Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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The Protective Effects of Mahaengeuigam-Tang against Monosodium Iodoacetate induced Osteoarthritis in Rats

마행의감탕(麻杏薏甘湯)이 골관절염 유발 흰쥐의 apoptosis 기전에 미치는 영향

  • Kim, Bum Hoi (Department of Anatomy, College of Korean Medicine and Research Institute of Korean Medicine, Dong-eui University)
  • 김범회 (동의대학교 한의과대학 한의학과, 동의대학교 한의학연구소)
  • Received : 2018.10.24
  • Accepted : 2018.11.20
  • Published : 2018.11.30
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Abstract

Objectives : Mahaengeuigam-Tang (MHEGT) has been used as a traditional medicine for the treatment of rheumatic aerthritis, rheumatisim, eczema and asthma. The aim of this study was to investigate the molecular mechanisms of MHEGT for cartilage protection in monosodium iodoacetate(MIA)-induced osteoarthritis, particularly focusing on apoptosis. Method : Thirty young male Sprague-Dawley rats were used for the study. Rats were intra-articularly injected with 2 mg MIA in a total volume of 50 ㎕ saline. In MHEGT group, MHEGT extract was orally administered once daily to MIA-induced osteoarthritis rats, and rats of control group were given with saline only. At 4 weeks after MIA injection, all animals were sacrificed, and the histological changes and articular thickness were assessed by hematoxylin and eosin staining. Moreover, the immunohistochemical analyses of BAX and Bcl-2 were carried out. Results : The histomorphological examinations revealed that MHEGT reduced MIA-induced cartilage damage. And, MHEGT ameliorated the severity of cartilage surface damages after MIA injection. Furthermore, MHEGT suppressed the MIA-induced increases of pro-apoptotic BAX protein and increased the protein expression of anti-apoptotic Bcl-2 protein. Conclusion : These findings indicate that MHEGT protects against MIA-induced cartilage damage by inhibition of the apoptotic pathway, demonstrating significant protection of cartilage against osteoarthritis. These results suggest that MHEGT may potentially have clinical applications in the treatment of osteoarthritis.

Keywords

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Fig. 1. Body weight changes of rats in normal, control, and MHEGT groups

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Fig. 2. Comparisons of gross appearance of right tibial plateau in rat of normal, control, and MHEGT groups.

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Fig. 3. The histological analyses of right articular cartilage and bone changes in the rat of normal, control, and MHEGT groups.

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Fig. 4. Quantitative assessment of right articular cartilage thickness in the rat of normal, control, and MHEGT groups.

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Fig. 5. Representative photographs of BAX immunoactivities of right articular cartilage in Rats of normal, control, and MHEGT groups.

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Fig. 6. Representative photographs of Bcl-2 immunoactivities of articular cartilage in Rats of normal, control, and MHEGT groups.

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Fig. 6. Quantitative assessment of BAX and Bcl-2 immunohistochemistry in the rat of normal, control, and MHEGT groups.

Table 1. Herbal Composition of Mahaengeuigam-Tang(MHEGT)

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Table 2. BAX and Bcl-2 Immunohistochemistry of Normal, Control, and MHEGT groups.

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