Fig. 1. Body weight changes of rats in normal, control, and MHEGT groups
Fig. 2. Comparisons of gross appearance of right tibial plateau in rat of normal, control, and MHEGT groups.
Fig. 3. The histological analyses of right articular cartilage and bone changes in the rat of normal, control, and MHEGT groups.
Fig. 4. Quantitative assessment of right articular cartilage thickness in the rat of normal, control, and MHEGT groups.
Fig. 5. Representative photographs of BAX immunoactivities of right articular cartilage in Rats of normal, control, and MHEGT groups.
Fig. 6. Representative photographs of Bcl-2 immunoactivities of articular cartilage in Rats of normal, control, and MHEGT groups.
Fig. 6. Quantitative assessment of BAX and Bcl-2 immunohistochemistry in the rat of normal, control, and MHEGT groups.
Table 1. Herbal Composition of Mahaengeuigam-Tang(MHEGT)
Table 2. BAX and Bcl-2 Immunohistochemistry of Normal, Control, and MHEGT groups.
References
- Aigner T, Kurz B, Fukui N, Sandell L. Roles of chondrocytes in the pathogenesis of osteoarthritis. Curr Opin Rheumatol. 2002;14(5):578-84. https://doi.org/10.1097/00002281-200209000-00018
- Tsujii A, Nakamura N, Horibe S. Age-related changes in the knee meniscus. Knee. 2017;24(6):1262-70. https://doi.org/10.1016/j.knee.2017.08.001
- Rodriguez-Merchan EC. Topical therapies for knee osteoarthritis. Postgrad Med. 2018;130(7):607-12. https://doi.org/10.1080/00325481.2018.1505182
- Blanco FJ, Guitian R, Vazquez-Martul E, de Toro FJ, Galdo F. Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology. Arthritis Rheum. 1998;41(2):284-9. https://doi.org/10.1002/1529-0131(199802)41:2<284::AID-ART12>3.0.CO;2-T
- Kim DY, Taylor HW, Moore RM, Paulsen DB, Cho DY. Articular chondrocyte apoptosis in equine osteoarthritis. Vet J. 2003;166(1):52-7. https://doi.org/10.1016/S1090-0233(02)00305-2
- Thomas CM, Fuller CJ, Whittles CE, Sharif M. Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis Cartilage. 2007;15(1):27-34. https://doi.org/10.1016/j.joca.2006.06.012
- Lee SI, Kim DG, Kim YP, Roh SH, Park YJ, Joo YS. Herbal Formula Science. 2nd ed. Seoul:Young Lim Sa. 1994:47.
- Moon YH, Roh EM. Anti-Inflammatory, Analgesic and Antipyretic Actions of Mahaengeuigam-Tang. Korean Journal of Pharmacognosy. 2001;32(3): 242-7.
- Lee SG, Lee EJ, Park WD, KIm JB, Choi SW. Antioxidant and Anti-inflammatory Activities of Extracts from Korean Traditional Medicinal Prescriptions. Korean Journal of Food Science and Technology. 2011;43(5):624-32. https://doi.org/10.9721/KJFST.2011.43.5.624
- Kim TH, Lee CH. Effects of Mahangeuigam-Tang on Obesity-related Factors in Brain and Gastrointestinal Tract of Mice. Journal of physiology & pathology in Korean Medicine. 2012;26(2):166-74.
- Hong JM, Shin JK, Kim JY, Jang MJ, Park SK, Lee JH, Choi JH, Lee SM. BST106 Protects against Cartilage Damage by Inhibition of Apoptosis and Enhancement of Autophagy in Osteoarthritic Rats. Biol Pharm Bull. 2018; 41(8):1257-68. https://doi.org/10.1248/bpb.b18-00207
- Oh IS, Kim MK, Lee SH, Park SW, Kwon DG. The Change of Articular Cartilage Thickness of the Knee Joint Related to Age in Korean. Knee surgery & related research. 2005;17(1):1-7.
- Mora JC, Przkora R, Cruz-Almeida Y. Knee osteoarthritis:pathophysiology and current treatment modalities. J Pain Res. 2018;11: 2189-96. https://doi.org/10.2147/JPR.S154002
- Silverwood V, Blagojevic-Bucknall M, Jinks C, Jordan JL, Protheroe J, Jordan KP. Current evidence on risk factors for knee osteoarthritis in older adults:a systematic review and meta-analysis. Osteoarthritis Cartilage. 2015; 23(4):507-15. https://doi.org/10.1016/j.joca.2014.11.019
- Pereira D, Ramos E, Branco J. Osteoarthritis. Acta Med Port. 2015;28(1):99-106.
- Burr DB, Gallant MA. Bone remodelling in osteoarthritis. Nat Rev Rheumatol. 2012; 8(11):665-73. https://doi.org/10.1038/nrrheum.2012.130
- Burr DB. The importance of subchondral bone in osteoarthrosis. Curr Opin Rheumatol. 1998;10(3):256-62. https://doi.org/10.1097/00002281-199805000-00017
- Morais SV, Czeczko NG, Malafaia O, Ribas JM Filho, Garcia JB, Miguel MT, Zini C, Massignan AG. Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee. Acta Cir Bras. 2016;31(11):765-73. https://doi.org/10.1590/s0102-865020160110000010
- Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Monoiodoacetate induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints:an animal model of osteoarthritis. Toxicol Pathol. 2003;31(6):619-24. https://doi.org/10.1080/714044694
- Marker CL, Pomonis JD. The monosodium iodoacetate model of osteoarthritis pain in the rat. Methods Mol Biol. 2012;851:239-48.
- Wang ZM, Chen YC, Wang DP. Resveratrol, a natural antioxidant, protects monosodium iodoacetate-induced osteoarthritic pain in rats.Biomed Pharmacother. 2016;83:763-70. https://doi.org/10.1016/j.biopha.2016.06.050
- Junker S, Krumbholz G, Frommer KW, Rehart S, Steinmeyer J, Rickert M, Schett G, Muller-Ladner U, Neumann E. Differentiation of osteophyte types in osteoarthritis - proposal of a histological classification. Joint Bone Spine. 2016;83(1):63-7. https://doi.org/10.1016/j.jbspin.2015.04.008
- Takahashi I, Matsuzaki T, Hoso M. Long-term histopathological developments in knee-joint components in a rat model of osteoarthritis induced by monosodium iodoacetate. J Phys Ther Sci. 2017;29(4):590-7. https://doi.org/10.1589/jpts.29.590
- Jiang L, Li L, Geng C, Gong D, Jiang L, Ishikawa N, Kajima K, Zhong L. Monosodium iodoacetate induces apoptosis via the mitochondrial pathway involving ROS production and caspase activation in rat chondrocytes in vitro. J Orthop Res. 2013;31(3):364-9. https://doi.org/10.1002/jor.22250
- Gross A. BCL-2 family proteins as regulators of mitochondria metabolism. Biochim Biophys Acta. 2016;1857(8):1243-6. https://doi.org/10.1016/j.bbabio.2016.01.017
- Zhnag HD. Protective effect of diacerein on MIA-induced injury in rat osteoarthritis chondrocytes. Chongqing Medicine. 2016;45(8): 1019-1021.