대한한의학방제학회지 (Herbal Formula Science)

  • 제26권4호
  • /
  • Pages.295-306
  • /
  • 2018
  • /
  • 1229-1218(pISSN)
  • /
  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
권호 표지
DOI QR코드

DOI QR Code

포제 및 발효 가공에 따른 오미자와 구기자 물 추출물의 항염증 및 숙취해소 효과

Anti-inflammation and hangover relief effects of Schisandra chinensis (SC) and Lycium chinense (LC) water extracts depending on drug processing and fermentation

  • 김하림 ((재)전주농생명소재연구원) ;
  • 김상준 ((재)전주농생명소재연구원) ;
  • 김솔 ((재)전주농생명소재연구원) ;
  • 김홍준 (우석대학교 한의과대학) ;
  • 정승일 ((재)전주농생명소재연구원) ;
  • 유강열 ((재)전주농생명소재연구원) ;
  • 김선영 ((재)전주농생명소재연구원)
  • 투고 : 2018.11.12
  • 심사 : 2018.11.28
  • 발행 : 2018.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Schisandra chinensis (SC) and Lycium chinense (LC) were widely distributed in Asia and the fruit has been used traditionally for medicinal herbs. The processing method was solid-state fermentation using Aspergillus oryzae for 48 h after stir-frying treatment at $220^{\circ}C$ for 12 min. In this study, in vitro the anti-inflammatory effect and in vivo hangover reduction were compared to unprocessed SC and LC water extract. Anti-inflammatory effects have been evaluated in pro-inflammatory mediators which were secreted by lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Nitric oxide (NO) was determined using Griess reaction. Proinflammatory cytokines such as tumor necrosis factor $(TNF)-{\alpha}$ and interleukin $(IL)-1{\beta}$ were measured by enzyme-linked immunosorbent assays (ELISA). Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were compared to processed SC or LC and mixtures thereof (1:1). In vivo study was compared to hangover relief in alcohol-fed mice. After administering a mixture of SC and LC (300 mg/kg) water extract (1:1), mice were fed 3 g/kg of ethanol. Serum was collected at 1, 3, and 5 h intervals to analyze ethanol and acetaldehyde levels using a colorimetric assay kit. The processed SC and LC water extracts compared to raw materials significantly inhibited LPS-induced NO and inflammatory cytokine production in RAW 264.7 cells. The results of the hangover mouse model are also consistent with anti-inflammatory effects. These results suggest that processed SC and LC extracts may be functional materials for the treatment of inflammation and hangover.

키워드

HBBJBB_2018_v26n4_295_f0002.png 이미지

Fig. 1. Inhibitory effects of processed Schisandra chinensis (SC) and Lycium chinense (LC) on nitric oxide (NO) and inflammatory cytokines production in RAW 264.7 cell.

HBBJBB_2018_v26n4_295_f0003.png 이미지

Fig. 2. Comparison results for the processed SC and LC products against on Alcohol dehydrogenase (ADH) and Aldehyde dehydrogenase (ALDH) activation.

HBBJBB_2018_v26n4_295_f0004.png 이미지

Fig. 3. Effects of processed SC and LC mixture on relieving hangover in mice administered alcohol.

Table 1. The classification of group

HBBJBB_2018_v26n4_295_t0001.png 이미지

Table 2. ABTS radical scavenging activity, DPPH radical scavenging activity and SOD activity of Schisandra chinensis (SC) and Lycium chinense (LC)

HBBJBB_2018_v26n4_295_t0002.png 이미지

Table 3. Effects of processed SC and LC mixture on serum ADH activity.

HBBJBB_2018_v26n4_295_t0003.png 이미지

참고문헌

  1. TSUKAMOTO S, MUTO T, NAGOYA T, SHIMAMURA M, SAITO M, TAINAKA H. Determinations of ethanol, acetaldehyde and acetate in blood and urine during alcohol oxidation in man. Alcohol and Alcoholism. 1989;24:101-08. https://doi.org/10.1093/oxfordjournals.alcalc.a044872
  2. Tsukamoto S, Kanegae T, Uchigasaki S, Kitazawa M, Fujioka T, Fujioka S, Imamura Y, Nagoya T, Shimamura M, Mieda Y. Changes in free and bound alcohol metabolites in the urine during ethanol oxidation. Arukoru Kenkyu to Yakubutsu Izon 1993;28:441-52.
  3. Xu BJ, Xu BJ, Zheng YN, Xu BJ, Zheng YN, Sung CK, Sung CK. Natural medicines for alcoholism treatment:a review. Drug Alcohol Abuse Rev. 2005;24:525-36. https://doi.org/10.1080/09595230500293795
  4. Lieber CS. Medical disorders of alcoholism. N. Engl. J. Med. 1995;333:1058-65. https://doi.org/10.1056/NEJM199510193331607
  5. Lieber CS, Garro A, Leo MA, Mak KM, Worner T. Alcohol and cancer. Hepatology 1986; 6:1005-19. https://doi.org/10.1002/hep.1840060533
  6. Forsander OA, Raiha NC. Metabolites produced in the liver during alcohol oxidation. J Biol Chem. 1960;235:34-36.
  7. Mezey E. Alcoholic liver disease:roles of alcohol and malnutrition. Am J Clin Nutr 1980; 33:2709-18. https://doi.org/10.1093/ajcn/33.12.2709
  8. Lieber C-S, DeCarli L-M. Hepatic microsomal ethanol-oxidizing system in vitro characteristics and adaptive properties in vivo. J. Biol. Chem. 1970;245:2505-12.
  9. Ju Y-S. Ungok Herbology. Jeonju:Woosuk Press 2013;819:826-8.
  10. Kim M-J, Park E-J. Antioxidative and antigenotoxic effect of omija (Schizandra chinensis B.) extracted with various solvents. Korean J Crop Sci 2010;39:487-93.
  11. Kim K, Park C, Ryu S, Bang J, Lee BJ. Schizandrin, oil compounds, and their extraction yield in fruits of Schizandra chinensis Baillon. 2000;45:158-62.
  12. Koo D-C, Suh W-S, Baek S-Y, Shim S-H. Quantitative determination of lignans from Schizandra chinensis by HPLC. Korean J. org. Agric 2011.
  13. Lee S, Moon H-K, Lee S-W, Moon J-N, Kim J-K. Effects of drying methods on quality characteristics and antioxidative effects of Omija (Schizandra chinesis bailon). Korean J Food Preserv 2014;21:341-49. https://doi.org/10.11002/kjfp.2014.21.3.341
  14. Lee B-Y, Kim E-J, Choi H-D, Kim Y-S, Kim I-H, Kim S-S. Physico-chemical properties of Boxthorn (Lycii fructus) hot water extracts by roasting conditions. KOREAN J. FOOD SCI. TECHNOL 1995;27: 768-72.
  15. Park U-Y, Chang D-S, Cho H-R. Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr 1992;21:91-96.
  16. Lim D-K, Choi U, Shin D-H. Antioxidative activity of ethanol extract from Korean medicinal plants. KOREAN J. FOOD SCI. TECHNOL 1996;28:83-89.
  17. Cho E-H, Roh S-S, Seo Y-B, Jeong G-HJTKJoH. The Comparative Study of Quality by Processing Methods of Liriope platyphylla. Kor. J. Herbology 2010;25:95-102.
  18. Oh J-H, Sin J-S, Ahn E-S, Lee S-J, Lee J-c, Lim J-h, Hong S-k, Hong J-k, Lee Y-JJJoPI. A Literature Survey of the Modern Techniques Used for the Processing of Herbal Medicines. J. Kor. Pharm. Sci 2009;39:275-297.
  19. Nam H-H, Kim H-J, Choi N-J, Roh S-S, Choo B-K. A Comparison of Antioxidant Activity from Schisandra chinensis Water Extracts depending on Stir-frying and Stir-frying with Liquids Process. Korean J. org. Agric 2015;23:987-997. https://doi.org/10.11625/KJOA.2015.23.4.987
  20. Lee, S-I, Lee, Y-K, Kim, S-D, Yang, S-H, Suh, J-W. Sensory quality, antioxidant, and inhibitory activities of XO and AO of Smilax china leaf tea fermented by Aspergillus oryzae. Korean J. Food Preserv 2014;21:129-139. https://doi.org/10.11002/kjfp.2014.21.1.129
  21. Kang, Y-H, Kim, K-K, Kim, T-W, Yang, C-S, Choe, M. Evaluation of the anti-obesity activity of Platycodon grandiflorum root and Curcuma longa root fermented with Aspergillus oryzae. Korean J. Food SCI. TECHNOL 2015; 47:111-118. https://doi.org/10.9721/KJFST.2015.47.1.111
  22. Sung, H, Lee, Y-H, Jun, W. In Vitro Hepatoprotective Effects of Fermented Curcuma longa L. by Aspergillus oryzae against Alcohol-Induced Oxidative Stress. J Korean Soc Food Sci Nutr 2016; 45:812-818.
  23. Engwall E, Perlmann PJJI. Enzyme-linked immunossorbent assay, ELISA. 3. Quantitation of specific antibodies by enzyme-labelled anti-immunglobulin in antigen-coated tubes. J Immunol 1972;109:129-35.
  24. Tezuka Y, Irikawa S, Kaneko T, Banskota AH, Nagaoka T, Xiong Q, Hase K, Kadota S. Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bungeanum. J Ethnopharmacol 2001;77:209-17. https://doi.org/10.1016/S0378-8741(01)00300-2
  25. Delgado AV, McManus AT, Chambers JPJN. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides 2003; 37:355-61. https://doi.org/10.1016/j.npep.2003.09.005
  26. Casey LC, Balk RA, Bone RC. Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome. Ann Intern Med 1993;119:771-8. https://doi.org/10.7326/0003-4819-119-8-199310150-00001
  27. Shumate R, Crowther R, Zarafshan MJJofm. A study of the metabolism rates of alcohol in the human body. J. Forensic Med 1967;14:83.
  28. Lieber CSJNEJoM. Liver adaptation and injury in alcoholism. N. Engl. J. Med 1973;288:356-62. https://doi.org/10.1056/NEJM197302152880710