Planing and Evaluation for Technology and Food, Agriculture, Forestry and Fisheries(IPET). (2000). Development on the method of nutrition culture for production of high quality ginseng seedling. Research Report of Korea Institute of Planing and Evaluation for Technology and Food, Agriculture, Forestry and Fisheries. p.39-72.
  14. Lee DH, Lee DS, Jung KW, Hwang GS, Lee HL, Yamabe N, Lee HJ, Eom DW, Kim KH and Kang KS. (2018a). Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells. Journal of Ginseng Research. 42:75-80. https://doi.org/10.1016/j.jgr.2016.12.013
  15. Lee HY, Choi WY, Suh YC, Song CH, Lee CG and Im HW. (2014). Cosmetic composition comprising of gingenoside F2 for skin wrinkle improvement, skin whitening or anti-acne. Korea Patent. 10,146,056,9B1.
  16. Lee JO, Kim EJ, Kim JH, Hong YH, Kim HG, Jeong D, Kim JW, Kim SH, Park CW, Seo DB, Son YJ, Han SY and Cho JY. (2018b). Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract. Journal of Ginseng Research. 42:389-399. https://doi.org/10.1016/j.jgr.2018.02.007
  17. Lee YJ, Jin YR, Lim WC, Ji SM, Choi SH, Jang SY and Lee SK. (2003). A ginsenoside-Rh1, a component of ginseng saponin, activates estrogen receptor in human breast carcinoma MCF-7 cells. Journal of Steroid Biochemistry and Molecular Biology. 84:463-468. https://doi.org/10.1016/S0960-0760(03)00067-0
  18. Lemoine RR, Camera SL, Atanassova R, Dedaldechamp F, Allario T, Pourtau N, Bonnemain JL, Laloi M, Coutos-Thevenot P, Maurousset L, Faucher M, Girousses C, Lemonnier P, Parrilla J and Durand M. (2013). Source-to-think transport of sugar and regulation by environmental factors. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2013.00272 (cited by 2018 Sep 16).
  19. Mook-Jung IH, Hong HS, Boo JH, Lee KH, Yun SH, Cheong MY, Joo IS, Hur K and Jung MW. (2001). Ginsenoside Rb1 and Rg1 improve spatial learning and increase hippocampal synaptophysin levels in mice. Journal of Neuroscience Research. 63:509-515. https://doi.org/10.1002/jnr.1045
  20. Rural Development Administration(RDA). (2013). Development of stable production technology for tomato by the improvement of greenhouse. Rural Development Administration. Suwon, Korea. p.7.
  21. Tung NH, Song GY, Kim JA, Hyun JH, Kang HK and Kim YH. (2010). Dammarane-type saponins from the flower buds of Panax ginseng and their effects on human leukemia cells. Bioorganic and Medicinal Chemistry Letters. 20:309-314. https://doi.org/10.1016/j.bmcl.2009.10.110
  22. Wang L, Zhang F, Cao Z, Xiao Y, Li S, Yu B and Qi J. (2017). Ginsenoside F2 induces the release of mediators associated with anaphylactoid reactions. Fitoterapia. 121:223-228. https://doi.org/10.1016/j.fitote.2017.07.010
  23. Wang Y, Choi KD, Yu H, Jin F and Im WT. (2016). Production of ginsenoside F1 using commercial enzyme cellulase KN. Journal of Ginseng Research. 40:121-126. https://doi.org/10.1016/j.jgr.2015.06.003
  24. Wang Y, Dong J, Liu P, Lau CW, Gao Z, Zhou D, Tang J, Ng CF and Huang Y. (2014). Ginsenoside Rb3 attenuates oxidative stress and preserves endothelial function in renal arteries from hypertensive rats. British Journal of Pharmacology. 171:3171-3181. https://doi.org/10.1111/bph.12660
  25. Woo YH, Nam YI, Song CH, Kim HJ and Kim DE. (1994). Studies on management of effective temperature and humidity in greenhouse at summer season. Journal of Biological Production Facilities and Environmental Control. 3:58-65.
  26. Yokozawa T, Kobayashi T, Oura H and Kawashima Y. (1985). Studies on the mechanism of the hypoglycemic activity of ginsenoside-$Rb_2$ in streptozotocin-diabetic rats. Chemical and Pharmaceutical Bulletin. 33:869-872. https://doi.org/10.1248/cpb.33.869
  27. Yu JL, Dou DQ, Chen XH, Yang HZ, Guo N and Cheng GF. (2004). Immunoenhancing activity of protopanaxatriol-type ginsenoside-F3 in murine spleen cells. Acta Pharmacologica Sinica. 25:1671-1676.
  28. Yu T, Yang Y, Kwak YS, Song GG, Kim MY, Rhee MH and Cho JY. (2017). Ginsenoside Rc from Panax ginseng exerts anti-inflammatory activity by targeting TANK-binding kinase 1/ interferon regulatory factor-3 and p38/ATF-2. Journal of Ginseng Research. 41:127-133. https://doi.org/10.1016/j.jgr.2016.02.001
  29. Zhang X, Wang Y, Ma C, Yan Y, Wang X and Rausch WD. (2016). Ginsenoside Rd and ginsenoside Re offer neuroprotection in novel model of Parkinson's disease. American Journal of Neurodegenerative Disease. 5:52-61.