Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative and antimelanogenic effects of ethyl acetate fractions of Korean domestic honeys from different floral sources

화종에 따른 국내산 벌꿀 에틸 아세테이트 분획물의 산화방지능 및 멜라닌 생성 억제 효과

  • Jeong, Ha-Ram (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Baek, Youngsu (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University)
  • 정하람 (경희대학교 식품생명공학과) ;
  • 백영수 (경희대학교 식품생명공학과) ;
  • 김대옥 (경희대학교 식품생명공학과) ;
  • 이형재 (단국대학교 식품공학과)
  • Received : 2019.08.30
  • Accepted : 2018.11.12
  • Published : 2018.12.31
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Abstract

Honey is known to have a variety of health-promoting effects. Ethyl acetate fractions (EAFs) of four Korean domestic honeys from basswood, Korean raisin, chestnut, and acacia as well as a New Zealand manuka honey were analyzed comparatively to evaluate their antioxidative and antimelanogenic effects. The EAFs of basswood, Korean raisin, and chestnut honey had higher antioxidant capacities and tyrosinase inhibition activities than those of manuka honey. Pretreatment of B16F1 melanoma cells with EAFs at $100{\mu}g/mL$ resulted in relative retention of melanin contents as follows: acacia honey (141.0%)>basswood honey (134.3%)>manuka honey (131.5%)>Korean raisin honey (107.3%)>chestnut honey (88.0%). Intracellular tyrosinase activities of B16F1 melanoma cells were significantly (p<0.05) lowered by EAFs of Korean raisin and chestnut honey than by EAF of manuka honey. Consequently, these results suggest that Korean domestic honeys from different floral sources serve as good sources of antioxidative and antimelanogenic agents.

본 연구에서 이용한 피나무 꿀, 헛개나무 꿀, 밤나무 꿀의 EAF는 마누카 꿀 EAF 보다 높은 산화방지능 및 타이로시네이스 억제능을 보였다. 특히 밤나무 꿀의 EAF는 양성대조군인 고지산에 버금가는 타이로시네이스 억제능을 보였다. 5가지 화종 유래 꿀의 EAF ($100{\mu}g/mL$) 처리시 B16F1 세포에서 멜라닌 생성 억제능은 밤나무 꿀>헛개나무 꿀>마누카 꿀>피나무 꿀>아카시아 꿀의 순서대로 낮아졌다. B16F1 세포 내 타이로시네이스의 효소활성 억제의 경우 5종류 꿀 EAF 중에서 밤나무 꿀 EAF ($100{\mu}g/mL$) 처리시 가장 높았다. 본 연구에서는 다양한 화종의 국내산 꿀에 대한 생리활성소재 탐색을 통하여 산화방지능 및 멜라닌 생성 억제 효과를 확인하였으며, 이를 통해 미백활성 소재로서 기능성 식품 및 화장품 산업으로의 적용 가능성을 제시하였다.

Keywords

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Fig. 1. Antioxidant capacities of the ethyl acetate fractions from five different varieties of honey using oxygen radical absorbance capacity assay.

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Fig. 2. Tyrosinase inhibition of the ethyl acetate fractions from five different varieties of honey.

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Fig. 3. Cytotoxic effects of the ethyl acetate fractions from five different varieties of honey on B16F1 melanoma cells.

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Fig. 4. Antimelanogenic effects of the ethyl acetate fractions from five different varieties of honey on B16F1 melanoma cells.

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Fig. 5. Effects of the ethyl acetate fractions from five different varieties of honey on intracellular tyrosinase activity in B16F1 melanoma cells.

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