Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biotransformation of Pueraria lobata Extract with Lactobacillus rhamnosus vitaP1 Enhances Anti-Melanogenic Activity

  • Kwon, Jeong Eun (Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Lee, Jin Woo (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Park, Yuna (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Choung, Eui Su (DanjoungBio Co., Ltd.) ;
  • Jang, Seon-A (Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Kim, Inhye (Genencell Co., Ltd.) ;
  • Lee, Da Eun (Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Koo, Hyun Jung (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Bak, Jong Phil (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Lee, Sung Ryul (Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University) ;
  • Kang, Se Chan (Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University)
  • Received : 2017.07.20
  • Accepted : 2017.10.18
  • Published : 2018.01.28
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Abstract

Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at $37^{\circ}C$. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to $500{\mu}g/ml$ of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL ($100{\mu}g/ml$) could highly suppress the content of melanin and melanosome formation in B16-F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

Keywords

References

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