대한약침학회지 (Journal of Pharmacopuncture)

  • 제21권4호
  • /
  • Pages.249-257
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  • 2018
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  • 2093-6966(pISSN)
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  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
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The Preventive Effects of Standardized Extract of Zataria multiflora and Carvacrol on Acetaminophen-Induced Hepatotoxicity in Rat - Zataria multiflora and Carvacrol and Hepatotoxicity -

  • Mohebbati, Reza (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Paseban, Maryam (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Beheshti, Farimah (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Soukhtanloo, Mohammad (Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Shafei, Mohammad Naser (Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences) ;
  • Rakhshandeh, Hasan (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences) ;
  • Rad, Abolfazl Khajavi (Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences)
  • 투고 : 2018.10.11
  • 심사 : 2018.11.14
  • 발행 : 2018.12.31
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초록

Objectives: The hepatotoxicity induced by Acetaminophen (AAP) mostly mediated by effect on oxidative stress parameters. The Zataria multiflora (Z.M) is an herbal medicine with well-known antioxidant effect. The aim of this study is investigation of preventive effects of Z.M and Carvacrol (CAR) on AAP-induced hepatotoxicity in rats. Methods: Rats were randomly divided into four groups including: 1) Control, 2) Acetaminophen (AAP), 3) and 4) CAR. The saline, Z.M (200 mg/kg) and CAR (20 mg/kg) were administrated orally for 6 days, after that AAP (600 mg/kg) was administrated in the $7^{th}$ day. Blood sampling was performed on the first and last days. Also, the liver tissue was removed for evaluation of Malondyaldehide (MDA), Thiol content, Superoxide dismutase (SOD) and Catalase (CAT). Total Protein (tPro), Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT) and Alkaline Phosphatase (ALP) in liver tissue were evaluated. The changes (${\Delta}$) of enzymes activities were presented. Results: The ${\Delta}GOT$, ${\Delta}GPT$ and ${\Delta}ALP$ in CAR group significantly decreased compared to AAP group (P < 0.01 to P < 0.001) and ${\Delta}GPT$ in Z.M group was significantly reduced in comparison with AAP group (P < 0.05). Also, MDA, Thiol, SOD and CAT levels in treated groups were attenuated compared to AAP group (P < 0.05 to P < 0.001). Conclusion: Z.M and CAR have a powerful hepatoprotective effect. CAR is more effective than Z.M. Based on the results. Z.M and CAR could be potent supplementary agents against hepatotoxicity of AAP in patients.

키워드

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Figure 2 Metabolic and hepatotoxicity pathways of AAP [28]

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Figure 2 Metabolic and hepatotoxicity pathways of AAP [28]

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Figure 1 Acetaminophen structure [28]

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Figure 1 Acetaminophen structure [28]

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Figure 3 Carvacrol structure [29]

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Figure 3 Carvacrol structure [29]

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Figure 4 Changes (Δ) of different parameters induced by AAP group in comparison with Co group (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 4 Changes (Δ) of different parameters induced by AAP group in comparison with Co group (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 5 Comparison the changes (Δ) of Glutamic Oxaloacetic Transaminase (GOT) in four experimental groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 5 Comparison the changes (Δ) of Glutamic Oxaloacetic Transaminase (GOT) in four experimental groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 6 Comparison the changes (Δ) of Glutamic Pyruvic Transaminase (GPT) between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 6 Comparison the changes (Δ) of Glutamic Pyruvic Transaminase (GPT) between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 7 Comparison the changes (Δ) of Alkaline Phosphatase (ALP) concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 7 Comparison the changes (Δ) of Alkaline Phosphatase (ALP) concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 8 Comparison the changes (Δ) of tPro between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 8 Comparison the changes (Δ) of tPro between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 9 Comparison of Malondyaldehide (MDA) concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 9 Comparison of Malondyaldehide (MDA) concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 11 Comparison of CAT activity between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 11 Comparison of CAT activity between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 12 Comparison of Superoxide dismutase (SOD) activity between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 12 Comparison of Superoxide dismutase (SOD) activity between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

DHOCBS_2018_v21n4_249_f0012.png 이미지

Figure 10 Comparison of Thiol concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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Figure 10 Comparison of Thiol concentration between four groups (n=6 in each group). Values are the Mean ± SEM. The data were analyzed using One-Way ANOVA with tukey post hoc.

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