Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Isolation and Molecular Identification of Bacteriocin-producing Enterococci with Broad Antibacterial Activity from Traditional Dairy Products in Kerman Province of Iran

  • M, Khodaei (Department of Microbiology, Faculty of sciences, Kerman Branch, Islamic Azad University) ;
  • Sh, Soltani Nezhad (Department of Microbiology, Jiroft Branch, Islamic Azad University)
  • Received : 2017.09.12
  • Accepted : 2018.01.28
  • Published : 2018.02.28
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Abstract

One of the critical limitations to use of bacteriocins produced by lactic acid bacteria as a substitute for chemical antibiotics is the narrow spectrum of their antibacterial activity. The aim of present study was isolation and molecular identification of bacteriocin-producing enterococci with broad antibacterial spectrum. Bacteriocin-producing bacteria were isolated from native dairies in Kerman. Bacteriocins were purified by ammonium sulfate method and the effects of them were investigated on different strains of bacteria. Also, the effects of pH and heat on produced bacteriocins were investigated. High level bacteriocin-producing isolates were identified based on molecular tests. A total of 15 strains of bacteriocin-producing Enterococcus were isolated initially. Enterococcus faecium C-2 and Y-1 strains produced bacteriocins with the highest antibacterial effect. The bacteriocins were stable in pH ranges from 2 to 12 and their antibacterial activity was maintained after autoclave treatment. The maximum bactericidal effect was observed against Listeria monocytogenes and Pseudomonas aeruginosa. In conclusion, use of these bacteriocins as a substitute for chemical antibiotics is recommended.

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References

  1. Abee T, Krockel L, Hill C. 1995. Bacteriocins: modes of action and potentials in food preservation and control of food poisoning. Int J Food Microbiol 28:169-185. https://doi.org/10.1016/0168-1605(95)00055-0
  2. Acuna L, Picariello G, Sesma F, Morero RD, Bellomio A. 2012. A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria. FEBS open bio. 2:12-19. https://doi.org/10.1016/j.fob.2012.01.002
  3. Ahmadova A, Todorov SD, Choiset Y, Rabesona H, Zadi TM, Kuliyev A, De Melo Franco BDG, Chobert JM, Haertle T. 2013. Evaluation of antimicrobial activity probiotic properties and safety of wild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal cheese. Food Control 30:631-641. https://doi.org/10.1016/j.foodcont.2012.08.009
  4. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
  5. Aran HK, Biscola V, El-Ghaish S, Jaffres E, Dousset X, Pillot G, Haertle T, Chobert JM, Hwanhlem N. 2015. Bacteriocin-producing Enterococcus faecalis KT2W2G isolated from mangrove forests in southern Thailand: purification characterization and safety evaluation. Food Control 54:126-134. https://doi.org/10.1016/j.foodcont.2014.12.037
  6. Barbosa J, Borges S, Teixeira P. 2014. Selection of potential probiotic Enterococcus faecium isolated from Portuguese fermented food. Int J Food Microbiol 191:144-148. https://doi.org/10.1016/j.ijfoodmicro.2014.09.009
  7. Barraza DE, Ríos Colombo NS, Galvan AE, Acuna L, Minahk CJ, Bellomio A, Chalon MC. 2017. New insights into enterocin CRL35: mechanism of action and immunity revealed by heterologous expression in Escherichia coli. Mol Microbiol 105:922-933. https://doi.org/10.1111/mmi.13746
  8. Bowdish DM, Davidson DJ, Hancock R. 2005. A re-evaluation of the role of host defence peptides in mammalian immunity. Curr Protein Pept Sci 6:35-51. https://doi.org/10.2174/1389203053027494
  9. Chen YS, Yanagida F, Srionnual S. 2007. Characteristics of bacteriocin like inhibitory substances from dochi isolated Enterococcus faecium D081821 and D081833. Lett Appl Microbiol 44:320-325. https://doi.org/10.1111/j.1472-765X.2006.02058.x
  10. Cintas LM, Casaus MP, Herranz C, Nes IF, Hernandez PE. 2001. Review: bacteriocins of lactic acid bacteria. Food Sci Technol Int 7:281-305. https://doi.org/10.1106/R8DE-P6HU-CLXP-5RYT
  11. Cotter PD, Hill C, Ross RP. 2005. Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 3:777-788. https://doi.org/10.1038/nrmicro1273
  12. De Vuyst L, Leroy F. 2007. Bacteriocins from lactic acid bacteria: production, purification, and food applications. J Mol Microbiol Biotechnol 13:194-199. https://doi.org/10.1159/000104752
  13. Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  14. Hadji-Sfaxi I, El-Ghaish S, Ahmadova A, Batdorj B, Le Blay-Laliberte G, Barbier G, Haertlé T, Chobert JM. 2011. Bacillus subtilis strain 14B for biocontrol of Agrobacterium spp. strains. Lett Appl Microbiol 48:253-260.
  15. Javed A, Masud T, ul Ain Q, Imran M, Maqsood S. 2011. Enterocins of Enterococcus faecium, emerging natural food preservatives. Ann Microbiol 61:699-708. https://doi.org/10.1007/s13213-011-0223-8
  16. Khan H, Flint S, Yu PL. 2010. Enterocins in food preservation. Int J Food Microbiol 141:1-10. https://doi.org/10.1016/j.ijfoodmicro.2010.03.005
  17. Nilsen T, Nes IF, Holo H. 2003. Enterolysin A: a cell wall-degrading bacteriocin from Enterococcus faecalis LMG 2333. Appl Environ Microbiol 69:2975-2984. https://doi.org/10.1128/AEM.69.5.2975-2984.2003
  18. Kang JH, Lee MS. 2005. Characterization of a bacteriocin produced by Enterococcus faecium GM-1 isolated from an infant. J Appl Microbiol 98:1169-76. https://doi.org/10.1111/j.1365-2672.2005.02556.x
  19. Olasupo NA, Schillinger U, Narbad A, Dodd H, Holzapfel WH. 1999. Occurrence of nisin Z production in Lactococcus lactis BFE 1500 isolated from wara, a traditional Nigerian cheese product. Int J Food Microbiol 53:141-152. https://doi.org/10.1016/S0168-1605(99)00146-4
  20. Park SH, Itoh K, Fujisawa T. 2003. Characteristics and identification of enterocins produced by Enterococcus faecium JCM 5804T. J Appl Microbiol 95:294-300. https://doi.org/10.1046/j.1365-2672.2003.01975.x
  21. Rehaiem A, Martinez B, Manai M, Rodriguez A. 2010. Production of enterocin A by Enterococcus faecium MMRA isolated from 'Rayeb', a traditional Tunisian dairy beverage. J Appl Microbiol 108:1685-1693. https://doi.org/10.1111/j.1365-2672.2009.04565.x
  22. Rodriguez E, Arques JL, Rodriguez R, Peiroten A, Landete JM, Medina M. 2012. Antimicrobial properties of probiotic strains isolated from breast-fed infants. J Funct Foods 4:542-551. https://doi.org/10.1016/j.jff.2012.02.015
  23. Saranya S, Hemashenpagam N. 2011. Antagonistic activity and antibiotic sensitivity of lactic acid bacteria from fermented dairy products. Adv Appl Sci Res 2:528-534.
  24. Settanni LU, Massitti O, Van Sinderen D, Corsetti A. 2005. In situ activity of a bacteriocin producing Lactococcus lactis strain. Influence on the interactions between lactic acid bacteria during sourdough fermentation. J Appl Microbiol 99:670-681. https://doi.org/10.1111/j.1365-2672.2005.02647.x
  25. Shokri D, Zaghian S, Khodabakhsh F, Fazeli H, Mobasherizadeh S, Ataei B. 2014. Antimicrobial activity of a UV-stable bacteriocin-like inhibitory substance (BLIS) produced by Enterococcus faecium strain DSH20 against vancomycin-resistant Enterococcus (VRE) strains. J Microbiol Immunol Infect 47:371-376. https://doi.org/10.1016/j.jmii.2013.05.004
  26. Sonsa-Ard N, Rodtong S, Chikindas ML, Yongsawatdigul J. 2015. Characterization of bacteriocin produced by Enterococcus faecium CN-25 isolated from traditionally Thai fermented fish roe. Food Control 54:308-316. https://doi.org/10.1016/j.foodcont.2015.02.010
  27. Sun Y, Lou X, Zhu X, Jiang H, Gu Q. 2014. Isolation and characterization of lactic acid bacteria producing bacteriocin from newborn infant's feces. J Bacteriol Mycol 1:1-7.
  28. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony Methods. Mol Biol Evol 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
  29. Todorov SD, Wachsman M, Tome E, Dousset X, Destro MT, Dicks LM, Franco BD, Vaz-Velho M, Drider D. 2010. Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. Food Microbiol 27: 869-879. https://doi.org/10.1016/j.fm.2010.05.001
  30. Yang E, Fan L, Jiang Y, Doucette C, Fillmore S. 2012. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2:1-12. https://doi.org/10.1186/2191-0855-2-1
  31. Zhang QQ, Ying GG, Pan CG, Liu YS, Zhao JL. 2015. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance. Environ Sci Technol 49:6772-6782. https://doi.org/10.1021/acs.est.5b00729

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