Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Development of Hydrogel for Decrease Protein Adsorption and Application to Intraocular Lens

단백질흡착 감소용 하이드로겔의 개발 및 인공수정체로의 적용

  • Ko, Na Young (Dept. of Optometry & Vision Science, Catholic University of Daegu) ;
  • Lee, Hyun Mee (Dept. of Optometry & Vision Science, Catholic University of Daegu)
  • 고나영 (대구가톨릭대학교 안경광학과) ;
  • 이현미 (대구가톨릭대학교 안경광학과)
  • Received : 2017.10.12
  • Accepted : 2017.12.23
  • Published : 2018.02.20
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Abstract

The purpose of this study was to decrease the protein adsorption and improve the function of the hydrophobic acrylic Intraocular lens(IOL). Hydrophobic acrylic intraocular lenses were prepared by using ethyleneglycol phenyletheracrylate (EGPEA), styrene and 2-hydroxyethyl methacrylate (HEMA). Polyvinyl pyrrolidone (PVP) and 2-methacryloyloxyethyl phosphorylcholine (MPC) were used as additives. Water contents, wettability, light transmittance and protein adsorption amount were measured to evaluate the physical properties of the intraocular lens. The water content and wettability of all samples containing additives were increased and the amount of protein adsorption decreased. In particular, samples containing MPC showed a further decrease in protein adsorption. The hydrophobic acrylic intraocular lens with PVP and MPC was found to improve the function of the intraocular lens by reducing the protein adsorption while having basic physical properties.

인공수정체로 사용되고 있는 소수성 아크릴 하이드로겔의 단백질 흡착 감소와 기능을 향상시키기 위한 하이드로겔을 제조하여 인공수정체로의 응용성을 살펴보고자 한다. 소수성 아크릴 인공수정체는 ethyleneglycol phenyletheracrylate(EGPEA), Styrene, 2-hydroxyethyl methacrylate(HEMA)를 사용하여 제작하였고 첨가제로 polyvinyl pyrrolidone(PVP)와 2-methacryloyloxyethyl phosphorylcholine(MPC)를 사용하였다. 인공수정체의 물리적 특성을 평가하기 위해 함수율, 습윤성, 광투과율 및 단백질 흡착량을 측정하였다. 첨가제를 넣은 모든 시료의 함수율과 습윤성은 증가하였고 단백질 흡착량은 감소하였다. 특히 MPC가 포함된 시료의 단백질 흡착량은 더욱 감소하였다. PVP와 MPC를 첨가한 소수성 아크릴 하이드로겔은 기본적인 물리적 특성을 가지면서 단백질 흡착을 감소시킴으로써 기능이 향상된 인공수정체로의 응용이 가능하다는 것을 확인하였다.

Keywords

References

  1. Apple, D. J.; Sims, J. Survey of Ophthalmology 1996, 40, 279. https://doi.org/10.1016/S0039-6257(96)82003-0
  2. Alio, J. L.; Chipont, E.; BenEzra, D.; Fakhry, M. A. Cataract & Refractive Surgery 2002, 28, 2096. https://doi.org/10.1016/S0886-3350(02)01452-9
  3. Ronbeck, M.; Kugelberg, M. Cataract & Refractive Surgery 2010, 40, 70.
  4. Bellucci, R. Cataract & Refractive Surgery 2013, 3, 38.
  5. Seward, H. C. British Journal of Ophthalmology 1997, 81, 340. https://doi.org/10.1136/bjo.81.5.340
  6. Hollick, E. J.; Spalton, D. J.; Ursell, P. G.; Meacock, W. R.; Barman, S. A.; Boyce, J. F. American Journal of Ophthalmology 2000, 129, 577. https://doi.org/10.1016/S0002-9394(99)00447-X
  7. Werner, L.; Mamalis, N. Cataract & Refractive Surgery 2005, 63.
  8. Aasuri, M. K.; Fernandes, M.; Pathan, P. P. Indian Journal of Ophthalmology 2006, 54, 105. https://doi.org/10.4103/0301-4738.25831
  9. Leaming, D. V. Cataract & Refractive Surgery 2004, 30, 892 https://doi.org/10.1016/j.jcrs.2004.02.064
  10. Findl, O. Achieving Excellence in Cataract Surgery 2009, 12, 95.
  11. Nakanome, S.; Watanabe, H.; Tanaka, K.; Tochikubo, T. Cataract & Refractive Surgery 2008, 34, 80. https://doi.org/10.1016/j.jcrs.2007.08.020
  12. Marcia, D.; Callaghan, T. A.; Pei, R.; Karakelle, M. Cataract & Refractive Surgery 2012, 38, 1833. https://doi.org/10.1016/j.jcrs.2012.05.043
  13. Saeed, M. U.; Jafree, A. J.; Saeed, M. S.; Zia, R.; Sheikh, I. M.; Heravi, M. Seminars in Ophthalmology 2012, 27, 15. https://doi.org/10.3109/08820538.2011.588651
  14. Nanavaty, M. A.; Spalton, D. J.; Boyce, J.; Brain, A.; Marshall, J. Cataract & Refractive Surgery 2008, 34, 677. https://doi.org/10.1016/j.jcrs.2007.12.024
  15. Auffarth, G.; Brezin, A.; Caporossi, A.; Lafuma, A.; Mendicute, J.; Berdeaux, G.; Smith, A. Ophthalmic Epidemiology 2004, 11, 319. https://doi.org/10.1080/09286580490515116
  16. Li, Y.; Wang, J.; Chen, Z.; Tang, X. PLoS ONE 2013, 8, e77864. https://doi.org/10.1371/journal.pone.0077864
  17. Yuan, Z. Graefe's Archive for Clinical and Experimental Ophthalmology 2003, 241, 840. https://doi.org/10.1007/s00417-003-0728-3
  18. Huang, X. D.; Yao, K.; Zhang, Z.; Zhang, Y.; Wang, Y. Cataract & Refractive Surgery 2010, 36, 290. https://doi.org/10.1016/j.jcrs.2009.09.027
  19. Li, L.; Wang, J. H.; Xin, Z. European Polymer Journal 2011, 47, 1795. https://doi.org/10.1016/j.eurpolymj.2011.06.011
  20. Jones, L.; Mann, A.; Evans, K.; Franklin, V.; Tighe, B. Optometry & Vision Science 2000, 77, 503. https://doi.org/10.1097/00006324-200010000-00004
  21. Zirm, M. Advances in Ophthalmology and Optometry 1980, 40, 100.
  22. Johnston, R. L.; Spalton, D. J.; Hussain, A.; Marshall, J. Cataract & Refractive Surgery 1999, 25, 1109. https://doi.org/10.1016/S0886-3350(99)00137-6
  23. Shah, S. M.; Spalton, D. J.; Taylor, J. C. Investigative Ophthalmology & Visual Science 1992, 33, 2878.
  24. Luensmann, D.; Heynen, M.; Liu, L.; Sheardown, H.; Jones, L. Cataract & Refractive Surgery 2009, 35, 2000. https://doi.org/10.1016/j.jcrs.2009.05.052
  25. Bae, S. E.; Park, K.; Han, D. K. Biomaterials Research 2008, 12, 167.
  26. Tognetto, D.; Ravalico, G. Cataract & Refractive Surgery 2001, 27, 239. https://doi.org/10.1016/S0886-3350(00)00603-9
  27. Tighe, B.; Franklin, V. Butterworth Heinemann 1985, 49.
  28. Santos, L.; Rodrigues, D.; Lira, M.; Oliveira, M. E.; Oliveira, R.; Vilar, E. Y.; Azeredo, J. Contact Lens & Anterior Eye 2007, 30, 183. https://doi.org/10.1016/j.clae.2006.12.007
  29. Luensmann, D.; Jones, L. Contact Lens & Anterior Eye 2012, 35, 53. https://doi.org/10.1016/j.clae.2011.12.005
  30. Viveiros, M. M.; Soares, R. T.; Omodei, M. S.; Rainho, C. A.; Padovani, C. R.; Cruz, N.; Schellini, S. A.; Rodrigues, A. C. Cataract & Refractive Surgery 2015, 41, 1478. https://doi.org/10.1016/j.jcrs.2014.11.047
  31. Kanaze, F. I.; Kokkalou, E.; Niopas, I.; Barmpalexis, P.; Georgarakis, E.; Bikiaris, E. Drug Development and Industrial Pharmacy 2007, 36, 292.
  32. Nair, B. International Journal of Toxicology 1998, 17, 95. https://doi.org/10.1177/109158189801700408
  33. Chun, Y. S. Journal of the Korean Medical Association 2007, 50, 842. https://doi.org/10.5124/jkma.2007.50.9.842
  34. Liu, Z. M.; Xu, Z. K.; Wan, L. S.; Wu, J.; Ulbricht, M. S. Journal of Membrane Science 2005, 249, 21. https://doi.org/10.1016/j.memsci.2004.10.001
  35. Pieracci, J.; Crivello, J. V.; Belfort, G. Journal of Membrane Science 1999, 156, 223. https://doi.org/10.1016/S0376-7388(98)00347-0
  36. Goda, T.; Matsuno, R.; Konno, T.; Takai, M.; Ishihara, K. Colloids and Surfaces B: Biointerfaces 2008, 63, 64. https://doi.org/10.1016/j.colsurfb.2007.11.014
  37. Iwasaki, Y.; Aiba, Y.; Morimoto, N.; Nakabayashi, N.; Ishihara, K. Journal of Biomedical Materials Research 2000, 52, 701. https://doi.org/10.1002/1097-4636(20001215)52:4<701::AID-JBM15>3.0.CO;2-6
  38. Lewis, A. L. Colloids and Surfaces B: Biointerfaces 2000, 18, 261. https://doi.org/10.1016/S0927-7765(99)00152-6
  39. Sibarani, J. Colloids and Surfaces B: Biointerfaces 2007, 54, 88. https://doi.org/10.1016/j.colsurfb.2006.09.024
  40. Ishihara, K. Science and Technology of Advanced Materials 2000, 1, 131. https://doi.org/10.1016/S1468-6996(00)00012-7
  41. Hirota, K.; Murakami, K.; Nemoto, K.; Miyake, Y. FEMS Microbiology Letters 2005, 248, 37. https://doi.org/10.1016/j.femsle.2005.05.019
  42. Moro, T.; Takatori, Y.; Ishihara, K.; Konno, T.; Takigawa, Y.; Matsushita, T.; Chung, U. I.; Nakamura, K.; Kawaguchi, H. Nature Materials 2004, 3, 829. https://doi.org/10.1038/nmat1233
  43. Selan, L.; Palma, S.; Scoarughi, G. L.; Papa, R.; Veeh, R.; Clemente, D.; Artini, M. American Journal of Ophthalmology 2009, 147, 134. https://doi.org/10.1016/j.ajo.2008.07.032
  44. Gao, B.; Hu, H.; Guo, J.; Le, Y. Colloids and Surfaces B: Biointerfaces 2010, 77, 206. https://doi.org/10.1016/j.colsurfb.2010.01.025
  45. Cho, S. A.; Kim, T. H.; Sung, A. Y. Journal of the Korean Chemical Society 2011, 55. 283. https://doi.org/10.5012/jkcs.2011.55.2.283
  46. Kim, M. S.; Khang, G.; Lee, J. H.; Lee, H. B. Biomaterials Research 2006, 10, 125.
  47. Park, M. S.; Kwon, M. J.; Lee, S. H.; Kim, D. S. Journal of Korean Ophthalmic Optics Society 2004, 9, 53.