Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Kaposi's Sarcoma-Associated Herpesvirus Infection Modulates the Proliferation of Glioma Stem-Like Cells

  • Jeon, Hyungtaek (Department of Microbiology and Immunology, Eulji University School of Medicine) ;
  • Kang, Yun Hee (Eulji Biomedical Science Research Institute, Eulji University School of Medicine) ;
  • Yoo, Seung-Min (Department of Microbiology and Immunology, Eulji University School of Medicine) ;
  • Park, Myung-Jin (Divisions of Radiation Cancer Research, Research Center for Radio-Senescence, Korea Institute of Radiological and Medical Sciences) ;
  • Park, Jong Bae (Department of System Cancer Science, Graduate School of Cancer Science and Policy, National Cancer Center) ;
  • Lee, Seung-Hoon (Department of Neurosurgery, Eulji University School of Medicine) ;
  • Lee, Myung-Shin (Department of Microbiology and Immunology, Eulji University School of Medicine)
  • Received : 2017.09.01
  • Accepted : 2017.09.18
  • Published : 2018.01.28
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Abstract

Glioblastoma multiforme is the most lethal malignant brain tumor. Despite many intensive studies, the prognosis of glioblastoma multiforme is currently very poor, with a median overall survival duration of 14 months and 2-year survival rates of less than 10%. Although viral infections have been emphasized as potential cofactors, their influences on pathways that support glioblastoma progression are not known. Some previous studies indicated that human Kaposi's sarcoma-associated herpesvirus (KSHV) was detected in healthy brains, and its microRNA was also detected in glioblastoma patients' plasma. However, a direct link between KSHV infection and glioblastoma is currently not known. In this study, we infected glioblastoma cells and glioma stem-like cells (GSCs) with KSHV to establish an in vitro cell model for KSHV-infected glioblastoma cells and glioma stem-like cells in order to identify virologic outcomes that overlap with markers of aggressive disease. Latently KSHV-infected glioblastoma cells and GSCs were successfully established. Additionally, using these cell models, we found that KSHV infection modulates the proliferation of glioma stem-like cells.

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References

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